Edmund Bühler KS 15 control Manual de usuario

Art.-Nr. 0091010

KS 15 control TiMix 5 control

SM 30 control VKS 75 control

Bedienungsanleitung

Schüttler

Operating Instructions

Shaker

Mode d’emploi

Agitateur

VERSION 01 / 2021

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Thank you for having chosen an

original Bühler product.

Operating Instructions

Schüttler

KS 15 Control TiMix 5 control

SM 30 control VKS 75 control

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Content

1. General notes 6

1.1 General Notes Concerning Areas of Application and

Mode of Operation 7

1.2 Safety Instructions 8

2. Transport Instructions 8

3. Installation and Connection 9

4. Operating the models 10

4.1 Display layout 10

4.2 On/Off switch 11

4.3 Operating modes 11

4.3.1 Manual operating mode 11

4.3.2 Automatic operating mode 11

4.4 Switching on the device and selecting the operating mode 11

5. Manual operating mode 14

6. Automatic operating mode 18

6.1 Programming a shaking program 19

6.2 Starting a shaking program 21

6.3 Changing a shaking program 22

6.4 Deleting a shaking program 23

6.5 Delayed start 24

7. Alarm functions 25

7.1 Alarm tolerance rpm 25

7.2 Alarm temperature monitoring 25

8. Hot plate shaker function 26

8.1 Hot plate shaker in manual operation 26

8.2 Hot plate shaker in automatic operation 28

9. Parameters in the M1 menu 30

9.1 Overview of program steps in the M1 menu 30

9.2 Programming in the M1 menu 30

10. Parameters in the M2 menu 34

10.1 Overview of menu steps in the M2 menu 34

10.2 Programming in the M2 menu 38

11. Combination with Bühler thermohood TH 44

11.1 Working with the thermohood TH in manual operation 45

11.2 Working with the thermohood TH in automatic operation 46

12. Changing the motion 48

12.1 Universal Shaker SM 30 C control 48

12.2 Adjustment of the Counterweight (TiMix 5 control) 49

13. Exchange of Rack Systems of KS 15 control and TiMix 5 control 51

13.1 Compact Shaker KS 15 control 51

13.2 Microplate Shaker TiMix 5 control 51

14. Fastening of Multi-Storey Rack Systems 52

14.1 Microplate Shaker TiMix 5 control 52

14.2 Universal Shaker SM 30 A / B / C control 52

14.3 Multi Flask Shaker VKS 75 control 52

15. Maintenance and Servicing Instructions 53

15.1 Exchange of the Fuse 53

15.2 Motor Protection 53

16. Maximum Shaking Speed 54

16.1 Universal Shaker SM 30 54

16.2 Multi Flask shaker VKS 75 54

17. CE Declaration of Conformity 55

18. Warranty 56

19. Technical Data 57

20. Basic Equipment 58

21. Rack Systems and Loading Capacities 59

21.1 Rack Systems for KS 59

21.2 Additional Strips for Combiﬁx KS 60

21.3 Spring clamps and test tube racks 61

21.4 Loading Capacity of Rack System Combiﬁx KS 62

21.5 Loading Capacity of Universal Tray KS 62

21.6 Rack Systems for TiMix 5 63

21.7 Rack Systems for SM 64

21.8 Loading Capacity of Rack Systems Combiﬁx SM 67

21.9 Loading Capacity of Universal Tray SM 67

21.10 Rack Systems for VKS 68

21.11 Loading Capacity of Rack Systems Combiﬁx VKS 71

21.12 Loading Capacity of Universal Tray VKS 71

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1. General notes

You have chosen a Bühler high-quality product for supporting you in your

work. All Bühler shakers were developed for the use in laboratories in a

neutral environment. To ensure a long life and optimal operation of the device

we recommend to observe the following points.

Read the operating manual carefully before initial operation.

The user must acquaint himself with the safety instructions and

operating conditions in order to avoid damage / injuries to material

and personnel.

Liability and all claims under warranty end immediately in case of

damages which result from misuse and / or abuse.

The devices were carefully checked for perfect functioning and

condition before delivery.

Necessary servicing or repair work may only be done by

• personnel of the manufacturer (Edmund Bühler GmbH)

• their authorized agents

• personnel trained by Bühler

For shipping, the device must be adequately and safely packed.

If possible, use the original packing.

If the device is returned to Bühler for repair, it should be cleaned

and free from any harmful substances or residues.

These operating instructions are protected by copyright.

Modiﬁcations reserved.

1.1 General Notes Concerning Areas of Application and

Mode of Operation

The devices can be used in all laboratory ﬁelds in which it is necessary to mix and

shake homogeneously under constant and deﬁned conditions.

