NEW BRUNSWICK SCIENTIFIC AF 2000 Manual de usuario

AF 2000

Anti–Foam Control Module

MANUAL NO: M1230–0053

Rev E

March 4, 1998

NEW BRUNSWICK SCIENTIFIC CO., INC.

BOX 4005 . 44 TALMADGE ROAD . EDISON, NJ 08818-4005

TELEPHONE: . 732-287-1200 . 800-631-5417 . FAX: 732-287-4222

TELEX: 4753012 NBSCO . INTERNET: http://www.nbsc.com

E-MAIL: [email protected]

UNITED KINGDOM THE NETHERLANDS BELGIUM GERMANY

New Brunswick Scientific (UK) Limited New Brunswick Scientific Benelux B.V. New Brunswick Scientific NV / SA New Brunswick Scientific GMBH

Edison House–163 Dixons Hill Road P.O Box 6826 ‘t Veldeke, 1 B-1970 In Der Au 14

North Mymms Hatfield Hertfordshire 6503 GH Nijmegen Wezembeek-OPPEM D-72622 Nürtingen

AL9 7JE United Kingdom Holland België/Belgique Deutschland

Tel: 07072 75733 Tel: 080 3779179 Tel: 02 731 67 87 Tel: 07022 932490

Fax: 07072 67859 Fax: 080 3787755 Fax: 02 731 81 30 Fax: 07022 32486

Telex: 94018208 NBSC G

CAUTION !CAUTION !

Operate the AF 2000 as described in this manual.Operate the AF 2000 as described in this manual.

If Operational Guidelines are not followed, equipment damage can

occur. Please read the entire User’s Guide before attempting to use

the AF 2000.

If Operational Guidelines are not followed, equipment damage can

occur. Please read the entire User’s Guide before attempting to use

the AF 2000.

AF 2000 User’s GuideAF 2000 User’s Guide

New Brunswick Scientific Company, Inc.

Box 4005 44 Talmadge Road

Edison, New Jersey 08818–4005

Reproduction, adaptation or translation without prior written permission from

New Brunswick Scientific is prohibited.

Disclaimer Notice

New Brunswick Scientific Company Inc., reserves the right to change information

in this document without notice. Updates to this information reflect our

commitment to continuing product development and improvement.

Manual Conventions

#NOTE: Notes contain important and useful information separate from text.

Caution messages appear before procedures which, if not

observed, CAUTION ! could result in damage to the equipment.

Warning messages alert you to specific procedures or practices

WARNING ! which, if not followed correctly, could result in serious personal

injury.

Bold Text in Bold face type emphasizes key words or phrases.

New Brunswick Scientific Co., Inc.

Every Instrument manufactured by the New Brunswick Scientific

Co., Inc. is warranted to be free from defects in material and work-

manship. This apparatus with the exception of glassware, lamps and

electrodes (where supplied), is warranted for 1 year against faulty com-

ponents and assembly and our obligation under this warranty is limited

to repairing or replacing the instrument or part thereof, which shall

within 1 year after date of shipment, prove to be defective after our

examination. This warranty does not extend to any NBS products

which have been subjected to misuse, neglect, accident or improper

installation or application; nor shall it extend to products which

have been repaired or altered outside the NBS factory without

prior authorization from New Brunswick Scientific Co., Inc.

W A R R A N T Y

AF 2000 User’s Guide

CONTENTS

1. OVERVIEW................................................................................................................. 8

1.1 SPECIFICATIONS......................................................................................................9

2. INSPECTION, VERIFICATION & UNPACKING OF EQUIPMENT............................. 9

2.1 INSPECTION OF BOXES............................................................................................. 9

2.2 PACKING LIST VERIFICATION..................................................................................... 9

3. PREPARING THE LOCATION................................................................................. 10

3.1 PHYSICAL LOCATION..............................................................................................10

3.2 ENVIRONMENT....................................................................................................... 10

