Harvard Bioscience Biochrom Libra S6+ Manual de usuario

5061-071 Rev 1.0

Libra S6+

User Manual

Libra S6+80-5001-20

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Table of Contents

Essential Safety Notes .....................................................................................................................................................4

Unpacking, Positioning, Installation ..............................................................................................................................4

Warranty and Repair ........................................................................................................................................................5

Technical Specifications ……………………………………………………………………………………………………………….6

Operation..……………………………………………………………………………………………………………………………….7

Introduction ……………….…………………………………………………………………………………………………………7

Sample Handling Tips ..……………………………………….……………………………………………………………………7

Keypad and Display ..…………….…………………………………………………………………………………………………8

Software Style …………..……………………………………..……………………………………………………………………9

Applications Folder…………………………………………………………………………………………………………………….10

Applications…………………………………………………………………………………………………………………………11

Single Wavelength…………………………………………………………………………………………...………………11

Concentration…………………………………………………………………………………………………………………13

Wavescan…..………………………………………………………………………………………………………………...16

Simple Kinetics……………………………………………………………………………………………………………….19

Standard Curve……………………………………………………………………………………………………………….22

Multiple Wavelength………………………………………………………………………………………………………….29

Absorbance Ratio…………………………………………………………………………………………………………….31

The Life Science Folder……………………………………………………………………………………………………………….34

Applications (Protein Determination).……………………………………………………………………………………...........35

BCA………………………………………………………………………………………………………………………...…36

Bradford……………………………………………………………………………………………………………………….41

Lowry…………………………………………………………………………………………………………………………..46

Biuret…………………………………………………………………………………………………………………………..51

OD600…………………………………………………………………………………………………………………………55

Methods and Favourties Folder …………………………………………………………………………………………………….57

Saving a Method …………………………………………………………………………………………………………………..57

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Delete a Method …………………………………………………………………………………………………………………..58

Lock Method………………………………………………………………………………………………………………………..58

Unlock Method……………………………………………………………………………………………………………………..59

Favourites…………………………………………………………………………………………………………………………..60

Saving Data onto a USB Memory Stick ………………………………………………………………………………………...61

Utilities Folder ………………………………………………………………………………………………………….……………..63

Date and Time …………………………………………………………………………………………………………………….64

Regional …………………………………………………………………………………………………………………………...64

Export Data ………………………………………………………………………………………………………………………..65

Preference …………………………….…………………………………………………………………………………………..65

Contrast ……………………………………………………………………………………………………………………………66

About ……………………………………………………………………………………………………………………………….66

Print Via Computer ……………………………………………………………………………………………………………………67

Installation ………………………………………………………………………………………………………………………….67

Test Tube and Heated Cell Holder Installation …………………………………………………………………………………….68

Accessories ……………………………………………………………………………………………………………………………69

Cleaning and General Care of the Instrument ……………………………………………………………………………………..69

External cleaning ………………………………………………………………………………………………………………….69

Changing cell holder or removal for cleaning …………………………………………………………………………………..69

Icon Glossary ………………………………………………………………………………………………………………………….70

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Essential Safety Notes

There are a number of warning labels and symbols on your instrument. These are there to inform you where potential

danger exists or particular caution is required. Before commencing installation, please take time to familiarize yourself

with these symbols and their meaning.

Caution (refer to accompanying documents).

Unpacking, Positioning and Installation

Check the contents of the package against the packing list. If any shortages are discovered, inform your supplier

immediately.

Inspect the instrument for any signs of damage caused in transit. If any damage is discovered, inform your supplier

immediately.

Ensure your proposed installation site conforms to the environmental conditions for safe operation:

Indoor use only.

Temperature range 5°C to 35°C. Note that if you use the instrument in a room subjected to extremes of

temperature change during the day, it may be necessary to recalibrate (by switching off and then on again).

Maximum relative humidity of 80% up to 31°C decreasing linearly to 50% at 40°C.

The instrument must be placed on a stable, level bench or table that can take its weight (1.55 kg) to allow air to

circulate freely around the instrument.

