Molecular Devices GenePix 4300A Manual de usuario
GenePix®4300A/4400A
High-Resolution Microarray Scanner
User Guide
0112-0173 B
October 2010
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This document is provided to customers who have purchased Molecular Devices, Inc.
(“Molecular Devices”) equipment, software, reagents, and consumables to use in the
operation of such Molecular Devices equipment, software, reagents, and
consumables. This document is copyright protected and any reproduction of this
document, in whole or any part, is strictly prohibited, except as Molecular Devices
may authorize in writing.
Software that may be described in this document is furnished under a license
agreement. It is against the law to copy, modify, or distribute the software on any
medium, except as specifically allowed in the license agreement. Furthermore, the
license agreement may prohibit the software from being disassembled, reverse
engineered, or decompiled for any purpose.
Portions of this document may make reference to other manufacturers and/or their
products, which may contain parts whose names are registered as trademarks and/or
function as trademarks of their respective owners. Any such usage is intended only
to designate those manufacturers' products as supplied by Molecular Devices for
incorporation into its equipment and does not imply any right and/or license to use
or permit others to use such manufacturers' and/or their product names as
trademarks.
Molecular Devices makes no warranties or representations as to the fitness of this
equipment for any particular purpose and assumes no responsibility or contingent
liability, including indirect or consequential damages, for any use to which the
purchaser may put the equipment described herein, or for any adverse circumstances
arising therefrom.
For research use only. Not for use in diagnostic procedures.
Product manufactured by Molecular Devices, Inc.
1311 Orleans Drive, Sunnyvale, California, United States of America 94089.
Molecular Devices, Inc. is ISO 9001 registered.
© 2010 Molecular Devices, Inc.
All rights reserved.
Printed in the USA.
The trademarks mentioned herein are the property of Molecular Devices, Inc. or their
respective owners. These trademarks may not be used in any type of promotion or
advertising without the prior written permission of Molecular Devices, Inc.
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GenePix Microarray Scanner User Guide
0112-0173 B 3
Licensing Notice
Molecular Devices is not licensed under any patents owned by Oxford Gene Technology
Limited (“OGT”), covering oligonucleotide arrays and methods of using them to analyze
polynucleotides. The purchase of Molecular Devices products does not convey any license
under any of OGT’s patent rights, including any right to make or use oligonucleotide
arrays under OGT’s patents.
Customers may use Molecular Devices products to analyze oligonucleotide arrays
according to OGT’s patented methods if those arrays have either been purchased from
OGT’s licensed suppliers, or have been made by the customer under a license from OGT.
Please contact OGT to enquire about a license under OGT’s patents at
USE OF THIS INSTRUMENT WITH MICROARRAYS MAY REQUIRE A LICENSE FROM ONE OR
MORE THIRD PARTIES THAT HAVE PATENTS RELEVANT TO SUCH USE. MOLECULAR
DEVICES DOES NOT SUGGEST OR PROMOTE THE USE OF THIS INSTRUMENT IN A
MANNER THAT INFRINGES ON THE PATENT RIGHTS OF A THIRD PARTY. YOU ARE
ENCOURAGED TO EVALUATE WHETHER A LICENSE IS REQUIRED FOR YOUR SPECIFIC
APPLICATION OF THIS INSTRUMENT. COMPANIES THAT HAVE INTELLECTUAL PROPERTY
RIGHTS IN THE POTENTIAL FIELD OF APPLICATION OF THIS INSTRUMENT INCLUDE
WITHOUT LIMITATION, AFFYMETRIX, INC. (“AFFYMETRIX”), AGILENT, AND OXFORD
GENE TECHNOLOGY. THIS INSTRUMENT HAS NOT BEEN LICENSED OR APPROVED FOR
DIAGNOSTIC APPLICATIONS.
THE USE OF THIS INSTRUMENT IN CONNECTION WITH MICROARRAYS MAY BE WITHIN
THE SCOPE OF PATENTS HELD BY AFFYMETRIX. TO THE EXTENT THAT AFFYMETRIX
PATENT RIGHTS ENCOMPASS THIS INSTRUMENT OR ITS USE, AFFYMETRIX HAS
GRANTED A LIMITED PATENT LICENSE FOR RESEARCH USE ONLY AND NOT FOR USE IN
DIAGNOSTIC PROCEDURES. SUCHLICENSE, IF APPLICABLE, IS LIMITED TO USE OF THIS
INSTRUMENT WITH SPOTTED MICROARRAYS SEPARATELY LICENSED BY AFFYMETRIX.
NO LICENSE IS CONVEYED, BY IMPLICATION, ESTOPPEL OR OTHERWISE, TO USE THIS
INSTRUMENT WITH MICROARRAYS MADE USING IN SITU OR PHOTOLITHOGRAPHIC
SYNTHESIS. NO OTHER LICENSE IS CONVEYED, BY IMPLICATION, ESTOPPEL OR
OTHERWISE, UNDER ANY AFFYMETRIX PATENT OR OTHER INTELLECTUAL PROPERTY
RIGHT.
