HEKA EPC 10 USB Manual de usuario

EPC 10 USB

USER MANUAL

EPC 10 USB Manual, Version 3.0

HEKA Elektronik GmbH Phone +49 7121 90925 26

Aspenhaustrasse 21 Fax +49 7121 90925 11

D-72770 Reutlingen Website www.heka.com /www.elproscan.com

Germany Email eimeaorderscmt@harvardbioscience.com

[email protected]

HEKA Electronic Phone +1 800 597 0580

84 October Hill Road Fax +1 508 429 5732

01746 Holliston Website www.heka.com /www.elproscan.com

Massachusetts Email [email protected]

United States [email protected]

EPC 10 USB • March 2022 • www.heka.com

TABLE OF CONTENTS

Table of Contents

Table of Contents iv

List of Figures vi

List of Tables vii

1 General Information 1

1.1 ImportantSafetyAdvice............................................ 1

2 Introduction 5

2.1 IntroducingtheEPC10USB.......................................... 5

2.2 References .................................................... 6

2.3 NamingConventions.............................................. 7

2.4 MSWindowsversions ............................................. 7

2.5 Macintosh .................................................... 7

3 Description of the Hardware 9

3.1 Headstage .................................................... 9

3.2 HDMIRedStarHeadstage........................................... 9

3.3 MainUnit .................................................... 10

3.3.1 Front-panel ............................................... 10

3.3.2 Rear-panel................................................ 12

3.4 EPC 10 USB DOUBLE, TRIPLE, and QUADRO . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.5 SpecialFeaturesoftheEPC10USB...................................... 13

3.5.1 Timing of the AD-/DA-channels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4 Installation 15

4.1 InstallingSoftwareonly ............................................ 15

4.2 Installing Hardware and Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

4.3 Conventions................................................... 15

4.4 ConnectingtheHardware ........................................... 16

4.5 Protection Key (’Dongle’) Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

iEPC 10 USB • March 2022 • www.heka.com

TABLE OF CONTENTS

4.5.1 USBDongleInstallation........................................ 17

4.5.1.1 MSWindows......................................... 17

4.5.1.2 MacOS ............................................ 17

4.6 SoftwareInstallation .............................................. 17

4.6.1 MSWindows .............................................. 17

4.6.2 MacOS.................................................. 18

4.7 CalibratingtheHardware ........................................... 18

4.8 SupportHotline................................................. 19

5 Ampliﬁer Window 21

5.1 GlobalSection.................................................. 22

5.1.1 Tweak .................................................. 23

5.1.1.1 ViewTab ........................................... 23

5.1.1.2 HardwareTab ........................................ 24

5.1.1.3 ToolsTab ........................................... 26

5.1.1.4 CompensationTab...................................... 28

5.2 AmpliﬁerSelection ............................................... 30

5.3 AmpliﬁerParameters.............................................. 31

6 Verifying and Testing the EPC 10 USB 37

6.1 Testing the EPC 10 USB with the Model Circuit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

6.1.1 TheModelCircuit ........................................... 37

6.1.2 "SETUP" - Applying the Test Pulse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

6.1.3 "SEAL" - Voltage Clamp Recording . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

6.1.4 "Whole-Cell" - Voltage Clamp Recording . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

6.1.5 "Whole-Cell" - Current Clamp Recording . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

