Miltenyi Biotec UltraMicroscope Blaze Manual de usuario

UltraMicroscope Blaze™

User manual

No part of this user manual may be reproduced, stored in a retrieval system, transmitted, published, or distributed in any form or by any means,

electronically, mechanically, by photocopying, microfilming, recording, or otherwise, without the prior written consent of Miltenyi Biotec; however,

notwithstanding the foregoing, the owners of the UltraMicroscope Blaze™ may make copies solely for purposes of training personnel in the use and

servicing of the unit within their business or organization. Maximal care has been taken by Miltenyi Biotec in the preparation of this user manual. However,

Miltenyi Biotec shall not be liable for any technical or editorial errors or omissions contained herein or for incidental or consequential damages in

connection with the furnishing, performance, or use of this document. The information in this document is provided “as is” without warranty of any kind,

and is subject to change without notice. Blaze, MACS, and the Miltenyi Biotec logo are either registered trademarks or trademarks of Miltenyi Biotec and/or

its affiliates in various countries worldwide. All other trademarks mentioned in this document are the property of their respective owners and are used for

identification purposes only. Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic

or diagnostic use.

UltraMicroscope Blaze™

User manual

Original instructions

Issued: 2021-08

212526.01

Miltenyi Biotec B.V. & Co. KG

Friedrich-Ebert-Straße 68

51429 Bergisch Gladbach

Germany

Phone +49 2204 8306-0

Fax +49 2204 85197

macsde@miltenyi.com

www.miltenyibiotec.com

Read the chapter Important safety information and all

other information in this user manual before using the

instrument

Before using the UltraMicroscope Blaze, read the chapter Important safety information and all other

information contained in this user manual, including any safety and operating instructions. Pay special

attention to all warnings displayed on the instrument. Failure to read and follow these guidelines could lead to

improper or incorrect usage and result in damage to the instrument. Improper usage could also cause severe

personal injury, death, unpredictable results, instrument malfunction, and premature wear to components

shortening the lifetime of the instrument. Such actions may void your warranty. Keep the user manual and any

other safety and operating instructions provided with the instrument in a safe place accessible to all users for

future reference.

If you have a serious concern regarding the safe use of your instrument, contact your authorized Miltenyi Biotec

service provider or call Miltenyi Biotec Technical Support.

Content

1 Important safety information 7

2 Introduction 17

2.1 Introduction 17

2.2 Intended use 17

3 Installation 21

3.1 Components 21

3.2 Accessories 22

3.3 Consumables 22

3.4 Unpacking and installation 23

4 Switching on and off 25

4.1 Switching on the instrument 25

4.2 Switching off the instrument 25

4.3 The interface of the UltraMicroscope Blaze 25

5 Preparing the instrument 27

6 Laser alignment 29

6.1 Light sheet alignment 29

6.2 Setting the beam path 29

6.3 Adjustment of the lateral beams on the right-hand side 34

6.4 Adjustment of the center beam on the left-hand side 34

6.5 Adjustment of the lateral beams on the left-hand side 35

7 Setting up an experiment 37

7.1 Capturing a single-color z-stack 37

7.1.1 Aligning the sample 37

7.1.2 Optimizing the measurement settings 39

7.1.3 Capturing an image 40

7.2 Capturing a multi-color z-stack 40

7.3 Capturing a mosaic image 42

8 Maintenance 45

8.1 Servicing 45

8.2 Cleaning the instrument 45

8.3 Cleaning the cuvette 45

9 Technical data and specifications 47

9.1 Specifications 47

9.2 EC / EU Declaration of Conformity 49

10 Technical support 51

11 Legal notes 53

11.1 Limited warranty 53

11.2 Trademarks 54

IMPORTANT SAFETY INFORMATION

Important safety information

General safety instructions

THIS CHAPTER DESCRIBES POTENTIALLY HAZARDOUS SITUATIONS ASSOCIATED WITH THIS INSTRUMENT AND

PROVIDES IMPORTANT SAFETY INFORMATION TO MINIMIZE THE RISKS AND PROTECT YOURSELF AND OTHERS.

The instrument can be used safely, if the following is ensured:

The instrument is installed correctly.

The instrument is only operated by professional laboratory personnel.

General safety practices are followed.

All instructions in this user manual are followed.

• Use this instrument only as indicated in this user manual to avoid injury to personnel and damage of the

equipment.

• Keep this user manual in a place that is always accessible to all users.

• Follow local working area safety instructions and laboratory policies and standards for health, safety, and

prevention of accidents.

• Ensure safe working conditions and provide for sufficient light.

• Ensure that the power switch and the plug of the power cable of the instrument are easily accessible.

• To entirely disconnect the instrument from the power supply, unplug the power cable.

• Ensure that the power cable plug is close to the user.

• If it is necessary to cut the power supply, unplug the cable from the power outlet and contact an authorized

Miltenyi Biotec service provider or Miltenyi Biotec Technical Support.

• Read the instructions in the chapter Important safety information before using the instrument.

• Check the instrument before each use for visible damage.

• In case of severe accidents, damages to the instrument, or if smoke or flames appear, cut the power supply

immediately.

• Do not use the instrument if it has been dropped or is damaged. Contact Miltenyi Biotec Technical Support

immediately.

Hazard levels

Signal words are used to warn against hazardous situations and property damages. The following signal words

are used in this user manual.

or WARNING! indicates a hazardous situation that could result in serious injury or death.

or CAUTION! indicates a hazardous situation that could result in minor or moderate injury. It is also used

to warn against unsafe practices.

or NOTICE indicates information considered important but not hazard related (e.g. messages relating to

property damage).

Safety symbols

SAFETY WARNING: THE DOCUMENTATION MUST BE CONSULTED IN ALL CASES WHERE THIS SAFETY

SYMBOL IS USED ON THE INSTRUMENT

ELECTRICITY

MOVING PARTS

SHARP ELEMENTS

HAZARDOUS LASER RADIATION

BIOLOGICAL HAZARD

CRUSH HAZARD

ERGONOMIC HAZARD

TRIP HAZARD

WEEE (WASTE OF ELECTRICAL AND ELECTRONIC EQUIPMENT)

READ THE USER MANUAL BEFORE USING THE INSTRUMENT

ON (POWER ON)

OFF (POWER OFF)

WEIGHT

SERIAL NUMBER

TYPE NUMBER

ORDER NUMBER

IMPORTANT SAFETY INFORMATION

MANUFACTURER

EUROPEAN CONFORMITY APPROVAL

Safety labels

Notice the hazard points and safety symbols of the instrument.

• Keep safety labels and safety markings clean and legible.

• Inspect the safety labels and safety markings regularly and replace them if they are not legible or identifiable

from a safe viewing distance.

• Contact Miltenyi Biotec for replacement labels.

Figure 1.1: Hazard areas and safety symbols on the front side of the instrument.

Figure 1.2: Hazard areas and safety symbols on the rear side of the instrument.

Figure 1.3: Hazard areas and safety symbols on the left-hand side of the instrument.

Figure 1.4: Hazard areas and safety symbols on the right-hand side of the instrument.

IMPORTANT SAFETY INFORMATION

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