Nikon Elcipse TE2000 Manual de usuario

Turning on the system:

1) Turn on the main switch (A).

2) Turn on the computer (B).

3) If you need the mercury lamp,

turn on the switch (C) and then

press ignition (D).

4) Log onto the computer through

“MIC users” and start the

“NIS-Elements” software.

Turning off the system:

1) If you are the last user of the day, or if there is

more than 30 min interval before the next user is

booked, turn off the mercury lamp (C).

2) Close the software and “turn off” the computer

through the start menu.

3) Turn off the main switch (A).

4) Fill out the logbook.

Nikon TE2000 fluorescent microscope

Filter wheel with different

positions. Nr 6 is empty

and used for imaging DIC.

Shutter

for the

UV light

Light path control. If you are

looking at your sample (A),

taking fluorescence/DIC

images (B) or taking color

images (C).

B C

Control over the halogen light.

(The main switch on the halogen power

supply need to be on.)

Regulate the

intensity here.

Switching between

objectives. Choose the right

objective for your sample.

Which camera do I choose?

Nikon DS-U1 (color) camera - or - MuTech MV 1500 (B/W) camera

Turn on main switch before use.

Adjusting the Köhler illumination

-this will increase image contrast-

1. Decrease the size of the diaphragm (A) so that you can see the

edges.

2. Bring the edges of the field iris onto focus by raising or lowering

the condensor focusing knob (B).

3. Senter the image of the field iris by using the condensor sentering

knobs (C).

4. Open the diaphragm so the edges lie just beyond the field of

view.

The difference between the objectives

HMC - Hoffman modulation contrast (objectives in position 5-6). The method converts phase

gradients into intensity variations to create 3D-like images, allowing observation in plastic dishes.

These objectives are suited for plastic dishes.

MC 30,55HMC LWD 40X

MC 20,40HMC LWD 20X

Ph23,7-2,70,60CFI Plan Fluor ELWD 40X

Ph10,40ELWD 20X

Ph 116,00,30CFI Plan Fluor 10X

17,10,13CFI Plan Fluor 4X

RemarksWD (mm)NADescription

Possit

ion

Correction collar. You have to adjust

the ring to the coverslip-petridish

thickness. Turn until the image looks

the sharpest.

Indicates the phase-ring. Use ”Ph2” for

this objective.

Indicates the NA. The higher the

number the better the resolution.

Correction collar. You have to adjust

the ring to the coverslip-petridish

thickness. Turn until the image looks

the sharpest.

Indicates the phase-ring. Use ”Ph2” for

this objective.

Indicates the NA. The higher the

number the better the resolution.

Collar not corrected Collar corrected

Use your fingers to turn the collar slightly. Then adjust

the focus. Now keep turning the collar until you get the

best sharp focus. This is very important for the quality

of your image.

Adjusting the correction collar on the objective

1) Start the NIS-Elements software.

2) If the stage/joystick is not responding,

make sure the stage is in “auto” position

and connect the stage through the

software.

“Devices” – “manage devices”, scroll

down to the “Märzhauser” and then click

“connect”.

1) If you want to acquire images

of multiple fluorochromes or

combine fluorescence and DIC

images – chose “capture

multichannel image” –

“multichannel setup”.

2) Tag off the settings you need and

click ok.

3) You are now ready to start

imaging. Click the multichannel

icon (left side) and remember to

go from “visual” to “camera

mode”.

Setting up a multichannel acquisition

1) Initiate stage and make sure

nothing is interfering (objectives

must be down) with the stage as it

will move to the outer limits.

2) Go to “acquire-large scan” and

define the image size you need.

Make sure you are located at the

upper left of your sample before

you start scanning.

3) Position the first frame in the top

left corner. 3 x 2 will give you a

large image as seen under.

Acquiring a large image with automated stage

1 2 3

6 5 4

Troubleshooting

•The stage/joystick is not responding.

The stage has not been initialized because it was on manual when

the software was opened. Make sure the stage is in “auto”

position and connect the stage through the software.

“Devices” – “manage devices”, scroll down to the “Märzhauser” and

then click “connect”.

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