6.1.6 Measuring the Noise of the Ampliﬁer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

6.2 MakingaFullTest................................................ 48

7 Operating Modes 51

7.1 VoltageClampMode.............................................. 51

7.2 CurrentClampMode.............................................. 51

7.3 Low Frequency Voltage Clamp Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

8 Compensation Procedures 55

8.1 SeriesResistanceCompensation........................................ 55

8.2 CapacitanceCompensation .......................................... 57

8.3 OffsetCompensation.............................................. 58

9 Patch Clamp Setup 61

EPC 10 USB • March 2022 • www.heka.com ii

TABLE OF CONTENTS

9.1 MountingtheProbe............................................... 61

9.2 GroundWires .................................................. 61

9.3 GroundingtheMicroscope........................................... 61

9.4 ExternalShielding................................................ 62

9.5 ConnectionstootherInstruments....................................... 62

9.6 PipetteHolderandElectrode ......................................... 62

9.7 BathElectrode.................................................. 63

10 Patch Pipettes 65

10.1GlassCapillaries................................................. 65

10.2Pulling ...................................................... 65

10.3Coating...................................................... 65

10.4HeatPolishing.................................................. 66

10.5UseofPipettes.................................................. 66

11 Using the Patch Clamp 67

11.1FormingaSeal.................................................. 67

11.1.1 InitialSetup............................................... 67

11.1.2 EnteringtheBath............................................ 67

11.1.3 FormingaGigaseal........................................... 67

11.2Cell-AttachedRecording............................................ 68

11.3Whole-CellRecording ............................................. 68

11.3.1 BreakingthePatch ........................................... 68

11.3.2 Capacitive Transient Cancellation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

11.3.3 Series Resistance Compensation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

11.4CurrentClampRecording ........................................... 70

12 Low Noise Recording 71

13 Appendix I: Digital Inputs/Outputs 73

13.1DigitalI/OConnector ............................................. 73

13.2DigitalInConnector .............................................. 74

13.3DigitalOutConnector ............................................. 74

13.4BreakoutCables................................................. 75

13.5ConnectingCables ............................................... 76

13.5.1 DG 4/5 / Lambda 10-2 / Lambda 10-3 to EPC 10 USB . . . . . . . . . . . . . . . . . . . . . . 76

13.5.2 ValveLink8toEPC10USB ...................................... 78

14 Appendix II: Probe Adapters 79

14.1StandardMountingPlate............................................ 79

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TABLE OF CONTENTS

14.2DovetailMountingPlate............................................ 79

15 Appendix III: S-Probe 81

16 Appendix IV: Pipette Holder 83

16.1BNC-Type .................................................... 83

16.2SMA-Type .................................................... 84

EPC 10 USB • March 2022 • www.heka.com iv

LIST OF FIGURES

List of Figures

2.1 EPC10USBPatchClampampliﬁer ..................................... 5

3.1 EPC 10 USB HDMI Red Star Headstage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

3.2 EPC10USBFrontPanel............................................ 10

3.3 EPC10USBRearPanel ............................................ 12

3.4 Double Staggered Acquisition Mode of the DAQ board. . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.1 Exemplary start dialog of the calibration process. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

