Olympus FV3000 Manual de usuario

FV3000

Operation Manual

Contents

-----------------Image Acquisition---------------

Starting the System ――――――――――――――――― 4

Visual observation with microscope ―――――――――― 5

XY Image Acquisition ――――――――――――――――― 6

XYZ Image Acquisition ―――――――――――――――― 8

XYT Image Acquisition ―――――――――――――――― 9

4D（XYZT）Image Acquisition ―――――――――――― 10

XYT Image Acquisition（with Z drift compensation） ―― 11

Multi Area Time Lapse ―――――――――――――――― 12

Multi Area Time Lapse using Map Image（１）――――― 14

Tips of Image Acquisition ――――――――――――――― 17

Exiting the system ―――――――――――――――――― 18

Conﬁguration ―――――――――――――――――――― 19

------------------Processing Image ----------------

2D view and operation ――――――――――――――― 21

3D view and operation ――――――――――――――― 22

Creating the movies ――――――――――――――――― 23

Processing: Projection ―――――――――――――――― 24

Exporting the image ―――――――――――――――― 25

Reloading and saving Observation Methods ―――――― 26

------------------Spectral Imaging------------------

Lambda Series（acquiring by single channels）―――― 28

Lambda Series（acquiring by multiple channels）――― 30

Processing: Unmixing（１） ――――――――――――― 32

Processing: Unmixing（２） ――――――――――――― 33

Processing: Unmixing（３） ――――――――――――― 34

Image Acquisition

Starting the system

Turning ON the power

① Set the main switch of the PC to ON

② Switch on the central power..

Laser controller

③ Switch on the main power of each

laser. And Rotate the start key.

Touch Panel Controller（TPC)

④ Press the main switch of the touch

panel controller to set it to ON.

Mercury burner for the light source

⑤ Press the lamp switch on the front side

of the light source.

Logon Windows

⑥ Logon Windows with your own user ID

and password.

Starting software for FV3000

⑦ Double-click the [FV31S-SW] icon on

the desktop.

※Start the software after “Start operation”

is displayed in TPC.

⑦ The stage setting dialog box appears.

Set each checkbox and click [OK].

Factory Default ID & password

Factory User ID：olympus

Password：olympus

Switch on the

main power

Rotate

start key

③Laser Combiner

TPC main

switch

④Touch Panel Controller（TPC)

ON/OFF

switch

To be blue

⑤Mercury burner

②Central

power

①PC

switch

Selection whether or not to initialize the

resonant scanner

XY stage type

Selection whether or not to control the XY

stage by the software

Selection whether or not to perform the

centering in XY direction

Visual observation with microscope

★Fluorescence observation

*Tap the button of the TPC to select

the objective for observation.

① Tap the light path selection button

of the touch panel controller.

(or select “Ocular” in [Ocular] Tool

Window. )

② Tap the button.

③ Tap the [EPI(or EPI1) tab.

④ Select the mirror unit suitable for the

fluorescent probe for observation.

⑤ Open the shutter of the reflected

illumination light on the touch panel

controller.

⑥ Select the ND filters to adjust the

brightness.

※Brightness can be adjusted by rotating

the light volume adjustment dial of

the light source.

Rotate the light volume

adjustment dial

⑦ Adjust the focus

using the focus

knob.

★Transmitted DIC observation

*Tap the button on the TPC to

select the objective for observation.

① Tap the light path selection button

of the touch panel controller.

② Tap the button.

③ Tap the [DIA] tab.

④ Tap the [Elements] tab

and check whether

the [Polarizer] is “IN”.

⑤ Insert the DIC prism

slider.

⑥ Open the shutter of the transmission

illumination light on the touch panel

controller.

⑦ If necessary, tap the [Brightness] tab

and adjust the brightness.

⑧ Adjust the focus and contrast.

④

①

②

⑤

④

③

⑥

①

②

⑦

③

⑥

Adjust the DIC contrast

by rotating the knob.

XY Image Acquisition(1)

① Select “VBF” in the [PMT Setting] Tool Window.