This instrument is licensed by Affymetrix under the following patents: U.S. Patent Nos.
5,578,832;5,631,734; 5,834,758; 5,936,324; 5,981,956; 6,025,601; 6,141,096;
6,171,793; 6,185,030; 6,201,639;6,207,960; 6,218,803; 6,225,625; 6,252,236;
6,262,838; 6,335,824; 6,403,320; 6,403,957, 6,407,858;6,472,671; 6,490,533;
6,545,264; 6,597,000; 6,643,015; and 6,650,411.
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4 0112-0173 B
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0112-0173 B 5
Contents
Foreword . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Who This Manual Is For . . . . . . . . . . . . . . . . . . . . . . . . . 7
Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . 7
Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Scanner Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Scanner Components . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Protective enclosure . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Optics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Lasers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Optical Path. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Emission Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
PMT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Status Lights . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Scanner Specifications . . . . . . . . . . . . . . . . . . . . . . . . . 14
Site Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Chapter 2 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Unpacking the scanner and positioning it on the bench. . . 17
Installing the Software . . . . . . . . . . . . . . . . . . . . . . . . . 20
Turning on the Scanner . . . . . . . . . . . . . . . . . . . . . . . . 21
Inserting a Slide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Shutting Down the Scanner. . . . . . . . . . . . . . . . . . . . . . 23
Chapter 3 Maintenance & Troubleshooting . . . . . . . . . . 25
Moving the Scanner . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Installing Custom Filters . . . . . . . . . . . . . . . . . . . . . . . . 27
Interlock Failure Symptoms . . . . . . . . . . . . . . . . . . . . . 34
Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
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Contents
6 0112-0173 B
Appendix A Warranty and Service . . . . . . . . . . . . . . . . . 35
Standard Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Out-of-Warranty Repair Service . . . . . . . . . . . . . . . . . . 35
Optional Service Agreement. . . . . . . . . . . . . . . . . . . . . 35
Technical Support. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
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0112-0173 B 7
Foreword
The foreword describes the intended audience for the manual. It
defines the typographical conventions used in the manual and lists the
related documentation.
Who This Manual Is For
This manual is written for the GenePix®4300A/4400A High-Resolution
Microarray Scanner user. It contains the information required to install
the scanner, turn it on, and perform maintenance procedures.
Conventions
Within the scope of this manual, the following typographical
conventions are used:
CAUTION! Indicates an operation that may cause damage to the
instrument, device, or data, if the precautions are not followed.
Related Documentation
The customer documentation for the GenePix 4300A/4400A Microarray
Scanner includes the GenePix®Pro Software Reference Guide, Safety
Practices manual, and the GenePix®Pro online Help.
WARNING! A warning indicates an operation that may cause
personal injury if precautions are not followed.
Tip! Provides useful information that helps apply the techniques and
procedures in the text to your specific needs, and provides shortcuts,
but is not essential to the completion of a procedure.
Note: Provides essential information for the completion of a procedure.
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Foreword
8 0112-0173 B
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0112-0173 B 9
1
Introduction
The GenePix®4300A/4400A Microarray Scanner uses a laser-excitation
based fluorescence scanning and imaging system. The laser-based
scanning system uses a patent-pending mechanical design, and
operates by slowly moving the slide in the Y–direction, while the slide is
rapidly scanned in the X–direction. The design of the scanner allows
scanning up to four wavelengths of a 25 mm by 75 mm slide at:
•5 m resolution for the 4300A in approximately 30 minutes for
all four channels
•2.5 m resolution for the 4400A in approximately 60 minutes for
all four channels
Scanner Features
•Up to four internally installed laser-excitation sources
•Gradient neutral density wheel permitting 5 to 100 % laser
transmission
•Data Scan resolution from 5 to 100 m for the 4300A, or 2.5 to
100 m for the 4400A.
•Fast Preview Scan at 40 m
•16-bit ultra-low noise digitization
•Dynamic range of 104
•Easy-access 16-position emission filter cartridge with standard
filters for each pre-installed laser
•Standard 1 inch by 3 inch (25 mm by 75 mm) microscope slides
•Full integration with GenePix Pro Microarray Software and
Acuity®Microarray Software
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Introduction
10 0112-0173 B
Scanner Components
The main components of the GenePix 4300A/4400A Microarray Scanner
are:
•Protective enclosure
•Optics
•Lasers
•Optical Path
•Emission Filters
•PMT (photo-multiplier tube)
•Status lights
.
Figure 1-1 GenePix 4300A/4400A Microarray Scanner
Protective enclosure
The protective enclosure protects the user from exposure to laser
radiation, high voltage, and moving parts.
WARNING! Laser Hazard! Do not remove the protective
enclosure. Operating the scanner with the protective enclosure
removed exposes the user to laser radiation.
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Este manual sirve para los siguientes modelos
1
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