Logos biosystems Luna II Manual de usuario

Catalog No.: L40001 / L40002

Document No.: L44001

Revision Date: December 02, 2014

SW Version: 1.4.4

IMPORTANT

The LUNA-II™ Automated Cell Counter is a Laboratory Electrical Instrument for

Scientific Research Use Only, BUT NOT A MEDICAL or THERAPEUTIC or IN

VITRO DIAGNOTICS DEVICE.

Table of Contents

Safety Information ----------------------------------------------------------------------------------------

General Guidelines --------------------------------------------------------------------------------------

Environment Conditions --------------------------------------------------------------------------------

Chapter 1 –Introduction ----------------------------------------------------------------------

1.1 Product Overview --------------------------------------------------------------------------------

1.2 Key Features --------------------------------------------------------------------------------------

1.3 Product Contents ---------------------------------------------------------------------------------

1.4 Product Specifications --------------------------------------------------------------------------

1.5 Product Description ------------------------------------------------------------------------------

Chapter 2 –Setting up --------------------------------------------------------------------------

2.1 Installation ------------------------------------------------------------------------------------------

2.2 Start-Up Screen -----------------------------------------------------------------------------------

2.3 Settings ---------------------------------------------------------------------------------------------

2.4 Calibrating the Touchscreen (Calibrate Touchscreen) ----------------------------------

2.5 Changing Options for Cell Counting (Counting Options) -------------------------------

2.6 Setting the Date and Time ---------------------------------------------------------------------

2.7 Calibrating LUNA-II™ (Calibrate) ------------------------------------------------------------

2.8 Updating the Software (Update Software) -------------------------------------------------

Chapter 3 –Protocol Setting ----------------------------------------------------------------

3.1 Parameters in the Protocol ---------------------------------------------------------------------

3.2 Setting the Protocols ----------------------------------------------------------------------------

3.3 Selection of Protocol -----------------------------------------------------------------------------

Chapter 4 –Counting Cells ------------------------------------------------------------------

4.1 Preparing the Instrument -----------------------------------------------------------------------

4.2 Sample Preparation ------------------------------------------------------------------------------

4.3 Loading Samples into the Luna™Cell Counting Slide ---------------------------------

4.4 Counting Cells -------------------------------------------------------------------------------------

4.5 After Counting: Image View --------------------------------------------------------------------

4.6 After Counting: Histogram & Gating ---------------------------------------------------------

4.7 After Counting: Saving and Printing the Results ------------------------------------------

4.8 After Counting: Calculation for Subsequent Experiments ------------------------------

Chapter 5 –Focusing Option ---------------------------------------------------------------

5.1 Autofocusing ---------------------------------------------------------------------------------------

5.2 Manual Focusing ---------------------------------------------------------------------------------

Chapter 6 –Review the Previous Results ------------------------------------------

Chapter 7 –Maintenance and Troubleshooting ---------------------------------

7.1 Turn On/Off LUNA-II™ --------------------------------------------------------------------------

7.2 Cleaning --------------------------------------------------------------------------------------------

7.3 Calibrating the Touchscreen -------------------------------------------------------------------

7.4 Calibrating LUNA-II™ ---------------------------------------------------------------------------

7.5 Updating the Software --------------------------------------------------------------------------

7.6 Troubleshooting -----------------------------------------------------------------------------------

Chapter 8 - Ordering Information --------------------------------------------------------

Chapter 9 - Purchaser Notification ------------------------------------------------------

9.1 Limited Use Label License ---------------------------------------------------------------------

9.2 Instrument Warranty -----------------------------------------------------------------------------

Contact Information ------------------------------------------------------------------------------

Safety Information

For best results with the LUNA-II™ Automated Cell Counter, follow the instructions below in addition

to the general precautions for using electrical instruments.

1. Be careful to avoid electric shock while operating the instrument. Do not touch it and other

components with wet hands. Do not place it in a humid environment such as an incubator. For

operating environment, see page 9.

2. Trypan blue stain is known as a hazardous material. While handling the solution, always wear

proper personal protective equipment (PPE) to avoid exposure.

3. Before use, make sure that the input voltage is compatible with the instrument’s power supply

voltage.

4. For optimal operation, place the instrument on a flat surface and avoid any vibration.

5. Turn on the instrument only after connecting both ends of its power cord to the wall outlet as

well as the instrument. Always turn off the instrument before disconnecting the power cord

and/or moving the instrument.

6. Ensure that the power cord is firmly plugged into the power inlet, the wall outlet and AC adapter.

7. When the instrument is operating for a long time, its temperature can become too high. Please

be careful that the instrument’s temperature does not become too high during long and

continuous operation times. When operating, leave enough space around the instrument so

there is enough room for air circulation and cooling.

8. Do not disassemble the instrument in any event. If the instrument is out of order or dropped or

broken, please contact a service person. Disassembling the instrument invalidates its warranty.

9. Use only authorized components (adaptor, power cord, and USB drive).

10. If the instrument emits smoke, disconnect the power cord immediately from the wall outlet and

contact a service person.