4. UNPACKING & INSPECTION.................................................................................. 11

4.1 UNPACKING THE EQUIPMENT..................................................................................11

4.2 INSPECTION OF EQUIPMENT .................................................................................... 11

5. ANTI–FOAM MODULE FEATURES......................................................................... 13

6. GETTING STARTED ................................................................................................ 16

6.1 STACKING CONTROL MODULES............................................................................... 17

6.2 ANTI–FOAM MODULE INTERCONNECTING CABLE CONNECTION.................................. 18

7. PREPARATION BEFORE THE AUTOCLAVE......................................................... 19

7.1 VESSEL HEADPLATE ASSEMBLY.............................................................................. 19

7.2 PREPARING THE CONDUCTANCE PROBE FOR THE AUTOCLAVE................................... 23

New Brunswick Scientific Co., Inc.

8. FERMENTATION SETUP......................................................................................... 24

8.1 CONDUCTANCE PROBE LEAD CONNECTION.............................................................. 24

8.2 SENSITIVITY ADJUSTMENT...................................................................................... 24

8.3 ANTI–FOAM CONTROL ...........................................................................................25

8.4 HIGH FOAM CONTROL............................................................................................ 25

8.5 DISPLAYING THE CURRENT VALUES......................................................................... 27

9. PREVENTIVE MAINTENANCE................................................................................ 27

9.1 CLEANING PROCEDURES........................................................................................ 28

9.2 FUSE REPLACEMENT.............................................................................................. 28

10. TROUBLESHOOTING............................................................................................ 30

11. REPLACEMENT PARTS AND ACCESSORY INFORMATION ............................. 31

11.1 REPLACEMENT PARTS DESCRIPTIONS AND PART NUMBERS..................................... 31

11.2 ACCESSORY DESCRIPTIONS AND CONDUCTANCE PROBES PART NUMBERS ..............31

APPENDIX A Control Schematic–AF 2000.................................................................. 32

INDEX...........................................................................................................................33

AF 2000 User’s Guide

This page intentionally blank.

New Brunswick Scientific Co., Inc.

1. OVERVIEW

The New Brunswick Scientific's AF 2000 Anti–Foam Module is an optional unit that

connects to the BioFlo 2000 Fermentor. The Anti–Foam Module regulates the operation

of a self contained feed pump that delivers anti–foam agent and limits the agitation rate

when high foam condition occur.

To operate the Anti–Foam Module you must purchase a Conductance Probe and

Conductance Probe Leads separately. The length of the Conductance Probe

should be selected to match the size of your fermentation vessel.

AF 2000 User’s Guide

1.1 SPECIFICATIONS

AF 2000

Dimensions 9½″x 10½″x 3 5/16″

(241 mm x 267 mm x 84 mm)

Electrical 24 Volts AC–for Control Module

100–240 Volts–for BioFlo 2000 Fermentor

Pump 0–6.35 mL/min.

SetPoint Range 0–100%

Pump Head Watson Marlow 101

Weight 7 lbs. (3 kg)

2. INSPECTION, VERIFICATION & UNPACKING OF EQUIPMENT

2.1 INSPECTION OF BOXES

After you have received your order from New Brunswick Scientific, inspect the

boxes carefully for any damage that may have occurred during shipping. Report

any damage to the carrier and to your local NBS Sales Order Department.

(732) 287-1200 / 800-631-5417.

2.2 PACKING LIST VERIFICATION

Verify on your NBS packing list that you have received the correct materials.

New Brunswick Scientific Co., Inc.

3. PREPARING THE LOCATION

3.1 PHYSICAL LOCATION

The surface where you place the Anti–Foam Module should be smooth, level and

sturdy. All Control Modules should be stacked to the immediate right or left of

the BioFlo 2000 Fermentor. Ensure that there is enough space around the back

and the front of the Anti–Foam Module for proper operation.

CAUTION !

When stacking additional Control Modules, the pH 2000 should

always be on the bottom. When the pH 2000 is on the bottom and

an acid or base spill occurs, the spill will not damage

additional Modules.

3.2 ENVIRONMENT

The Anti–Foam Module operates properly under the following conditions:

•ambient temperature range 10°C–35°C

•relative humidity up to 80% non-condensing

•voltage fluctuation ±10%

AF 2000 User’s Guide

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