Invitrogen iBright CL1500 Series Manual de usuario
For Research Use Only. Not for use in diagnostic procedures.
iBright™Imaging Systems
USER GUIDE
For use with the iBright™CL1500 Imaging System and the iBright™
FL1500 Imaging System
Catalog Numbers A43678, A44114, A44240 (CL1500) and A43679, A44115, A44241 (FL1500)
Publication Number MAN0018592
Revision A.0
Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL,
INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT,
INCLUDING YOUR USE OF IT.
Revision history: Pub. No. MAN0018592
Revision Date Description
A.0 June 7 2019 New document. Describes installation, operation, and maintenance of the
iBright™Imaging Systems.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept
the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
©2019 Thermo Fisher Scientific Inc. All rights reserved.
Contents
■Product information ....................................................... 6
Product description ............................................................. 6
Contents ....................................................................... 6
■Start, sign on, and configure the instrument ............................ 7
Create an instrument profile ...................................................... 7
Create an instrument account and link to Connect using a cloud account ............... 7
Mobile device ............................................................... 8
PC using Connect account .................................................... 8
PC using instrument Connect app ............................................. 8
Use instrument to link to Connect account ..................................... 8
Link to Connect using an existing instrument account ................................ 9
If the unit is off .................................................................. 9
If the unit is on and in sleep mode ................................................. 9
If the unit is on and in active mode ................................................ 10
■Operate the iBright™imager ............................................ 11
Choose imaging mode and start image capture .................................... 11
Chemi Blots ................................................................... 12
Image using Chemi Blots ................................................... 12
Fluorescent Blots (FL1500 model only) ........................................... 13
Image using Fluorescent Blot ............................................... 13
Nucleic Acid Gels .............................................................. 14
Image using Nucleic Acid Gel ................................................ 14
Protein Gels ................................................................... 15
Image using Protein Gels ................................................... 15
Universal Mode ................................................................ 16
Options available in modes ...................................................... 18
Image Gallery ................................................................. 21
View images .............................................................. 21
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■Export and analyze images .............................................. 22
Export images ................................................................. 22
Export new images ........................................................ 22
Export gallery images ...................................................... 22
Select an Export file type ................................................... 23
Analyze images ................................................................ 24
Analyze new images ....................................................... 24
Analyze gallery images ..................................................... 25
■Maintain the instrument ................................................. 27
Settings ....................................................................... 27
Manage accounts .......................................................... 29
Run instrument diagnostics ..................................................... 29
Clean the instrument ........................................................... 30
Replace the instrument fuses .................................................... 30
Materials required ......................................................... 30
Replace the fuses .......................................................... 30
Move the instrument ........................................................... 31
Prepare for shipping ....................................................... 31
■Instrument specifications ............................................... 33
Instrument dimensions and specifications ......................................... 33
Electrical requirements ......................................................... 33
Environmental requirements .................................................... 33
Network requirements .......................................................... 34
Instrument and computer connections ............................................ 35
■Safety ...................................................................... 37
Symbols on this instrument ..................................................... 37
Safety alerts on this instrument ............................................. 38
Location of safety labels on the instrument ................................... 39
Safety information for instruments not manufactured by Thermo Fisher Scientific ..... 39
Instrument safety .............................................................. 39
General .................................................................. 39
Physical injury ............................................................ 39
LED (Light-Emitting Diode) .................................................. 40
Electrical safety ........................................................... 40
Cleaning and decontamination ............................................... 40
Instrument component and accessory disposal ................................ 41
Contents
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Safety and electromagnetic compatibility (EMC) standards .......................... 42
Safety compliance ......................................................... 42
EMC ...................................................................... 42
Environmental design ...................................................... 43
Chemical safety ................................................................ 44
Biological hazard safety ......................................................... 45
■Documentation and support ............................................. 46
Related documentation ......................................................... 46
Customer and technical support ................................................. 46
Limited product warranty ....................................................... 46
Contents
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Imaging Systems User Guide
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Product information
IMPORTANT! Before using this product, read and understand the information in the
“Safety” appendix in this document.
Product description
The iBright™ CL1500 Imaging System allows users to image chemiluminescent
Western blots, DNA and RNA gels stained with uorescent nucleic acid stains, and
visible protein gels. The iBright™ FL1500 Imaging System adds the capability of
imaging uorescent Western blots using multiple dyes.
The iBright™ imagers use a simple design and intuitive workows to deliver high-
resolution images. They can be run directly from the touchscreen to start and create
images, while also providing on-board software for image analysis. To further analyze
data, the instrument can be integrated with the iBright™ Image Analysis Software
available for desktop and Connect.
