ibidi Heating System Manual de usuario
Version 25.04.2017
Heating System
Instructions
Instructions
2 Heating System Version (26th April 2017)
Instructions
Contents
Safety Considerations 4
1 Principle – Physiological Conditions in Live Cell Imaging 15
2 Equipment 16
2.1 Components of the Heating System ............................. 16
2.2 Combination Options ...................................... 17
2.3 Temperature Controller ..................................... 18
2.4 Heated Lid ............................................ 20
2.5 Heated Plate ........................................... 20
2.6 Heating Inserts ......................................... 21
2.6.1 Heating Insert for µ–Slides .............................. 21
2.6.2 Heating Insert for µ–Dishes .............................. 22
3 Operating the Heating System 23
3.1 Installation and Connection of the Parts ........................... 23
3.2 Sample Preparation ....................................... 23
3.3 Start the Heating System .................................... 24
3.4 Setting Temperatures in the Control Panel .......................... 24
3.5 Temperature Adjustment in the Sample ........................... 25
4 Maintenance 27
4.1 Sterilization and Cleaning ................................... 27
4.2 Influence of Surrounding Temperature and Airflow .................... 27
5 Troubleshooting 28
5.1 Focus not Stable ......................................... 28
5.2 Evaporation is Too High .................................... 28
5.2.1 Silicone Oil Procedure (Anti-Evaporation Oil) ................... 28
Version (26th April 2017) Heating System 3
Instructions
Safety Considerations
To ensure operation safety, the Heating System must be operated correctly and maintained accord-
ing to a regular schedule. Carefully read all safety precautions in this manual before operating the
instrument. Note that the signal words WARNING,CAUTION and NOTE have specific meanings
in this manual.
Safety Symbols
Do not proceed beyond a WARNING or CAUTION notice until you have performed the indicated
actions.
WARNING! A potentially hazardous situation which, if not avoided, could result in
serious injury or even death.
CAUTION A potentially hazardous situation which, if not avoided, could result
in minor or moderate injury. It is also used to alert against damaging
the equipment or the instrument.
NOTE Additional information to help achieve optimal instrument and assay
performance.
This label is positioned on the back of the device and prompts you to
read the manual before using the device.
Warning message
Warning messages in the text are displayed in a gray shaded box.
4 Heating System Version (26th April 2017)
Instructions
Nomenclature
Temperature Controller
Heated Lid
Heated Plate
WARNING!
• Do only operate the Heating Devices with the supplied Temperature Controller.
• Do only use the delivered cables and plugs. If not doing so you risk electric shock and fire.
• Do not operate the Heating System with substances or under conditions, that can cause a risk
of explosion, implosion or the release of gases.
CAUTION
• Ensure that the external power supply is well accessible. The Heating System must be installed
in such manner that none of its components hinders access to the external power supply.
• The weight of the Temperature Controller is approx. 3 kg. Moving the Temperature Controller
during operation will pose a risk of personal injury or damage to the instrument.
• Some accessible parts of the Heated Plate and Heated Lid can reach temperatures up to 55°C.
Avoid touching the temperature-controlled parts of the system when you have set the Temper-
ature Controller to high temperatures.
• The glass plate of the Heated Lid can break due to a mechanical impact. If so, the shards can
lead to injuries if handled.
• Be aware that when the system is switched on, 10 V AC voltage is applied to the underside of
the glass on the Heated Lid and bottom of the Heated Plate. Do not touch the underside or put
it in contact with anything conductive. This could cause a short circuit that may destroy the
Temperature Controller and/or the Heating Devices.
• Only use the Heating System in dry rooms.
• Do not use the Heating System in cold rooms.
• Use the Heating System only for cell experiments with aqueous solutions.
• Do not use the Heating System with hazardous substances or substances/materials that pose a
risk of infections.
• Do not use extension cords. Immediately replace damaged cords, plugs, or cables to avoid a
risk of personal injury or damage to the instrument.
• Only ibidi technical staff is permitted to open and service the Heating Devices and/or Temper-
ature Controller.
• Unplug the external power supply when the Heating System is not in use.
Version (26th April 2017) Heating System 5
Instructions
Regulatory Statement
The following safety and electromagnetic standards were con-
sidered:
• IEC 61010-1:2010 + Cor. :2011 Safety requirements for
electrical equipment for measurement, control and labo-
ratory use - Part 1: General requirements
• IEC 61326-1:2006 EMC, Electrical equipment for measure-
ment, control and laboratory use. EMC requirements.
