Enzo Absorbance 96 Manual de usuario

Rev 052021

Product Manual

Absorbance 96 plate reader

Catalog #: ENZ-INS-A96

Manufactured by Byonoy GmbH

Device Version 2.0 (2021-01-18)

Software Version 1.2 (2021-01-18)

For the latest product information, including support documentation, visit us

online:

www.enzolifesciences.com

Technical Support (US): 800-942-0430

Technical Support (EU): +41 61 926 8989

Customer Service (US): [email protected]

Customer Service (EU): [email protected]

FOR RESEARCH USE ONLY.

NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Manufacturer:

Byonoy GmbH

Bernstorffstr. 118

22767 Hamburg, Germany

00 49 40 5379 866 00

service@byonoy.com

www.byonoy.com

Instructions for Use

Absorbance 96

Valid for REF Number ABS MH A 01/ABS MH D 01

Instructions for Use

Absorbance 96

Version 2.0 (18/01/2021)

Dear customer,

We are delighted that you have chosen the Absorbance 96. To take full advantage of the

instrument's performance and to enjoy your instrument for many years, please read these

instructions for use carefully before installation and commissioning. Operate the instrument

in accordance with these instructions. The operating-safety and function of the instrument

can only be guaranteed if both the general safety regulations and accident prevention reg-

ulations of the legislator as well as the safety instructions in this manual are observed. We

accept no liability for any damage resulting from improper use or incorrect operation.

Ensure that the manual is always accessible and is read and understood by all

persons operating the instrument.

This user manual may only be used according to its intended purpose. It may not be repro-

duced, changed, or translated in another language without the prior written consent of

Byonoy GmbH.

This document is subject to technical changes and updates.

III

TABLE OF CONTENTS

General information...................................................................................................................... 1

Overview of the Absorbance 96.......................................................................................... 3

2.1 Scope of application ................................................................................................................................. 3

2.2 Measurement method ............................................................................................................................. 3

2.3 Absorbance 96 System........................................................................................................................... 3

2.4 Consumables .................................................................................................................................................. 5

Preparing the product for use..............................................................................................6

3.1 Unpacking, storage, and transport ................................................................................................ 6

3.2 Supplied materials...................................................................................................................................... 6

3.3 Working environment .............................................................................................................................. 6

Operation ........................................................................................................................................... 8

4.1 App installation procedure and updates................................................................................... 8

4.2 Computer connection.............................................................................................................................. 8

4.3 Assay readout................................................................................................................................................ 9

Signals and troubleshooting................................................................................................ 10

5.1 Signal lights.................................................................................................................................................... 10

5.2 Troubleshooting ........................................................................................................................................ 10

Maintenance and cleaning .................................................................................................... 12

6.1 Maintenance ................................................................................................................................................. 12

6.2 Cleaning............................................................................................................................................................ 13

6.3 Technical support..................................................................................................................................... 15

6.4 Repairs .............................................................................................................................................................. 15

Technical specifications ......................................................................................................... 16

Guarantee ........................................................................................................................................ 18

EC-Conformity Declaration................................................................................................... 19

General information

Important: Follow the instructions for use

Any commissioning or handling of the instrument requires precise knowledge and notice of

this manual. The device is intended for the described use only.

In this manual, particularly important remarks are labeled as followed:

Warning:

This is a warning that indicates an immediately or potentially dangerous situa-

tion, which, if not avoided, will result in death or serious injury.

Caution:

Caution indicates a potentially hazardous situation, which, if not avoided, could

result in minor or moderate injury.

Information:

This is a piece of information indicating certain properties that must be ob-

served.

Liability for function and damage

Liability for the function of the device shall, in any case, pass to the owner or operator if the

device is improperly maintained, repaired, or modified by persons who do not belong to the

authorized service personnel, or if it is handled in a way that does not comply with its in-

tended use. The service and operation of the product must be in accordance with this man-

ual. Byonoy shall not be liable for damages resulting from non-observance of the information

above. Warranty and liability conditions of the terms of sale and delivery of Byonoy are not

extended by the information above.

Warning

An incorrect working environment can lead to a reduction in service life, dam-

age to the device, or measurement errors. Please note the explanations and

warnings in chapter 3.3.

To avoid measurement errors, it is essential to ensure that the instrument is

connected properly and that the experiments are carried out correctly. Please

note the explanations and warnings in chapter 3.

Incorrect cleaning of the instrument can reduce its service life and can cause

damage to the instrument. Please note the explanations and warnings in chap-

ter 6.2.

Information

Follow all safety instructions on the device and in the attached documents.

General information

Follow all general precautions that apply to electrical instruments.

Disposal of used electrical and electronic equipment

The symbol on the product or its packaging indicates that this product is not to be treated

as normal household waste. It must be disposed of at a collection point for the recycling of

electrical and electronic equipment. By contributing to the correct disposal of this product,

you protect the environment and the health of your fellow human beings. Recycling helps to

reduce the consumption of raw materials. For further information on how to recycle this

product, please contact your local authority or municipal waste disposal centres.

