Etaluma LS820 Manual de usuario
LS820 Microscope
Operator’s Manual
With
Etaluma’s LumaviewPro™Software
Pre-Release Version 0.8
Etaluma, Inc.
Carlsbad, California
www.etaluma.com
This document is available for download at http://etaluma.com/products/downloads.
Lumascope™and LumaviewPro™are trademarks of Etaluma, Inc.
2009-2023 All rights reserved
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LS820 Microscope Manual 2023
Setting up the microscope
Items Included With Each LS820 Microscope
AuraPhase Transmitted Illumination
LED Fixture with cable
Optional ambient light guard
Fixture mounting knob
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LS820 Microscope Manual 2023
Accessories
A. Dichroic cleaning swabs
B. Black microplate lid for blocking room light during fluorescence imaging
C. Foam plug for incubator port
D. Lens paper
E. USB memory stick with LumaviewPro application and this manual
F. Mousepad
Optional Labware Holders
■
35 mm Petri dishes, fits inside Holder for 60 mm Petri dishes
■
60 mm Petri dishes & Terasaki plates
■
Microscope slides & 50 mm Petri dishes
■
4 microscope slides in parallel plastic
■
4 microscope slides in parallel metal with magnetic clamps
■
Single slide and dish with adjustable clamping
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LS820 Microscope Manual 2023
Connecting the Cables
Connect the USB type C end to the LS820 at the rear left corner of the microscope. Wait to connect the type A USB
cable from the Microscope to the computer until LumaviewPro is installed.
Plug the external power supply/cord into the rear port and the plug into an AC outlet. An extension cord is
provided in cases where the AC outlet is distant.
Aura Phase Transmitted Illumination Accessory
Remove the Aura Phase from its shipping box. Attach it by placing the holes on the bottom of the bracket over the pegs on the
upper surface. Tighten the large thumb screw beneath the deck cup.
Connect the free end of the Aura Phase Accessory power cable to the round socket nearby. This allows the Phase Accessory to be
controlled by LumaviewPro.
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LS820 Microscope Manual 2023
LumaviewPro
The LS820 is controlled by the LumaviewPro software program. The latest LumaviewPro version is downloadable from
Etaluma’s website and should be installed prior to connecting your computer for the first time.
LumaviewPro can be run on Windows10/11, Mac, and Linux. Desktop computers and laptops can be used, but the
best visualization correlates with monitor resolution equal to the sensor resolution (up to 2100x 2100 pixels). Note:
The monitor does not affect image resolution unless the monitor is low quality and affects your ability to judge focus.
Downloading and Installing LumaviewPro
To download Lumaview, go to http://etaluma.com/products/downloads (under the Resources tab). Click on LumaviewPro
- ZIP link foryour operatingsystem to start the download and save the folder when prompted. Go to your downloadslocation
and click to open the LumaviewPro.zip file.
Alternatively, the same LumaviewPro.zip file can be copied from the flash drive that comes with the microscope. It
may be helpful to verify that this file is the latest version as posted on the Etaluma website.
After installation, a LumaviewPro shortcut (orange logo icon) convenient for launching the software will be present on
the desktop.
Connecting the LS820 Microscope
Insert the standard USB-A end of the supplied USB cable into a USB port on your computer and the other USB-C end into
the port labelled “Computer”.It is also important to connect the Microscope directly to the computer USB port and not
use a USB hub.
Plug the barrel connector from the AC power supply into the port labelled “Power Input 24VDC”. A 2 meter extension is
available if required.
Pass the communication and DC power cable through the incubator port, which is often located on the upper back wall.
A plug or filtered stopper may be present. The power supply transformer should not be placed in the incubator, only the
24 VDC cable and its extension. The transformer should not be left hanging by the DC cord and should be supported or
attached to the back of the incubator. Every microscope comes with a foam plug that can be used to seal around the
cables going through the port or the existing plug can be adapted. Make sure there is enough slack in the cables inside
the incubator to accommodate any sliding out of the shelf. Place the computer on a flat and stable surface near the
incubator and within the reach of the 3 meter USB cable provided.
WARNING: Do not extend the USB cable or use another USB cable other than that
supplied as communication issues will arise. To extend the user interface to
microscope distance use a long HDMI for the monitor and wireless mouse and
keyboard. See Appendix D.
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LS820 Microscope Manual 2023
Getting Started with LumaviewPro
Launch the LumaviewPro software from the desktop icon (or other chosen location) and allow the microscope
to be discovered and initialize.
Launching LumaviewPro opens the application as seen below:
The side menus are accessed by clicking the blue arrows on the right and left edge of the application.
Motion Control Panel Image Control Panel
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LS820 Microscope Manual 2023
Microscope Settings
Click on the right arrow to expand the Image control menus.
Microscope Settings configures your hardware with model and objective settings.
Ensure that the correct model microscope being used is checked.
Click the Objective field to select the desired objective from the dropdown.
Channel Control
Each channel is controlled with Illumination, Gain, and Exposure. Gain has a checkbox that enables AutoGain. Checking
AutoGain will cause it to be used for that channel in any Protocol saved with that channel. The channel LED can be turned on and
off here. The Colorize option provides pseudo coloring for the Fluorescence channels. Checking the Acquire option will include
this channel in any automated process including Protocols, Composites, and Z-stacks. Individual channels can have a Saved Focus
position which will be used in the Protocol for that channel. Folder and filename root are configured for each channel for
Protocol data. A live histogram of pixel intensities is displayed with linear or logarithmic Y axis.
There are three transmitted channels- Brightfield, Phase Contrast, and Extended Phase. Brightfield illumination is from a central
LED with an appropriate lens for uniform illumination. Phase Contrast is a small ring of LEDs that match the Ph1 objectives we
supply from Evident. Extended Phase is a larger ring of LEDs that is currently under development to provide Darkfield.
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LS820 Microscope Manual 2023
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LS820 Microscope Manual 2023
Focus and Z-Control
The Focus control allows manual adjustment of the focus position using coarse and fine arrows according to the objective
installed. You may drag the objective icon up or down for very large movements. Clicking within the objective icon will adjust
focus based on the position relative to the center of mass of the icon. Home brings the optics to the fully retracted position. “Set
Z” will memorize the current position and allow you to easily return. “Set All Z”will record the position into each channel’s
individual focus setting. Home retracts the objective to the lowest level. Autofocus uses image contrast to find the best focus
according to the objective installed.
Autofocus scans through a range defined by the objective magnification with a coarse and then finer z axis step size monitoring
contrast and finding its maximum. Autofocus failures can be due to the incorrect magnification objective in the Microscope
Settings as well as a variety of image quality issues such as high background, thick samples, and very few edges from which to
calculate the contrast.
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LS820 Microscope Manual 2023
Getting an Image
Place the sample onto the microscope and ensure it is seated flat. Enable an illumination channel and adjust the focus to find the
image. Adjustments to the Illumination, Gain and Exposure may be required to center the pixel brightness histogram for that
channel. Click Save Focus for that channel. It may be convenient to Set Z under the Focus tab to return to this location.
The Toolbar
The Toolbar provides common actions. From left to right, define the destination folder for non-Protocol files, pause with a freeze
frame, acquire an image of the live image, acquire a composite image according to the individual channel settings Acquire
checkbox, record the live image video stream, fit the live image to the application image display window, and present the image
a one camera pixel to one screen pixel.
Protocol
To configure an automated microscopy run, including time-lapse, the Protocol feature is utilized.
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