Fluidigm Callisto Manual de usuario
PN 100-7598 D1
Callisto System
USER GUIDE
Callisto System: User Guide
2
For technical support visit fluidigm.com/support.
Europe +33 1 60 92 42 40 | [email protected]m
Japan +81 3 3662 2150 | techsupportjapan@fluidigm.com
For Research Use Only. Not for use in diagnostic procedures.
Information in this publication is subject to change without notice. It is
Fluidigm policy to improve products as new techniques and components
become available. Therefore, Fluidigm reserves the right to change
specifications at any time. Every effort has been made to avoid errors in
the text, diagrams, illustrations, figures, and screen captures. However,
Fluidigm assumes no responsibility for any errors or omissions. In no
event shall Fluidigm be liable for any damages in connection with or
arising from the use of this publication.
Patent and Limited License Information
Fluidigm products are covered by issued and pending patents in the
United States and other countries. Patent and limited license information
is available at fluidigm.com/legalnotices.
Limited Use License to Perform Preamplification with Fluidigm IFCs
A license to use Thermo Fisher Scientific’s patented preamplification
method workflows involving a Fluidigm integrated fluidic circuit (IFC) can
be obtained (i) with purchase of a Fluidigm IFC from Fluidigm Corporation
or (ii) by a separate license from Thermo Fisher Scientific. For licensing
information, contact outlicensing@lifetech.com.
Limited Digital PCR License
A license to use Thermo Fisher Scientific’s patented digital PCR method
in all fields other than in the Sequencing Field, the Mass Spectrometry
Field, and the Prenatal Field in workflows involving a Fluidigm IFC can be
obtained (i) with purchase of a Fluidigm IFC from Fluidigm Corporation or
(ii) by a separate license from Thermo Fisher Scientific. For licensing
information, contact outlicensing@lifetech.com.
Trademarks
Fluidigm, the Fluidigm logo, C1 and Callisto are trademarks or
registered trademarks of Fluidigm Corporation in the United States
and/or other countries. All other trademarks are the sole property of
their respective owners.
For EU's WEEE directive information, go to fluidigm.com/compliance.
© 2017 Fluidigm Corporation. All rights reserved. 10/2017
Callisto System: User Guide 3
Contents
About This Guide 5
Purpose 5
Safety Alert Conventions 5
Safety Alerts for Chemicals 5
Safety Alerts for Instruments 6
Safety Data Sheets 6
Chapter 1: Introducing the Callisto
System 7
Fluidigm Technical Support 7
Consumables Ordering 7
Components of the Callisto System 8
System Software 11
Components Included in Shipping Box 12
User Documentation 12
System Functions 12
Pre-Mixed Gas Use 14
Air Options 14
Chapter 2: Getting Started with
Callisto 15
Unpacking and Installing the Callisto System 15
Start the Callisto System 15
How to Use the Callisto Touchscreen 18
Run an Experiment 21
Load the Callisto Adherent Cell Culture IFC-EC
Interface Plate Assembly in the Instrument 21
Select and Run an Experiment 23
Perform Step Operations 28
All Steps 28
Feeding and Dosing Steps 28
Modify an Experiment 29
Skip a Step 29
Modify the Duration of a Feeding or Dosing
Step on Callisto 31
Modify an Experiment Remotely 32
Modify an Experiment with a USB Drive 32
Import and Export Experiment Plans and
Execution Files 34
View System Information 35
Export a Log 35
View Experiment Runs 36
Set a Personal Identification Number (PIN) to
Access the Instrument Remotely 36
Sort and Show Experiments 37
Shut Down the System and the Instrument 37
Chapter 3: Customizing Callisto 40
Log In 40
Log Out 40
Manage Users 41
Change the Date and Time 44
Update the System 46
Enable Remote Access 47
(Optional) Enable Compressed Air 48
Appendix A: Troubleshooting 50
Observation and Possible Course of Action 50
Powering On and Off 50
Loading 51
Running 52
Environmental Control Warnings 53
Appendix B: Maintenance,
Decontamination, and Disposal 54
Cleaning and Maintenance 54
Clean the Thermal Chuck 54
Callisto System: User Guide
Contents
4
Cleaning the Cell Culture EC Interface Plate 55
Replacing Filters in the EC Interface Plate 57
Cleaning the Touchscreen 59
Preventive Maintenance 59
Disposal of IFCs 59
Fuse Replacement 59
Decontamination of the Callisto System 61
Biological Agents 61
Hazardous Chemicals 61
Radioactive Materials 61
Appendix C: Related Documentation 62
Appendix D: Safety 63
General Safety 63
Instrument Safety 63
Symbols on the Instrument 64
Electrical Safety 66
Chemical Safety 66
Callisto System: User Guide 5
About This Guide
IMPORTANT Before using the instrument, read and understand the safety
guidelines in this document. Failure to follow these guidelines may result in
undesirable effects, injury to personnel, and/or damage to the instrument or to
property. In addition, ensure that you have installed or upgraded to Callisto™
Experiment Planner v2.0 or later on your computer and Callisto system software v3.8
or later on the instrument. [See Update the System on page 46 and the Callisto
Experiment Planner User Guide (PN 100-8806).]