Working with biological and harmful material

The Absorbance 96 is not to be used for the measurement of biohazardous substances.

Always observe the manufacturer's hazard information pertaining to the substances to be

measured.

The instrument does not produce any toxic or harmful gases or substances. During the

measurement, make sure that there are no toxic or harmful substances in the microtiter plate.

Overview of the Absorbance 96

2.1

Scope of application

The Absorbance 96 is an optical laboratory instrument for measuring the absorbance (opti-

cal density) of biological or non-biological samples in ANSI/SBS-standard 96-well microtiter

plates in accordance with the specifications described in the user manual. The Absorbance

96 is intended for research and other non-in-vitro-diagnostic analyses only. It is to be oper-

ated by trained laboratory personnel and is intended for professional use.

2.2

Measurement method

The Absorbance 96 is designed to carry out sensitive absorbance measurements. It

measures the optical density (OD) of samples at defined wavelengths.

Absorption

Absorption refers to the amount of light absorbed by a medium. Absorption reduces trans-

mission. Transmission is the ratio of incident to transmitted light. Accordingly, the degree of

transmission is calculated as follows

T = (I/I0)

where I is transmitted light and I0is incident light.

Optical Density

Optical density is a measure of the attenuation of light radiation after it has passed through

a medium. Optical density is the logarithmic quantity that describes the reciprocal of the

transmittance T:

OD = log(I0/I)

where I is the transmitted light and I0is incident light.

Optical density is the absorbance of the sample plus other attenuating effects such as scat-

tered light due to turbidity. It is therefore necessary to avoid scattered light in order to meas-

ure absorbance correctly.

If no other attenuating effects are present, absorbance = optical density.

2.3

Absorbance 96 System

Absorbance 96 Reader

The Absorbance 96 reader is an absorbance-based measuring device, i. e. a measuring in-

strument, with which the absorbance values of a 96-well microtiter plate can be read, rec-

orded and provided for further processing. The Absorbance 96 is a solid-state microplate

reader and has 96 detection units, allowing measurement without a scanning mechanism.

Overview of the Absorbance 96

In the upper part of the measuring instrument, there are up to 4 LED light sources with dif-

ferent wavelengths and up to 4 interference filters. The four signal lights on the top of the

reader show which measuring channel is being used for the current measurement.

The microtiter plate is inserted manually into the slot of the device. There are two raised

locking-edges at the bottom of the slot, as well as an arrow for visual inspection. The arrow

becomes wholly visible only after complete insertion of the microtiter plate into the slot.

Figure 1.

Visual representation of the product from the front, top. 1. Signal lights. 2. Slot for

microtiter plate. 3. Raised locking-edges. 4. Arrow.

On the back of the Absorbance 96 there is a Micro-USB port for connecting the instrument

to a computer via the included Micro-USB cable. Furthermore, there is an eject button for

ejecting the microtiter plate out of the slot.

Figure 2.

Visual representation of the product from behind, top. 5. Eject button. 6. Micro-

USB port.

Power consumption

The Absorbance 96 is an analytical instrument with very low power consumption. It is oper-

ated by a Micro-USB cable and the total power consumption is generally less than 2.5 watts.

Absorbance 96 App

Overview of the Absorbance 96

The reader is controlled via the Absorbance 96 App. The operation of the app is described

in-detail in a separate user manual.

2.4

Consumables

With the Absorbance 96, ANSI/SBS Standard 96-well microtiter plates can be used. Only

certain microplate types can be used with the Absorbance 96 (see chapter 7).

When using microtiter plates, always check the specifications of the microtiter plate manu-

facturer. Not all microtiter plates of a particular manufacturer are the same in design, mate-

rials, or configuration. The temperature stability within the microtiter plate can depend on

the type of microtiter plate used.

Preparing the product for use

3.1

Unpacking, storage, and transport

Unpacking

Remove the packaging material and carefully place the device on a firm and level surface.

Check the device for external damage and check the materials supplied (see chapter 3.2).

The device is packed in a specially designed cardboard box. Keep the packaging material. If

the device must be returned for repair, the original packaging material must be used.

Storage and protection during the intervals of normal use

Protect the device from moisture and dust during prolonged storage.

Do not store the device next to heat sources and protect it from direct sunlight.

The temperature for storage should be within the recommended temperature range (see

chapter 7).

Transportation

Before transportation, unplug the instrument and ensure there is no microtiter plate inside

the device. Depending on the transport distance, use the original packaging material. Make

sure that the new location meets the requirements described in chapter 3.3.

To avoid spillage and contamination, make sure that there is no microtiter plate

inside the instrument before transport.

Always transport the product well-packaged to avoid damage in transit.

3.2

Supplied materials

When unpacking the device, please check that the following components are present:

• Absorbance 96

• Micro-USB cable

• Hex key

• Microfiber cloth

• USB Stick

3.3

Working environment

Due to its small size, the Absorbance 96 can be operated very well in various working envi-

ronments.

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