4.2 Prompting dialog for shielding the probe inputs. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

4.3 Prompting dialog for connecting the model cell. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

5.1 Default Settings of the Ampliﬁer Window. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

5.2 The three sections of the Ampliﬁer Window. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

5.3 Global Section of the Ampliﬁer Window. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

5.4 Tweak extension of the Ampliﬁer Window. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

5.5 ViewtaboftheAmpliﬁerWindow....................................... 23

5.6 Hardware tab of the Ampliﬁer Window. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

5.7 ToolstaboftheAmpliﬁerWindow....................................... 27

5.8 Ampliﬁer Info dialog of the Tweak menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

5.9 Compensation tab of the Ampliﬁer Window. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

5.10 Ampliﬁer Selection of an EPC 10 USB Double ampliﬁer. . . . . . . . . . . . . . . . . . . . . . . . . . 30

5.11 Ampliﬁer Parameters of an EPC 10 USB ampliﬁer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

5.12 Bridge Compensation Options in Current Clamp Mode. . . . . . . . . . . . . . . . . . . . . . . . . . . 35

6.1 ModelCircuitoftheMC-10 .......................................... 37

6.2 EPC 10 USB Double Ampliﬁer and Test Pulse window. . . . . . . . . . . . . . . . . . . . . . . . . . . 38

6.3 BeforeC-Fastcompensation.......................................... 40

6.4 AfterC-Fastcompensation........................................... 41

6.5 BeforeC-Slowcompensation ......................................... 42

6.6 AfterC-Slowcompensation .......................................... 43

6.7 CurrentClampMode.............................................. 45

vEPC 10 USB • March 2022 • www.heka.com

LIST OF FIGURES

6.8 Noisemeasurement............................................... 47

6.9 The Full Test is selectable in the Tools selection of the Ampliﬁer Window. . . . . . . . . . . . . . . . . 48

7.1 Circuitry of the Voltage Clamp mode of the EPC 10 USB. . . . . . . . . . . . . . . . . . . . . . . . . . 51

7.2 Circuitry of the Current Clamp mode of the EPC 10 USB. . . . . . . . . . . . . . . . . . . . . . . . . 52

8.1 Series Resistance Compensation Circuit of the EPC 10 USB. . . . . . . . . . . . . . . . . . . . . . . . 56

9.1 Bathelectrode .................................................. 63

13.1 Digital I/O connector (40-Pin, male) of the EPC 10 USB . . . . . . . . . . . . . . . . . . . . . . . . . . 74

13.2 Digital In connector (DSub-25, female) of the EPC 10 USB . . . . . . . . . . . . . . . . . . . . . . . . . 74

13.3 Digital Out connector (DSub-25, female) of the EPC 10 USB. . . . . . . . . . . . . . . . . . . . . . . . 75

13.4 DSub-25, female, to 8x BNC breakout cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75

13.5 DSub-25 (male) to DSub-25 (female) connecting cable . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

13.6 DSub-25 (male) to DSub-9 (male) connecting cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78

14.1 Standard Mounting Plate for EPC 10 USB pre-ampliﬁers. . . . . . . . . . . . . . . . . . . . . . . . . . 79

14.2 Dovetail Mounting Plate for EPC 10 USB pre-ampliﬁers. . . . . . . . . . . . . . . . . . . . . . . . . . 79

15.1 S-Probe Headstage with HDMI cable. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

15.2 Adapter for connecting the model circuit to the S-Probe . . . . . . . . . . . . . . . . . . . . . . . . . . 81

16.1 BNC-type Pipette Holder for the Red Star Headstage . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

16.2 Pipette Holder SMA-type for the S-Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

EPC 10 USB • March 2022 • www.heka.com vi

LIST OF TABLES

List of Tables

3.1 Number of free DA/AD channels in EPC 10 USB Multi ampliﬁers. . . . . . . . . . . . . . . . . . . . 13

5.1 Current Gain ranges of the EPC 10 USB ampliﬁer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

5.2 Filter 1 setting according to the deﬁned Series Resistance Compensation speed . . . . . . . . . . . . 36

8.1 Maximum RS-compensation values. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57

8.2 Exemplary solutions and their Liquid Junction Potentials. . . . . . . . . . . . . . . . . . . . . . . . . . 59

12.1 Noise Contribution of Different Hardware Components. . . . . . . . . . . . . . . . . . . . . . . . . . 71

13.1 Pin assignment of the Digital I/O Connector (EPC 10 USB). . . . . . . . . . . . . . . . . . . . . . . . . 73

13.2 Pin assignment of the Digital In Connector (EPC 10 USB), upper row. . . . . . . . . . . . . . . . . . . 74

13.3 Pin assignment of the Digital In Connector (EPC 10 USB), lower row. . . . . . . . . . . . . . . . . . . 74

13.4 Pin assignment of the Digital Out Connector (EPC 10 USB), upper row. . . . . . . . . . . . . . . . . . 74

13.5 Pin assignment of the Digital Out Connector (EPC 10 USB), lower row. . . . . . . . . . . . . . . . . . 75

13.6 Pin assignments of Digital Out Connector of EPC 10 USB and Parallel Port connector of DG 4/5. . . 76

13.7 Pin assignments of Digital Out Connector of EPC 10 USB and Parallel Port connector of Lambda 10-2. 77

13.8 Pin assignments of Digital Out Connector of EPC 10 USB and Parallel Port connector of Lambda 10-3. 77

13.9 Pin assignments of Digital Out Connector of EPC 10 USB and connector of ValveLink 8. . . . . . . . 78

vii EPC 10 USB • March 2022 • www.heka.com

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