Pacific Image Electronics Glite 965BW Manual de usuario

User’s Manual

Glite 965BW Gel Doc System

Table of Contents:

PACKAGE CONTENTS………………………………………………………………………………………………………3

SCANNER COMPONENTS………………………………………………………………………………………………..3

SETUP…………………………………………………………………………………………………………………………….4

TO SCAN A FLUORESCENCE-STAINED GEL………………………………………………………………………..5

TO SCAN WESTERN BLOT/COLORIMETRIC STAINED GEL………………………………………………….6

BROWSE SCANNED IMAGES/DELETE SCANNED IMAGES

Examine Scanned Images…………………………………………………………………………………….7

Delete Scanned Images……………………………………………………………………………………….8

INSTALLATION OF AMBER FILTER SHEET………………………………………………………………………….9

DISPLAY SCANS ON MONITOR OR PROJECTOR……………………………………………………………….10

FUNCTION MENU OPTIONS

A. Function Menu Options I………………………………………………………………………………11

B. Function Menu Options II……………………………………………………………………………..13

C. Function Menu Options I: Operation…………………………………………………………….14

D. Function Menu Options II: Operation…………………………………………………………...18

SPECIFICATIONS………………………………………………………………………………………………..…………..22

PACKAGE CONTENTS

» Scanner unit

» User’s manual

» QIG

» Gel shovel

» A/C adapter (12V, 4.16A, DC)

» Amber filter sheet (in box)

» Black plastic sheet (in box)

» Flash Drive (16G) USB. 3.0

SCANNER COMPONENTS

Front (Figure 1):

» Up/Down, Left/Right Menu Button

» USB 2.0 Port

» Scan Button

» Blue Light/White Light Selection Switch

» Power On/Off Button

» LCD Display

» Upper Lid

Back (Figure 2):

» DC power inlet

» USB 2.0 port

» VGA Out port

Up/Down, Left/Right menu button

USB 2.0 Port

Scan button

Blue Light/White Light selection switch

Power Button

LCD display

Upper lid

Figure 1

Figure 2

USB 2.0 port

DC power

Inside (Figure 3):

» Upper Lid

» Lighting Area

» Lid Sensor Figure 3

Upper lid

Lighting area

VGA Out Port

Lid Sensor

SETUP

1. Remove all tapes that hold items in place for shipping.

2. Place the Glite 965BW unit on a flat surface.

3. Connect the included AC adapter to the back of the unit then plug into an electrical outlet.

4. Push power button.

5. Insert a USB flash drive.

6. The Menu Mode icon will be displayed on top left side of the display (Figure 4).

7. Place a gel to the center of the surface of the lighting area (Figure 5, 6).

8. Adjust image conditions then scan.

Figure 5

Place the gel directly

Menu mode icon

Figure 4

Place gel to the center of the lighting area

Figure 6

TO SCAN A FLUORESCENCE-STAINED GEL

1. In “Scan Mode” lift the lid to open the

scanner.

2. Slide the black plastic sheet into the groove of the

white backlight to block the white light on the lid*

(Figure 7).

3. Close the scanner lid.

4. Slide the Light Selection Switch on the front side

of the scanner to choose blue light source for

scanning stained gel.

5. Set and adjust the desired image quality through

the Function Menu if necessary (Please check

“Function Menu Options” section for the details of

all functions).

6. Press SCAN button, green LED will ﬂash and

“CAPTURING” will be displayed on the screen (If

thermal printer has been connected to the

scanner, “Printing Yes/No” option will be

displayed on the screen.). The scanned image will

be automatically saved to the USB flash drive.

7. Scanning of the gel sample is now complete.

(*It is suggested to slide the black plastic sheet to block the white backlight on

the lid for fluorescence stained gel scanning.)

Figure 7

Slide the black plastic sheet to block the background light for

fluorescence stained gel scanning.

Black plastic sheet

White backlight

TO SCAN WESTERN BLOT/COLORIMETRIC STAINED GEL:

1. Slide the Light Selection Switch on the front side of the scanner to select the white light

source for stained gel scanning.

2. In “Scan Mode” , lift the lid to open the scanner.

3. Make sure there is no black plastic sheet blocked in front of the white back light.

4. Place the imaging subject to the center of the lighting area.

5. Close the scanner lid.

6. Set “White Epi”or “White Back Light”* item through Function Menu (Please check “LED

Lighting” in “Function Menu Options” section for turning on “White Epi” or “White Back

Light” options).

7. Adjust the desired image quality through the Functions Menu if necessary (Please check

“Function Menu Options” section for the details of all functions).

8. Press SCAN button, green LED will flash and “CAPTURING” will be displayed on the screen

(If thermal printer has been connected to the scanner, “Printing Yes/No” will be displayed

on the screen.).

9. The scanned image will automatically be saved to USB flash drive.

Scanning of the blot is now complete.

* “White Back Light” mode is to scan the gel such as SDS-PAGE stained with coomassie blue and further in a

transparent background preservation. 7

BROWSE SCANNED IMAGES/DELETE SCANNED IMAGES

Examine Scanned Images

a. When icon displays on top left corner of the screen, press “OK” to get into

functions menu.

b. When “Scan Mode” icon is highlighted , press “OK” again to enter “Play Mode”.

c. The “Play Mode” icon will display on top left corner of screen. The captured

images can then be reviewed one by one by pressing right/left arrow buttons.

d. Press up/down arrow button, the image can be zoomed in/out and be moved

upward/downward and leftward/rightward.

BROWSE SCANNED IMAGES/DELETE SCANNED IMAGES

Delete Scanned Images

a. When viewing the image that needs to be deleted, press “OK” to get into

Function Description II.

b. Press right arrow button to choose “Edit Mode”

c. Press up/down arrow button to choose “Delete”. Press right arrow button to select (yes/no)

to delete the file.

d. Press up/down arrow button to select “Yes”. Press “OK” to save the setting. The image

is thus deleted.

INSTALLATION OF AMBER FILTER SHEET

Glite 965BW is equipped with an amber filter sheet

for blue light protection and gel cutting purposes.

Open the lid and install the filter sheet so the gel

cutting task can be conducted.

1. Open the lid, blue light will automatically turn off.

2. Lift the lid to its maximum opening angle

3. Position the hole of the amber filter sheet in front

of the hanging tab before hanging on the lid

(Figure 8).

4. Place the amber filter sheet onto the filter

hanging tab so the filter sheet hangs as shown in

Figure 9.

5. After hanging the filter on lid, make sure the two

bent areas placed against the lid.

6. Once the installation is completed, one may turn

on the blue LED by pressing “Scan button” for

further extensive experimental procedures.

Figure 8

Figure 9

Filter sheet hanging tab

Amber Filter sheet

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