This equipment must be connected to the power supply with the power cord supplied. It can be used on 90 – 240 V~,

50-60 Hz supplies.

If the instrument has just been unpacked or has been stored in a cold environment, it should be allowed to come to

thermal equilibrium for 2-3 hours in the laboratory before turning the instrument on. This will prevent calibration failure

as a result of internal condensation.

Switch on the instrument via the keypad ( ) after it has been plugged in. The instrument will perform a series of

self-diagnostic checks.

Please read through this user manual prior to use.

Please contact your original supplier in the first instance if you experience technical or sample handling difficulties.

If this equipment is used in a manner not specified or in environmental conditions not appropriate for safe operation, the

protection provided by the equipment may be impaired and instrument warranty withdrawn.

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Warranty and Repair

Biochrom warrants this instrument for a period of 12 months (1 year) from the date of purchase. Where appropriate,

Biochrom will repair or replace the unit for defects of workmanship or materials. This warranty does not extend to damage

resulting from misuse, neglect, or abuse, normal wear and tear, or accidental damage. This warranty extends only to the

original consumer purchaser.

Products failing within the first 30 days of end user operation are considered dead on arrival (DOA) and where appropriate

a replacement will be given if a repair is not possible. In the instance of a DOA; Biochrom will incur shipping charges of

returning the unit back to the facility.

IN NO EVENT SHALL BIOCHROM BE LIABLE FOR INCIDENTAL OR CONSEQUENTAL DAMAGES. Some states do

not allow the exclusion or limitation of incidental or consequential damages do the above limitation to exclusion may not

apply to you. THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR USE,

OR OF ANY OTHER NATURE. Some states do not allow this limitation on an implied warranty, so the above limitation

may not apply to you.

If any defect arises within or outside the warranty period, please contact:

US Office Technical Support

Email support@hbiosci.com

Telephone (Toll Free) 1-800-272-2775

Telephone (Outside the US) 1-508-893-8999

Address 84 October Hill Road

Holliston MA 01746

USA

Office Technical Support

Email support@hbiosci.com

Telephone +44 (0) 1223-423-723

Address 1020 Cambourne Business Park

Cambourne

Cambridge UK

CB23 6DW

Goods will not be accepted for return unless a RMA (Returns Materials Authorization) number has been issued by our

Returns-Repairs Department. The customer is responsible for shipping charges. Please allow a reasonable period of time

for completion of repairs or replacement.

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Technical Specifications

Wavelength range 325nm to 1100nm

Monochromator Flat grating

Wavelength calibration Automatic upon switch on

Spectral bandwidth <7nm

Wavelength accuracy ± 2nm

Wavelength reproducibility ± 1nm

Light sources Pulsed Tungsten halogen

Detector CMOS array

Photometric range - 0.300 to 2.500A, 0.3 to 199%T

Photometric linearity ± 2.0 % or ± 0.010A to 1.000A at 546nm,

whichever is the greater

Photometric reproducibility < 0.002 A at 0A and 500nm

Stray Light < 1%T 340nm

Stability ± 0.005A/h at 0A and 546nm after warm-up

Noise ± 0.002A near 0A and ± 0.020A near 2A at

600nm

Digital output USB memory stick data storage

USB PC connection with PVC software

Data Export PC via USB cable

USB memory stick

Method Storage 90 with PIN number protection

Graphical Display Yes, zoom and track function

Sample ID Yes

Languages English, German, French, Spanish, Italian,

Japanese, Chinese

Dimensions 120 x 230 x 320 mm

Weight 1.55 kg

Power input 18 Vdc at max 18VA from a supplied 100 to

240V~, 50/60 Hz Mains Power Adapter

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Operation

Introduction

Your spectrophotometer is a simple-to-use visible instrument. It has no moving parts, which is the basis of the rapid

scanning operating system. It has been designed to meet the routine spectroscopy needs of customers requiring a

compact instrument that is easy to use. The product is reliable and requires low maintenance.