Areas of Application (Examples)

• Homogeneous mixing of different liquids as well as of solid and liquid

components (e.g. nutrient solutions)

• Shaking of kits for diagnostic tracing reactions

• Solvent extraction of different phases in separating funnels for chemical

investigations

• Evenly changing agitation of liquid phase (nutrient solutions) or gaseous phase

(cell culture in Petri dishes)

• Coloring and decoloring of gels

Thanks to their different motions and high loading capacities, and especially their

variable „Combiﬁx“ rack systems, Bühler shakers offer solutions both for general

as well as individual shaking tasks.

For keeping the samples at constant temperatures, the shaker models SM 30, KS 15

and TiMix 5 can be equipped with an additional incubator hood.

Temperature range: +5°C above ambient up to +50°C, optionally up to +60°C.

A cooling coil for connection of an external ﬂow-through cooler is available

as an option (TH 30).

Incubator Hood TH 15 (Order No. 6161 000)

Incubator Hood TH 30 (Order No. 6162 000)

In case of enquiries please contact the Export Sales Department

Phone: 0 74 71 / 98 64 - 0

Telefax: 0 74 71 / 98 64- 75

e-mail: info@edmund-buehler.de

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1.2 Safety Instructions

When shaking aggressive liquids / substances there is a risk of

injuries caused by splashing or spilling. Work with adequate

protective equipment only. In general, avoid splashing by choosing

a suitable shaking speed.

Due to the movements of the device there is danger of clothing

or body parts getting caught. During operation pay attention that

neither clothing nor jewellery get into contact with moving parts.

The maximum permissible shaking speed depends on the load.

See chapter 11 for max. shaking speeds.

2. Transport Instructions

Safe transport of the devices is only ensured if original packaging is used.

If they are bumped hard or put down roughly, damages can occur.

Do not lift the devices at the shaking plate (tray)!

Transport the devices by holding them at the housing only!

3. Installation and Connection

Place the shaker on a level, smooth and ﬁrm surface so that it stands ﬁrmly also at high

shaking frequencies.

For connection requirements and operating voltage see technical data as well as the

rating plate at the back of the device.

The system may only be connected to a mains with protection earth!

When installing the devices make sure that they are protected

against splash water.

The safety distance between the device and other instruments

or a wall must be chosen in such a way that the shaking plate is

freely movable and that the operating personnel cannot be injured

when the shaker is switched on or during operation.

If other tasks are performed by personnel in immediate vicinity of the

shaker, the shaker must be switched off for reasons of safety.

The shaker models VKS 75 are designed for ﬂoor operation only.

We strongly recommend to fasten the ﬁxing rings which are

included in the delivery to the ﬂoor.

They prevent the shaker from shifting during operation.

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4. Operating the models

4.1 Display layout

1Actual value for rpm or runtime

2Set value for rpm or runtime

3Date/time or remaining runtime

4Symbol for activating the powerfail function

5Direction of rotation (default R = right = clockwise)

6Button for activating the rpm display (=default display when the device

is switched on)

7Button for activating the time display

“Mode” button for changing the operating mode

Manual operation

Automatic

9Button for activating setting menu 1 and 2

10 Button for increasing the rpm or runtime

11 Button for reducing the rpm or runtime

12 “Play” button for starting the shaking movement or the runtime

13 Stop button for cancelling the shaking process

4.2 On/Off switch

The devices KS 15 control, TiMix 5 control, SM 30 control and VKS 75 control are

equipped with an On/Off switch.

This switch is positioned on the right-hand side of each device.

4.3 Operating modes

There are 2 different operating modes to choose from.

4.3.1 Manual operating mode

In this operating mode, the set values for rpm and runtime are manually set via the

touch display. The values can be changed at any time during the shaking

process. After the end of the shaking process, the

set values are stored until the shaker is switched off.

4.3.2 Automatic operating mode

In this operating mode, user-speciﬁc shaking programs can be stored. A shaking

program consists of different segments. A segment is deﬁned by rpm, runtime and

direction of rotation.

This operating mode is described in detail in Ch. 6

4.4 Switching on the device and selecting the operating mode

The device is switched on using the On/Off switch (on the right-hand side).

A switching-on routine and a self-test are performed.

During the switching-on routine, the following information is displayed for approx. 5

seconds.

• General shaker image

• Shaker type

• Software version

• Device number

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Example

For approx. 5 seconds, the display shows the

shaker type, the software version and the

device number.

After the switching-on routine, the device enters the operating mode

that was active before it was switched off.

Assumption in this case: The manual operating mode was active

before the device was switched off.

The device is in manual operating mode.

The rpm display shows the current rpm actual

value (=0).

Click the “Mode” button to open the mode

selection in the display. The manual operating

mode “Manual operation” is shown with the colour

inverted.

Click the “Automatic” button to select automatic

operation.

Click the “Mode” button again to go back to the

rpm display.

Click the button to open the menu selection in

the display. Menu 1 is freely accessible. Menu 2 is

password-protected.

14 15

Alternatively, you can click the rpm to open a keypad.

You can also specify the actual rpm using this keypad.

