Labfors 5 – Version for Solid Substrates and Enzymatic Bioprocesses
Table of Contents
Page 4of 180 22 January 2019
8.1.4 Moistening/Filling the Culture Vessel ...........92
8.1.5 Fitting the Vessel Top Plate..........................93
8.1.6 Mounting a Threaded Adapter......................94
8.1.7 Mounting the Blanking Plugs ........................95
8.1.8 Mounting Addition Port Adapters..................97
8.1.9 Mounting the Pocket for Temperature Sensor
(Pt100) ..........................................................98
8.1.10 Preparing an Inoculation Needle ..................98
8.1.11 Equipping Port with Septum Collar and
Septum for Inoculation..................................99
8.1.12 Mounting and Preparing the Exit Gas Cooler
......................................................................99
8.1.13 Checking Lubrication of the Mechanical Seal
....................................................................101
8.1.14 Preparing the Sensors................................101
8.1.14.1 Calibrating the pH Sensor .......... 102
8.1.14.2 Fitting a pH Sensor with a Probe
Protection ................................... 103
8.1.14.3 Mounting Sensors with Electrode
Holder......................................... 103
8.1.15 Preparing the Super Safe Sampler.............105
8.1.16 Mounting the Sparger Hose and the Inlet Air
Filter ............................................................107
8.1.17 Mounting the Hose and Inlet Air Filter for
Head Space Gassing..................................107
8.1.18 Preparing the Gassing Hose Line on the Basic
Unit .............................................................108
8.1.19 Calibrating the Pumps ................................109
8.1.20 Preparing the Reagent Bottles, Pumps and
Hoses..........................................................109
8.1.21 Sterile Hose Connections ...........................112
8.1.22 Removing the Pump Heads........................112
8.1.23 Checklist Before Autoclaving......................113
8.1.24 Autoclaving .................................................115
8.2 Connecting the Culture Vessel and Preparing the
Cultivation ..................................................................116
8.2.1 Connecting the Culture Vessel ...................116
8.2.2 Mounting the Pump Heads .........................117
8.2.3 Filling the Reagent Hoses ..........................117
8.2.4 Connecting the Gassing .............................118
8.2.5 Connecting the Exit Gas Cooler .................119
8.2.6 Coupling the Motor .....................................120
8.2.7 Filling the Culture Vessel............................120
8.2.8 Inserting the Temperature Sensor (Pt100) into
the Pocket...................................................121