The user interface is built around folders which are displayed on the home page when the instrument is turned on. After it

is turned on and calibrated, the default home page offers the following choices:

Applications General spectroscopic methods

Favourites A folder to store your more frequently used configured methods

Methods Contains nine folders that can store less frequently used configured methods (nine

methods per folder)

Utilities Instrument set up (date, time, language, printer options, preferences and display settings)

Life Science Standard Life Science methods such as nucleic acid assays, protein assays and cell

counting

The instrument is supplied with a program, PVC (Print via Computer) on the accompanying CD. When used with a USB

cable to connect to a PC onto which the software has been installed, it enables the user to “print through” the PC directly

to the printer that is connected to it. The data may also be stored as an Excel spreadsheet, as an EMF graphics file, a

comma delimited (csv) data file, a tab delimited (txt) data file or in native PVC format for later access.

The instrument has a USB connector located in the front of the unit which allows data to be stored on a USB memory

stick. This data can be stored in a PVC format which can be opened using PVC software or csv data file that can be

imported into Excel.

Sample Handling Tips

The light beam is directed from RIGHT to LEFT through the cell chamber; therefore please ensure the cell is inserted

with the correct alignment.

The cell holder supplied with the instrument accepts standard 10 mm path length quartz, glass or plastic cells.

The optical height is 15 mm

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Keypad and Display

The back-lit liquid crystal display is very easy to navigate around using the alphanumeric entry and navigation arrow keys

on the hard wearing, spill proof membrane keypad.

Key Action

On/Standby

Key Turns the instrument on or back to standby.

Arrow Keys Use the four arrow keys to navigate around the display and select the required

setting for the active (highlighted) option.

View Options Used to select items in a menu or to enter parameters and text descriptions.

Repeated key presses can be used to cycle through lower case, upper case,

and numbers. Wait 1 second before entering the next character. Use the “C”

clear key to backspace and the 1 key to enter a space

Alphanumeric

Keys

Use to enter parameters and to write text descriptions where appropriate or

required. Use repeated key presses to cycle through lower case, upper case,

and numbers. Wait 1 second before entering the next character. Use the “Esc”

key to backspace and to enter a space

Escape/

Cancel Escape from a selection and return to the previous folder. Stop making

measurements.

OK Confirm parameters/selection.

Set

Reference

Set reference to 0.000A or 100%T on a reference solution at the current

wavelength in the measurement mode selected. When in scan mode, do a

reference scan.

Take a

Measurement Take a measurement.

Alphanumeric Keys

On/Standby

Key

Arrow Keys

View Options

Escape/Cancel

Set Reference

Take a Measurement

Red light will appear

when USB is inserted

OK Key

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Software Style

The user interface is built around having folders of files which are displayed on the home page when the instrument is

switched on. Different folders are numbered and opened by using the associated number key on the keypad.

Folder Keypad Number Description

1Single wavelength, Concentration, Wavelength scan,

Kinetics, Standard Curve, Multiple wavelengths and

Ratio.

2Saved User selected and configured methods

(maximum of 9 methods).

3Sub folder selection for User selected and configured

methods (9 sub folders each, containing a maximum of

9 methods).

4Instrument set up (date, time, language, data output,

preferences and display settings).

5 Proteins and Cell counting.

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TheApplications Folder

Options

Within each application the user has the possibility to select various options that define the way results are treated. If not

using a stored method, it is advisable to check that these Options have been appropriately set for your experiment when

coming to the instrument. Note that setting the “History” parameter to on (see Preferences later) will cause the instrument

Function Keypad Number Description

1Absorbance or %T (transmission) at a single user defined

wavelength.

2Concentration measurement at a single wavelength based on

a simple Factor entered or calculated from a single standard.

3Wavelength scan between two user-defined wavelengths.

Range 330-950 nm, with user configurable peak finding

function.

4Absorbance versus time measurements either rate or end

value based.

5Generation of calibration curve by measuring standards at a

single wavelength.

6Absorbance or %T (transmission) at up to 5 user defined

wavelengths.

7Ratio of absorbance values at two user specified

wavelengths.

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