NanoEnTek JuLi Stage Manual de usuario

JuLI™Stage, Real-Time Cell History Recorder 1

User Manual

All the materials in this user guide are protected by Korean and international

without the permission of the copyright owner.

JuLI™ Stage User Manual

Website : www.julistage.com

E-mail : [email protected]

Manufactured by : NanoEnTek Inc.

851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea

Tel : +82-2-6220-7940

Fax : +82-2-6220-7999

The information in this manual is described as accurately as possible. The changes

involved in rmware and software may be applied without notication.

Documentation: NESMU-JST-001E

Revision history: V.0.0 Date: OCT 2014

V.0.5 Date: MAY 2015

V.1.0 Date: SEP 2015

V.1.5 Date: MAR 2016

V.2.0 Date: MAY 2017

JuLI™Stage, Real-Time Cell History Recorder 3

Table of contents

Product components

Product overview

Features

Description

- Front view

- Side view

- Rear view

- Control box

Installation

- Unpacking the instrument

- Remove the lter change

lock module

- Install the instrument

- Place the instrument

- Operating environment

- Cautions

User interface

- GUI

Basic operation

- Moving

- Selection

- Position zoom

- Zoom

- Focus

- Size bar

- Auto Focus

Operation

- Preview menu

- Option menu

- Data menu

- Setting menu

Quick operation guide

- Position Calibration (for Vessel type)

- Stitching Image Calibration (Stitching & Map mode)

- Image capture, save and overlay

- Operation type-1

(for Selecting multiple samples and use the Auto focus)

- Operation type-2

(for Select single samples and use the Manual focus)

Cleaning and Maintenance

Troubleshooting

Warranty

Safety precautions

Product specications

Ordering information

Technical support

The JuLI™ Stage, Real-Time Cell History Recorder is shipped with the following components.

Please check all components listed below when the instrument is delivered.

PC will be shipped separately because it is optional.

If any item is missed of damaged, contact your local distributor or e-mail [email protected].

Product components

JuLITM Stage main instrument

1 EA

1 SET

1 EA 1 EA

5M, 2EA

2 EA 3 EA

10x lens is factory-installed with the

JuLI™ Stage main instrument.

2 EA 4 EA

Control Box

User manual Quick manual

Repeater

Connection Cable Objective lens (4x, 10x, 20x)

Power cord with 4 adaptor cords

(4 pcs/set, for U.S./Canada/Taiwan/Japan,

Europe or UK)

USB Cables (20 ~ 43 cm)

NanoEnTekInc. (HQ) 5,Digital-ro 26-gil, Guro-gu, Seoul, 152-740, Korea / Tel: +82-2-6220-7940, Fax:+82-2-6220-7721

NanoEnTekUSA Inc. 5627 Stoneridge Drive Suite 304, Pleasanton, CA 94588, USA / Tel:+1-925-0108, Fax: +1-925-225-0109

www.nanoentek.com/ [email protected]

NESMQ-JST-001E(V.0.0)

Quick manual

User Manual

2 EA

Ferrite core

JuLI™Stage, Real-Time Cell History Recorder 5

Product overview

A fully-automated, real-time cell history recorder that acquires cell images directly from various cell culture plates (6 to

384 well) slides and dishes in an incubator. JuLI™ Stage supports multi-channel uorescent colors, multiple objective

lenses, and sensitive lter-based optics to optimize for various live cell assays. In addition, it enables users to support

quantied cellconuence results with low variation and the growth curve using image-based analysis with bright eld.

Compact, compatible and Real-Time Cell History Recorder of live cell imaging from inside your cell

culture incubator!

Suitable for the following applications:

• Live cell imaging (time lapse) • Cell growth monitoring

• Cell culture quality control • Cell culture optimization

• HCS cell imaging analysis (6 to 384 well) • Stem cell development

• Cell-based assay optimization • Cell proliferation

• Image stitching • Cell cytotoxicity

• Angiogenesis • Cell migration study

• Scratch-wound assay • Cell dierentiation

• Neurite growth

• Angiogenesis

• Protein expression

• Kinetic imaging and cell dierentiation

Features

The JuLITM Stage a Real-Time Cell History Recorder, oers you the following benets,

Automated imaging system

The JuLI™ Stage provides a live cell imaging using automatic focus and a precise one by one multi-well control system.