Assigning the detector to channel

② Press the button on [PMT Setting]

Tool Window to open the [Dye & Detector Select]

dialog box.

③ Press the button to reset the Assigned

Dye.

④ Double-click the name of the fluorescence dye to

observe.

⑤ When registering a fluorescence dye, TD channel

is registered automatically. If you don’t need,

double-click the TD channel to cancel it.

⑥ If you want to observe with multiple phases using

the virtual channel scan, press the button

to add the phase. And drag&drop the selected

“Dye” to the observation channel list of the phase

you want to add.

⑦ After setting all channels, press the [OK] button.

Adjusting the live image

⑧ If you want to acquire multiple channel image,

select Sequential scan “Line” in the [PMT Setting]

Tool Window.

⑨ Press the button on the [Live] window.

Adjust focus and set Laser Intensity(%),

Sensitivity(HV), Gain and Offset on [PMT setting]

Tool Window.

⑩ If you use virtual channel scan, press

to switch phases. On both phases, adjust focus

and set Laser Intensity(%), Sensitivity(HV), Gain

and Offset.

Virtual Channel Scan

This mode allows to acquire multiple CH image

whose channel number is larger than the

number of detector.

①

Adjusting Confocal

Aperture.

Reducing noises by

Averaging.

②

③

④

⑤

⑦

⑧

⑨

Laser Intensity

Sensitivity

⑩

【Hi-Lo Mode】

pixels of intensity 4095 are shown in red.

Pixels of intensity 0 are shown in red.

Slide 6

u5 確認（英語のほう）

元島ゆき; 03.02.2017

XY Image Acquisition(２)

Setting the scanner

⑩ On [LSM Imaging] Tool Window, select the

scan method in [Type] and [Mode].

※See 23 pages for details.

⑪ Set scanning speed.

⑫ Specify “Image Size” to set the scan area.

⑬ Set the zoom, rotation and clip scan if

needed.

Starting Acquisition

⑭Select [Normal] tab in [Acquire] Tool Window.

Press the button to open the dialog

box, and select the folder to save the images.

*The acquired images are saved automatically.

Serial number is added at the end of file name

like “***_0001” and “***_0002”.

⑮Press button to start acquiring the

image.

DIC observation

1. Insert the DIC slider to microscope.

2. Register the TD channel.

3. Set the laser to be used for observation.

4. Adjust the sensitivity(HV).

5. Adjust the contrast by rotating the knob of

the DIC slider.

(See page 3 ⑤ in left side. )

Attention

Check [LSM] in [Series] Tool Window whether it

is selected “OFF” [Z] and [Time] .

⑩

⑪

⑫

⑬

⑮

⑭

XYZ Image Acquisition

*Before starting the following procedure, make

adjustments for XY imaging.

(refer to page 4-5.)

Setting Z series

① Select “ON” in [Z] on [Series] Tool Window.

② Select “Start/End” in [Motor] on [Z section].

③ Change the Z position by rotating the

focusing knob or pressing the

button. Press the button in [Start]

at the Z position to start acquiring the

image.

④ Change the Z position and go on to press

the button in [End] at the Z position

to end acquiring the image.

⑤ Enter a value in [Slices] or [Step Size].

Setting one will set other automatically.

Pressing the button, both numerical

values “Slices” and “Step Size” is optimized.

Starting Acquisition

⑥ Select [Normal] tab in [Acquire] Tool

Window. Press the button to display the

dialog box, and select the folder to save the

images.

Press the button to start acquiring

the image.

Finishing Acquisition

⑧ Finishing acquisition, buttons blink.

Press the button to complete the

image acquisition. If you want to Additional

images under the same condition, enter the

number of additional acquisition and press

the button. After the image is

acquired, press the button.

①

③

⑤

②

④

⑥

⑦

⑧

:Each click shifts the position by the value

entered for Step Size

:Each click shifts the position by ½ the value

entered for Step Size.

Pressing the button, current

position is registered as origin.

Slide 8

u6 新しく追加。要チェック。

元島ゆき; 08.02.2017

Step Size

にしたけど大丈夫だろうか？

内容・英語ともに。

元島ゆき; 08.02.2017

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