Purpose
This guide describes how to use the Callisto system, including the instrument, the
integrated fluidic circuits (IFCs), and the system software.
For instrument specifications, see the Callisto System Site Requirements User Guide
(PN 100-7601). For the cell culture protocol using the Callisto Adherent Cell Culture
IFC, see the Callisto Getting Started Guide (PN 100-7599).
Safety Alert Conventions
Fluidigm documentation uses specific conventions for presenting information that
may require your attention. Refer to the following safety alert conventions.
Safety Alerts for Chemicals
For hazards associated with chemicals, this document follows the United Nations
Globally Harmonized System of Classification and Labelling of Chemicals (GHS) and
uses indicators that include a pictogram and a signal word that indicates the severity
level:
AZ
Indicator Description
Pictogram (see example) consisting of a symbol on a white background within a red
diamond-shaped frame. Refer to the individual safety data sheet (SDS) for the applicable
pictograms and hazards pertaining to the chemicals being used.
DANGER Signal word that indicates more severe hazards.
WARNING Signal word that indicates less severe hazards.
Callisto System: User Guide
About This Guide
Safety Data Sheets
6
Safety Alerts for Instruments
For hazards associated with instruments, this document uses indicators that include
a pictogram and signal words that indicate the severity level:
AZ
Safety Data Sheets
Read and understand the SDSs before handling chemicals. To obtain SDSs for
chemicals ordered from Fluidigm, either alone or as part of this system, go to
fluidigm.com/sds and search for the SDS using either the product name or the part
number.
Some chemicals referred to in this user guide may not have been provided with your
system. Obtain the SDSs for chemicals provided by other manufacturers from those
manufacturers.
Indicator Description
Pictogram (see example) consisting of a symbol on a white background within a black
triangle-shaped frame. Refer to the instrument user guide for the applicable
pictograms and hazards pertaining to instrument usage.
DANGER Signal word that indicates an imminent hazard that will result in severe injury or death
if not avoided.
WARNING Signal word that indicates a potentially hazardous situation that could result in serious
injury or death if not avoided.
CAUTION Signal word that indicates a potentially hazardous situation that could result in minor
or moderate personal injury if not avoided.
IMPORTANT Signal word that indicates information necessary for proper use of products or
successful outcome of experiments.
Callisto System: User Guide 7
Chapter 1: Introducing the Callisto
System
The Callisto™ system includes the Callisto instrument and the Callisto Experiment
Planner software. All consumables and reagents are available from Fluidigm. For
Callisto specifications, see the Callisto Specification Sheet (PN 101-1405).
Callisto controls pressure, thermal, and gas conditions in the Callisto Adherent Cell
IFC (Culture Integrated Fluidic Circuit). Specifically, these conditions are regulated by
an environmental control located above the IFC in the instrument.
Callisto is versatile. The IFC has two cell inputs, 16 reagent inputs, three common
medium inputs, and 32 addressable culture chambers to maintain 20–3,000
adherent cells per cell culture chamber. The system can support long-term cell
culture and viability for greater than three weeks. During and after the experiment
you can image live or fixed cells. You can harvest lysates for genomic analysis or
recover live cells for single-cell genomics on the C1™ system.
NOTE For Research Use Only. Not for use in diagnostic procedures.
Fluidigm Technical Support
Fluidigm technical support welcomes your questions or comments about the
configuration and use of the Callisto system. For phone or email contact information,
see page 2.
Consumables Ordering
To reorder IFCs and reagents, contact your regional Fluidigm sales representative or
distributor. Go to fluidigm.com/contact.html.
Callisto System: User Guide
Chapter 1: Introducing the Callisto System
Components of the Callisto System
8
Components of the Callisto System
Figure 1. Front panel of the Callisto instrument
Figure 2. Back panel of the Callisto instrument. There are also two USB 2.0 ports on the side
of the instrument.
Touchscreen
interface
Retracted tray
Power and
standby indicator
USB 2.0 ports
Ethernet
port
Digital visual
interface
(DVI) port
On/off switch
Power socket
Compressed air input
Exhaust fan
Pre-mixed gas input
Callisto System: User Guide
Chapter 1: Introducing the Callisto System
Components of the Callisto System
9
Figure 3. Tray and thermal chuck of the Callisto instrument. The white notch is used to orient
the notched corner of the IFC in the tray. The thermal chuck controls the temperature of the
cells by heating and cooling the IFC.
White notch
Thermal chuck
Callisto System: User Guide
Chapter 1: Introducing the Callisto System
Components of the Callisto System
10
Figure 4. Exploded view of the Callisto Adherent Cell Culture IFC-environmental control (EC)
interface plate assembly on the Callisto shuttle. The EC interface plate lies on top of the IFC.
Barcode numbers on the IFC label face out. The environmental chamber is the space between
the EC interface plate and the center of the IFC.
EC interface plate
Callisto Adherent Cell
Culture IFC
Barcode
Environmental chamber
Sensor
White notch
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