- Fully automated and motorized X-Y scanning stage

- Auto focusing on every single position

- Image tiling with a stitching function

Sensitivity and expandability

The sensitive CCD camera, multi-channel uorescence and magnication (4, 10 & 20 X) provide the powerful detection

capabilities required for various cell-based assays.

- High-sensitivity monochrome CCD

- Multi-channel uorescence imaging (3 color uorescence GFP, RFP and DAPI and bright channel)

- User-changeable objective lens (4, 10 & 20 X)

Versatile and Powerful S/W

The JuLI™ Stage software allows you to generate live cell imaging and analysis easily.

- Image capturing and time-lapse recording with multi-position scanning

- Navigation function to gure out the optimal position

- Image stitching function to scan the whole well at once

Compatibility and easy to use

The compact and ready to use system can be operated in an incubator and it can be controlled by the JuLI™ Stage

software from outside.

- Compact and compatible to CO2incubator

- 23” full HD All-in-one touch screen PC control

- Simple steps for the system setup and operations

- Compatibility with various brands of well plates by auto adjustment function

JuLI™Stage, Real-Time Cell History Recorder 7

Description

Front view

Side view

[Front view]

[Side view]

LED (Bright mode)

Vessel holder

Automatic X-Y stage

Lens holder

Cover

Main body

Used to protect test samples from external light. When you use the JuLl™ Stage

in an incubator, the cover can block out light from the outside.

Front view of the JuLl™Stage. The main body is composed of LED, Filters

(Fluorescence mode) and optic parts.

LED (Bright mode)

Vessel holder

Automatic X-Y

stage

Lens holder

LED is used for bright image viewer.

This is a holder for sampling plates. The capacity is 6 well to 384 well.

The automatic X-Y stage makes it possible for you to move to the position

you want.

This is a holder for changeable lens.

Main Body

(LED, Fluorescence Filters & optic parts)

Cover

Description

Rear view Fan

Connection port

Fan

Connection port

Power inlet

Machinery cooling system.

Connect the JuLI™ Stage main instrument with the control box.

Connect the instrument to an electrical outlet using the supplied power

cord and the appropriate plug, based on the electrical outlet conguration

in your country.

[Rear view]

Control box When the PC and the control box are properly connected, the red light is

turned on.

Connect the JuLI™ Stage main instrument with the control box

Control the camera in the JuLI™ Stage main instrument.

Control the JuLI™ Stage main instrument operating.

Serial and camera

LED

Connection port

Serial port

Camera port

Connection port Camera portSerial port

Serial connection LED

Camera connection LED

JuLI™Stage, Real-Time Cell History Recorder 9

1. Unscrew the lter change lock module screw to remove it.

2. Gently pull lter change lock module screw out, away from the instrument.

3. Put the lter in the pouch to keep safe.

IMPORTANT!

Remove the Filter change lock module by turning it counterclockwise to prevent damage

of the instrument before plugging the power cord into power outlet and turning on the JuLI™ Stage.

1. Open the box and remove the material foam from the box.

2. Carefully lift the instrument out of the box, holding it by handholds in the bottom of the base.

3. Place the instrument on a at, level surface (table or incubator).

Do not lift the JuLI™ Stage by condense arm.

Installation

Unpacking

the instrument

Remove the

lter change

lock module

IMPORTANT!

Filter change lock module

Installation

1. Connect repeater to the PC and two USB cable, then connect to control box.

(PC Monitor Resolution: 1920 ×1080 pixels)

1-1. Plug the power cord into the rear side of the PC.

2. Connect the JuLI™Stage instrument and the Control box using the connection cable.

Connect the connection cable and control box by matching the blue and red sticker.

3. Plug the power cord of PC and instrument into the electrical outlet.

2-1. Plug the power cord into the rear of instrument.

☞ Note: Check the connection on the rear side of the PC.

☞ Note: If you connect all the components, it will look like the Image below.

☞ Note: Make sure of connection port direction of control box before connecting.

☞ Note: Be sure to use only the power cord supplied with your instrument.

Powering the instrument with an unapproved power cord may damage the instrument.

Install

the instrument

Power port USB port

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