Olimpus FLUOVIEW FV500 Manual de usuario

User’s Manual
FLUOVIEW
FV500
CONFOCAL LASER SCANNING
BIOLOGICAL MICROSCOPE
Ver.3.3
This user’s manual is for the software to be run on Olympus FLUOVIEW FV500 Confocal Laser
Scanning Biological Microscope. To ensure safety, obtain optimum performance and familiarize yourself
fully with this product, we recommend that you study this manual thoroughly before operation.
This user’s manual is composed of two volumes including “SYSTEM OVERVIEW”, “OPERATION
INSTRUCTIONS”, “MAINTENANCE” and “TROUBLESHOOTING”. Together with this manual, please
also read the “User’s manual FLUOVIEW FV500” and the instruction manual of the microscope in order
to understand overall operation methods. To ensure the safety operation of laser system, we
recommend you to study the manual of each laser and the light source equipment besides this manual.
Retain this manual in an easily accessible place near a system for future reference. AX9787
Petition


1
Page
CAUTION
1. Reproduction, copying or duplication of a part or all of this software and manual is prohibited.
2. The information described in this manual may be subject to change without notice.
Registered Trademarks
Microsoft, Microsoft Windows, Excel for Windows are registered trademarks of Microsoft Corporation.
Other brand names and product names are trademarks or registered trademarks of their respective owners.

FLUOVIEW MANUAL CONFIGURATION
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FLUOVIEW MANUAL CONFIGURATION
The FLUOVIEW system uses two manuals including this “User’s Manual” and the on-
screen manual built into the software (“Online Help”).
The User’s Manual is composed of the five following volumes and subject matter:
•SAFETY GUIDE (Anoter manual)
Describes notes and cautions on using the FLUOVIEW system and on types of
warning labels.
•SPECIFICATIONS (Another manual)
Describes the specifications of the FLUOVIEW system.
•SYSTEM OVERVIEW (This manual)
Describes the outline of the FLUOVIEW system.
•OPERATION INSTRUCTIONS (This manual)
Describes the operation procedures of the FLUOVIEW system, for example,
methods for image acquisition and various image processing.
•MAINTENANCE (This manual)
Describes maintenance of the FLUOVIEW system.
•TROUBLESHOOTING (This manual)
Describes countermeasures in case trouble occurs.
For Online Help, please see “1-3 Online Help” in “OPERATION INSTRUCTIONS” of this
manual.

NOTATIONS IN THIS MANUAL
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Page
NOTATIONS IN THIS MANUAL
This manual complies with the following notations.
Notation of Caution, Notes and Tips
Notation Description
Caution to prevent injuries to the user or damage to the product
(including surrounding objects).
NOTE
Note for the user.
TIP
Hint or one-point advice for user reference.
Notation of panel, Command Buttons and Dialog Boxes
Notation Description
[Acquire] panel The name of a panel, dialog box, list box or check box is
enclosed inside square brackets ([ ]).
<OK> button
<Open File> button
The name of a button in a panel or dialog box is enclosed
inside angled brackets (< >).
Notation of Mouse Operations
Notation Description
clicking Action of pressing, then immediately releasing the mouse
button.
double-clicking Action of clicking the mouse button twice in quick succession.
dragging Action of moving the mouse while holding down the mouse
button, then releasing the mouse button at the desired
destination.
(Note) In this manual, clicking, double-clicking and dragging involves pressing the left
button of the mouse, unless otherwise specified.

NOTATIONS IN THIS MANUAL
4Page
Notation of key operations
Notation Description
Enter The name of a key is enclosed inside .
F1
+
Alt The positive sign (+) expresses the combination of more than
one key operation.
For example, F1
+
Alt refers to pressing the
F1 key while holding the Alt key down.
Direction keys Generic names given to the →, ←, ↑and
↓keys.
Notation of system-specific terms
Notation Description
XY observation
(Other observations)
Refers to observation with XY scanning.
(The same principle also applies to other observations such
as XZ, Xt, XYZ, XYt and XYZt.)
Note that some of the panels and dialog boxes shown in this manual are not the precise
reproductions of the originals. Some windows are resized to facilitate the reading and
some grayed-out characters are printed in readable characters.

This volume describes the outline of the FLUOVIEW FV500
system.
Please read this volume so that you can understand the
system before use.
On This Volume
S
SY
YS
ST
TE
EM
M
O
OV
VE
ER
RV
VI
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EW
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CONTENTS
1 SYSTEM OVERVIEW 1-1
1-1 Principle ............................................................................................1-1
1-2 Features of FLUOVIEW FV500 ........................................................1-2
1-3 Light Path Diagram ..........................................................................1-3
1-4 System Configuration ......................................................................1-4
1-4-1 System Component Units and Their Functions ........................................... 1-4
1-4-2 Block Diagram............................................................................................. 1-6
1-5 Software Functional Configuration ................................................1-9
1-5-1 Function Panel and Display Panel............................................................... 1-9
1-5-2 Panel Structure of the Software................................................................. 1-10
1-5-3 Icons Executed by Dragging & Dropping................................................... 1-11
1-5-4 Identification of Images Depending on the Observation Methods.............. 1-12

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