Contents
Table 1 Included imager system parts
Component Quantity
Power cord (region specific) 1
Quick reference card 1
White transilluminator screen 1
iBright™Imaging System Sample Blot 1
Sample stage/turntable 1
Table 2 Available accessories
Component Part number
White transilluminator screen A33828
Sample stage A33829
High-Power USB Wi-Fi Module A26774
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Start, sign on, and configure the
instrument
Create an instrument profile
1. Touch to sign in to your instrument user account.
2. Touch Get started.
3. Touch Create a Prole.
4. Enter a Screen name.
5. Enter and conrm a four-digit pin.
6. Touch Create prole.
7. (Optional) If you want to link your instrument to your Connect account, see “Use
instrument to link to Connect account“ on page 8 or “Link to Connect using an
existing instrument account“ on page 9.
Create an instrument account and link to Connect using a cloud
account
1. Touch to sign in to your instrument user account.
2. Touch Get Started.
3. Click on Connect.
4. Link your account using one of these methods:
•Mobile device — Connect using a QR generated by the instrument and
scanned by the instrument Connect mobile app.
•PC — Connect using a link code that is entered into the instrument Connect
app or your online Connect account.
•Instrument — Enter your Connect account information directly on the
instrument.
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1. Touch Mobile Device.
2. Launch instrument Connect mobile app.
3. Sign into your Connect account on the mobile app.
4. Click on .
5. Click on QR code.
6. Scan QR code on the instrument.
Your Connect account should now be linked to your instrument account.
1. Touch PC.
2. Login to your Connect account online.
3. Click on
.
4. Click on .
5. Select iBright from the drop-down menu.
6. Enter the linking code and click on Send.
Your Connect account should now be linked to your instrument account.
1. Touch PC.
2. Launch the instrument Connect mobile app.
3. Sign into your Connect account on the mobile app.
4. Click on .
5. Click on Linking code.
6. Enter the linking code and click Send.
Your Connect account should now be linked to your instrument account.
1. Click on Instrument.
2. Enter your Connect username and password.
3. Click Link account.
Your Connect account should now be linked to your instrument account.
Mobile device
PC using Connect
account
PC using
instrument
Connect app
Use instrument to
link to Connect
account
Start, sign on, and configure the instrument
Create an instrument account and link to Connect using a cloud account
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Link to Connect using an existing instrument account
Linking to Connect after account creation can only be done during the export process.
1. Click on .
2. Select an image.
3. Click on Actions.
4. Click on Export.
5. Click on Choose destination.
6. Click on Cloud.
7. Click on Sign in.
8. Connect using one of the methods outlined in “Create an instrument account and
link to Connect using a cloud account“ on page 7.
If the unit is off
1. Ensure the unit is plugged in and turn on using the switch on the back of
instrument.
The instrument will go directly to the sign-in page.
2. Enter a Screen name.
3. Enter and conrm a 4-digit pin.
Note: For those not logged on as a user (guest), acquired images are saved to a
guest gallery. The guest gallery is not private. Any logged in user can view and
modify images residing in the guest gallery.
4. From the Welcome screen, choose the appropriate Mode from the drop-down
menu, touch and place sample in the center of the transilluminator glass.
5. Touch to close drawer (do not physically push on the drawer). Instrument
adjusts and displays a live-view sample image in the viewport.
If the unit is on and in sleep mode
1. Touch the screen to wake.
2. Touch to sign in to use or set up a user prole. See “Create an instrument
prole“ on page 7 for instructions on how to create a user prole.
Note: For those not logged on as a user (guest), acquired images are saved to a
guest gallery. The guest gallery is not private. Any logged-in user or other guest
user can view and modify images residing in the guest gallery.
Start, sign on, and configure the instrument
Link to Connect using an existing instrument account
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3. Enter a Screen name.
4. Enter and conrm a 4-digit pin.
5. From the Welcome screen, choose the appropriate Mode from the drop-down
menu, touch Open Drawer, and place sample in the center of the transilluminator
glass.
6. Touch to close drawer (do not physically push the drawer). Instrument
adjusts and displays a live-view sample image in the viewport.
If the unit is on and in active mode
1. If not already signed in, touch to sign in or set up a new user prole. See
“Create an instrument prole“ on page 7 for instructions on how to create a user
prole.
Note: For those not logged on as a user (guest), acquired images are saved to a
guest gallery. The guest gallery is not private. Any logged-in user can view and
modify images residing in the guest gallery.
2. From the Welcome screen, choose the appropriate Mode from the drop-down
menu, touch and place sample in the center of the turntable glass.
3. Touch to close drawer (do not physically push the drawer). Instrument
adjusts and displays a live-view sample image in the viewport.
Start, sign on, and configure the instrument
If the unit is on and in active mode
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Imaging Systems User Guide
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1
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