11. Used counting slides must be disposed as biohazard wastes.

The WEEE (Waste Electrical and Electronic Equipment) symbol indicates that

users of this instrument have the responsibility of returning and disposing of

WEEE in an ecologically friendly manner. Follow waste ordinances of your region

for proper disposal provisions.

The CE mark indicates that this instrument conforms to all applicable European

Community provisions for which this marking is required. Users must be aware of

and follow the conditions described in this manual for operating the instrument.

The protection provided by the instrument may be impaired if the instrument is

used in a manner not specified by this manual.

Protective earth (Ground)

General Guidelines for Using the LUNA-II™ Automated Cell

Counter

In order to achieve the best results with the LUNA-II™ Automated Cell Counter, follow the

instructions below carefully.

1. The instrument must be operated in compliance with the operating environment described on

page 9. In particular, the temperature and humidity conditions are important.

2. Samples must be handled in an appropriate way, depending on user’s requirements.

3. Hold the Luna™ Cell Counting Slide by the edges to avoid touching its optical surface. Make

sure that no damage or contamination occurs on the optical surfaces of the slide.

4. After mixing the cell sample with trypan blue stain, perform cell counting as soon as possible,

within 3 minutes for accurate cell viability measurement. If needed, count your sample at least

5. Since the LUNA-II™ Automated Cell Counter is calibrated before shipping, you do not need to

re-calibrate before use. However, if re-calibration is needed, please refer to Section 5.3

Calibrating the Counter.

6. Do not touch trypan blue solution with bare hands as it is a hazardous chemical. After using the

counting slides, dispose of it as hazardous wastes. Do not reuse the slides.

Environment Conditions

Operating Power

100 ‒240 VAC, 1.2 A

Frequency

50/60 Hz

Electrical Input

12 VDC, 3.3 A

Installation Site

Indoor use only

Operating Temperature

10 ‒35℃

Maximum Relative Humidity

20 ‒80%

Altitude

≤2,000 m

Pollution Degree

Chapter 1 –Introduction

1.1 Product Overview

The LUNA-II™ Automated Cell Counter is a small, fast, and affordable image-based cell counting

device that automatically counts various kinds of cells for research purpose.

The LUNA-II™ Automated Cell Counter helps measure the number, as well as viability of cells (live,

dead, total cells) with sophisticated optical components and advanced image analysis algorithms.

Due to several innovations introduced by Logos Biosystems, LUNA-II™ provides a state of the art

cell counting device and eliminates the tedium and subjectivity of manual cell counting.

The LUNA-II™ Automated Cell Counter can be used in a very simple procedure. First, mix 10 l of

the cell sample with 10 l of trypan blue stain. Second, load the cell suspension into the Luna™ Cell

Counting Slide. Third, insert the slide into the slide port of the instrument and chose [Autofocused

Counting] or adjust the focus by clicking arrows to get an appropriate cell image. Last, press the

[Count] button and then the results of cell count and viability will be displayed on the screen. The

counting image can be downloaded onto a USB drive in TIF format for review and/or record keeping.

The LUNA-II™ Automated Cell Counter provides key data as below:

Number of live and dead cells/ml

Number of total cells/ml

Viability percentage (% live cells to total cells)

Cell images (showing live cells as green circles and dead cells as red circles)

Histograms of cell size distributions

The Luna™ Cell Counting Slide is disposable and specifically designed for the LUNA-II™

Automated Cell Counter. Each counting slide has 2 chambers, labeled as A and B, respectively, so

that one slide can be used for the same sample reading in duplicate or for 2 different samples if

preferred.

1.2 Key Features

Key features

Description

Small footprint

Compact size with light weight saves space and is suitable for

either laboratory table or biosafety cabinet.

Accuracy & precision

With sophisticated optical components and counting algorithm,

LUNA-II™ provides optimized and reproducible results every

time.

Autofocusing

Advanced liquid lens technology provides fast autofocusing

without mechanical moving. Autofocusing enables reliable cell

counting and excludes human error.

Easy-to-operate user interface

The intuitive user interface based on a touch screen enables

simple and easy operation.

Shortest time-to-results

Results for most cell lines are available within 15 (without

autofocusing) or 22 (with autofocusing) seconds after pressing

the [Count].

Built-in printer (Optional)

An integrated thermal printer provides concise Cell Count

Report facilitating record keeping.

Innovative counting slide

LUNA-II™ adopts an innovative counting slide made with “T-

BOND” technology without using hazardous organic solvents.

Cell concentration & viability range

Measurements can be made for cells at concentrations

ranging from 5 x 104to 1 x 107cells/ml and for cells within the

3 ‒60 m diameter range.

Easy-to-use calculator

No external calculator is needed to calculate subsequent

dilution.

Setup & maintenance

Just plug in and it is ready for use, with virtually no

maintenance costs.

Counting image acquisition

The captured image of cells can be downloaded onto a USB

drive in TIFF (Tag Image File Format) for review or record

keeping.

Individual protocol

Different protocols (up to 300 protocols) can be saved with

personalized parameters.

Documentation

LUNA-II™ provides a PDF (Portable Document Format) report

which includes File name, Date, Cell count results, Cell

images, and histograms.

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