Edmund Optics TECHSPEC Manual de usuario

STEP-BY-STEP INSTRUCTIONS FOR

BUILDING A FLUORESCENCE

MICROSCOPE

TECHSPEC®

Optical Cage System

INTRODUCTION 2

What is a Digital Fluorescence Microscope?

•Unlike traditional microscopes, which utilize an eyepiece for performing visual inspection,

digital video microscopes utilize cameras to record and capture images, providing higher

resolution and higher precision than what is typically seen with the human eye. Since

infinity-corrected digital video microscopes can be quite complex, using the modular

TECHSPEC Optical Cage System is ideal for simplifying the assembly. In this case

another aspect has been added to the design to incorporate a fluorescence aspect to the

digital microscope. Fluorescence microscopy is ideal for measuring and analyzing the

absorption and excitation of various wavelengths of light. An in-line fluorescence

microscopy setup utilizes a dichroic filter to redirect light from an illuminator into the

perpendicular optical path.

Why TECHSPEC Optical Cage System?

•Building a customized digital fluorescence microscope with TECHSPEC Optical Cage

components allows the user to build a microscope quickly and easily with limitless

customization options. Objectives can be changed to modify system magnification, and

interchangeable filter holders make swapping filters easy. Custom illumination can be

added for bright field, dark field, or in-line illumination options.Additionally, a

spectrometer could be added for precise spectral measurements.

OVERVIEW 3

Complete Parts List

Filter Selection

Sub Assemblies:

1. Dichroic

Assembly

2. Objective Arm

3. Source Arm

4. Imaging Arm

Mounting & Alignment

COMPLETE PARTS LIST 4

STOCK # Description QUANTITY

85713 25.4mm Dia. Adapter for 85710 1

85710 Square Tip/Tile Optic Mount 1

Depends on Application

25mm Dia. Dichroic Filter 1

85624 Cage Sphere with (5) 30mm Port and (1) 43mm Port 1

85632 25mm Standard Cage Plate with M6 for Post Mounting 3

38944 0.316” ID, Fiber Optic Adapter SX-6 2

56371/56372

115V AC 50/60HZ, MH-100 Illuminator/220V AC 50/60HZ, MH-100

Illuminator 1

42346 1/4" x 24”, Flexible Fiber Optic Light Guide 1

85588 30mm Cage 25/25.4mm Diameter Thick Lens Mount 1

85737 30mm Square Interface Plate 5

85719 30mm Cage Plate C-Mount Adapter 3

55743 Mitutoyo to C-Mount 10mm Adapter 1

46143 5X Mitutoyo Plan Apo Infinity-Corrected Long WD Objective 1

85488 Cage Support Rod 6mm Diameter x 100mm Length, M3 4

85486 Cage Support Rod 6mm Diameter x 50mm Length, M3 2

85490 Cage Support Rod 6mm Diameter X 150mm 4

85630 30mm Standard Cage Plate with M6(1 for Post Mounting) 3

86018 Filter Holder Plate 2

85649 Interchangeable Filter Holder 2

Depends on Application

Fluorescence Bandpass Filters (see details) 2

54774 MT-1 Accessory Tube Lens 1

58329 Mitutoyo MT-1/MT-2 C-Mount Adapter 1

85492 Cage Support Rod 6mm Diameter x 300mm Length, M3 4

54633 C-Mount Extension Tube (100mm Length) 1

54632 C-Mount Extension Tube (50mm Length) 1

03625 C-Mount Fine Thread Extension Tube (30mm-50mm Length) 1

65553 25mm Dia. 25mm Efl. Achromatic Lens 1

66014 25mm Dia. 20mm Efl. Aspheric Lens 1

59367 EO-3112C ½” CMOS Color USB Camera 1

59382 5m USB2.0 (mini-B to standard-A) Cable 1

85618 Micrometer Mount Kit 1

58661 Micrometer Head Anvil 13mm 1

OPTICAL FILTERS 5

A basic fluorescence microscope requires 3

filters. Each application will use a different

combination depending on the system

requirements:

•Excitation Filter: placed within the

illumination path of a fluorescence

microscope. It filters out all wavelengths of the

light source except for the excitation range of

the fluorophore or specimen under inspection.

•Dichroic Filter: placed between the excitation

filter and emission filter at a 45° angle. It

reflects the excitation signal towards the

fluorophore under inspection and transmits the

emission signal towards the detector.

•Emission Filter: placed within the imaging

path of a fluorescence microscope. It filters out

the entire excitation range of the fluorophore

under inspection and transmits the emission

range of the fluorophore.

1. DICHROIC ASSEMBLY

DICHROIC ASSEMBLY 7

STOCK # Description QUANTITY

85713 25.4mm Dia. Adapter for 85710 1

85710 Square Tip/Tile Optic Mount 1

N/A 25mm Dia. Dichroic of choice (see details under filter) 1

85624 Cage Sphere with (5) 30mm Port and (1) 43mm Port 1

85632 25mm Standard Cage Plate with M6 for Post Mounting 1

85737 30mm Square Interface Plate 1

86018 Filter Holder Plate 2

Parts needed for this step:

DICHROIC ASSEMBLY 8

1. Carefully insert the 25mm Dia. Dichroic

Filter into the 25.4mm Dia. Adapter (#85-

713). Lock into place with the M3 set

screws. Insert the adapter into the Square

Tip/Tilt Optic Mount (#85-710) and secure.

2. Attach the 25mm Standard Cage Plate

(#85-632) to the Cage Sphere (#85-624)

opposite the 43mm port with M3 SHCS.

Slide the base of the tip/tilt assembly from

step 1 into the sphere and secure within the

plate. (Alignment will be done later).

3. Attach two Filter Holder Plates (#86-018)

with M3 SHCS to two adjacent sides of the

sphere. Keep the the openings of the plates

facing the 43mm port. Attach a Square

Mounting Plate (#85-737) to a side opposite

of a filter holder plate.

2. OBJECTIVE ARM

OBJECTIVE ARM 10

STOCK # Description QUANTITY

85737 30mm Square Interface Plate 2

55743 Mitutoyo to C-Mount 10mm Adapter 1

46143 5X Mitutoyo Plan Apo Infinity-Corrected Long WD Objective 1

85719 30mm Cage Plate C-mount Adapter 1

85490 Cage Support Rod 6mm Diameter X 150mm 4

85488 Cage Support Rod 6mm Diameter x 100mm Length 2

85-618 Micrometer Mount Kit 1

58-661 Micrometer Head Anvil 13mm 1

Parts needed for this step:

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