General requirements
• IEC/CISPR 11:2009, modified + A1:2010, Industrial, sci-
entific and medical equipment - Radio-frequency distur-
bance characteristics - Limits and methods of measure-
ment
6 Heating System Version (26th April 2017)
Instructions
Specifications
External Power Supply:
Heated Lid: AC 10 V, 50-60 Hz
Heated Plate: AC 24 V, 50-60 Hz
Temperature Controller: AC 230 V/50 Hz or 115 V/60 Hz
Operating Conditions
Environmental operating temperature: 18-30°C (indoor use only)
Storage temperature: -5-50°C, humidity up to 80% RH
Humidity: 80% RH up to 30°C
Operating altitude: max. 2000 m (atmospheric pressure 800-1060 hPa)
Dimensions of Heated Plate with Lid
Width: 125.5 mm
Depth: 85.5 mm
Height: 25.5 mm
Weight: 210 g
Temperature Control Range
Heated Lid (Channel 1): Ambient temperature (min. 18°C) to +46°C
Heated Plate (Channel 2): Ambient temperature (min. 18°C) to +45°C
Recommended Temperatures before Adjustment
(see Section3.5)
Heated Lid (Channel 1): 42°C
Heated Plate (Channel 2): 37°C
Version (26th April 2017) Heating System 7
Instructions
Compatibility
The parts of the Heating System must only be used according to the instructions. The compatibility
of all parts is shown in detail in section 2.2.
Notices
1. ibidi shall not be held liable, either directly or indirectly, for any consequential damage incurred
as a result of product use.
2. Prohibitions on the use of ibidi software
• Copying software for other than backup purposes
• Transfering or licensing of the right to use software to a third party
• Disclosure of confidential information regarding software
• Modification of software
• Use of software on multiple workstations, network terminals, or by other methods
3. The contents of this manual are subject to change without notice for product improvement.
4. This manual is considered complete and accurate at publication.
5. This manual does not guarantee the validity of any patent rights or other rights.
6. If an ibidi software program has failed causing an error or improper operation, this may be
caused by a conflict from another program operating on the notebook (PC). In this case, take
corrective action by uninstalling the conflicting product(s).
7. ibidi is a registered trademark of ibidi GmbH in Germany and other countries.
8 Heating System Version (26th April 2017)
Instructions
Limited Warranty
Products sold by ibidi, unless otherwise specified, are warranted for a period of one year from the
date of shipment to be free of defects in materials and workmanship. If any defects in the product are
found during this warranty period, ibidi will repair or replace the defective part(s) or product free of
charge.
This warranty does not apply to defects resulting from the following:
1. Improper or inadequate installation.
2. Improper or inadequate operation, maintenance, adjustment or calibration.
3. Unauthorized modification or misuse.
4. Use of unauthorized tubing or fluidic connectors.
5. Use of consumables, disposables and parts not supplied by an authorized ibidi distribu-
tor.
6. Corrosion due to the use of improper solvents, samples, or due to surrounding gases.
7. Accidents beyond ibidi’s control, including natural disasters.
This warranty does not cover consumables, such as cell culture chambers and dishes, tubes, fluidic
connectors, reagents etc.
The warranty for all parts supplied and repairs provided under this warranty expires on the warranty
expiration date of the original product. For inquiries concerning repair service, contact ibidi and
provide the model name and serial number of your ibidi System.
Version (26th April 2017) Heating System 9
Instructions
Installation Requirements
To ensure operation safety and optimal performance, meet the following conditions:
1. Only operate the ibidi Heating Devices with an ibidi Temperature Controller and with the sup-
plied power cable.
2. Ensure that the power plug of the power supply is well accessible. The Heating Devices must
be installed in a manner that they do not hinder the access to the external power supply and its
power plug.
3. Only operate the Heated Plate with original ibidi inserts.
4. Operate the Heating System in a temperature range of 18-30°C.
5. Operate the Heating System in a humidity range of up to 80% RH.
6. Operate the Heating System in an atmospheric pressure range of 800-1060 hPa.
7. Do not operate the Heating System under conditions that pose a risk of explosion, implosion,
or the release of gases.
8. Avoid strong magnetic fields and sources of high frequency. The Heating System might not
function properly when located near a strong magnetic field or high frequency source.
9. Avoid vibrations from vacuum pumps, centrifuges, electric motors, processing equipment, and
machine tools.
10. Avoid dust and corrosive gas. Do not install the Heating System where it could be exposed to
high levels of dust or to outside air or ventilation outlets.
11. Do not install the Heating System in a location where it could be exposed to direct sunlight.
12. Install the Heating System in a horizontal and stable position, which includes a table, bench or
desk upon which the instrument is installed.
13. Install the Heating System in a location that enables easy access for maintenance.
14. Do not place heavy objects on the instrument.
10 Heating System Version (26th April 2017)
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