You must conﬁrm the speciﬁed rpm with “Enter”. The

keypad disappears again. However, the shaker does not

yet start.

Speciﬁed speeds below the minimum rpm and above the

maximum rpm are not accepted.

Speciﬁed speeds that fall between 2 rpm increments will

be rounded up or down (e.g. 108 is rounded to 110, 107

is rounded to 105).

The rpm can also be speciﬁed while the shaker is running.

After you specify the set rpm using the keypad and press

the “Enter” button, the set rpm is displayed in the upper

area and a “Play” button appears. After you click this

button, the shaker starts and the “Play” button disappears

again.

If you need to specify a different rpm, you can click the

rpm (here 0 rpm) to display the keypad again and enter a

different rpm set value.

You can also change the set rpm while the shaker is

running. However, rpm changes only take effect after you

click the “Play” button.

Press the “Stop” button to stop the shaker.

Click the “Clock” button to switch to the time display. The

“Clock” is shown with the colour inverted.

In this display, you can specify a runtime in hours, minutes

and seconds (h, min, s).

The shaker rpm is displayed in the upper right area.

Following the expiry of this time, the shaker comes to a

stop and an audible signal can be sounded as an option.

After it is switched on, the device is in the operating mode

that was active when it was last switched off (in this case,

manual operating mode).

The rpm display shows the current actual value for the

rpm (=0).

The direction of rotation is displayed at the bottom left.

The operator can specify this in the M1 menu (Ch. 10).

Click the “arrow up” button to specify the set rpm at the

top right. A “Play” button appears. Click this button to

start the shaker.

The set rpm is displayed in the upper area until it is

reached. This display ﬁeld then disappears.

By clicking the “arrow down” button, you can still reduce

the set rpm while the device is running up to rpm, for

example, if you mistakenly entered too high an rpm.

The rpm for the types KS 15, SM 30 and VKS 75 is gen-

erally entered in increments of 5 rpm. On the type TiMix 5,

the rpm is entered in increments of 10 rpm.

After the shaker stopped, the set rpm remains stored. You

could restart the shaker with the same rpm by clicking the

“Start” button.

To delete the set rpm, click the actual rpm (here = 0) for 2

seconds.

The set rpm is deleted. The “Start” button has disap-

peared.

5. Manual operating mode

Stop

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Example

The shaking process is almost complete (remaining run-

time 10s).

The shaking process is complete.

The “End” notiﬁcation appears in the display for a time that

can be adjusted. In addition, an optional audible signal

can sound for an interval that can be adjusted.

You can conﬁgure the “End” notiﬁcation in the M2 menu.

Click any point on the touch display to acknowledge the

“End” notiﬁcation.

The speciﬁed rpm and runtime settings will then reappear

in the display.

You can restart the shaking process with these values by

clicking the “Play” button.

After you click the hour display, you can set the hours

using the arrow buttons.

Follow the same procedure to enter the “min” and “s”

settings.

You can only change or delete the runtime when the

shaker is stopped. After adjusting the runtime, you must

restart it using the “Play” button.

In each case, the runtime starts again from the beginning.

After the runtime starts, the time in the display is counted

down. The set time remains visible at the top left.

The “Pause” button appears. Click this button to stop the

shaker. The remaining runtime is paused and the “Pause”

button is replaced by the “Play” button.

Click this button to restart the shaker. The remaining runt-

ime continues to count down.

You can use the “Pause” button if you only want to stop

the shaker brieﬂy without restarting the runtime from the

beginning.

If you click “h” for 2 seconds when the shaker is stopped,

the runtime “h” is reset to 00.

Follow the same procedure to reset “min” and “s”.

If you click one of the times (“h” or “min” or “s”) for approx.

3 seconds, this will initially reset the respective time unit to

0 and then the entire set time to 0.

Click the button to switch to the rpm display. The

remaining runtime is displayed in the upper left area.

You can press the button Stop to stop the shaking process

at any time. The set values for rpm and runtime remain

stored and are shown in the upper area. Press the “Play”

button to restart the shaking process with the same rpm

and the same runtime.

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6. Automatic operating mode

The “Automatic” operating mode enables the execution of deﬁned shaking programs.

These shaking programs consist of individual, deﬁnable segments. A single segment

consists of rpm, runtime and direction of rotation. This allows samples to be shaken in a

reproducible manner.

In total, you can deﬁne 10 different programs (P1–P10), each of which can consist of

10 segments (1–10).

The rpm “0” can also be deﬁned. This allows you to deﬁne e.g. an inactive

phase during the shaking process.

The “Automatic” operating mode is switched on as follows.

Assumption in this case: The manual operating mode was active before the device was

switched off.

Example

Click the “Mode” button to open the mode selection in the

display. The active operating mode (here “Manual opera-

tion”) is shown with the colour inverted.

Click the large “Automatic” button to select automatic

operation.

If you do not wish to switch to automatic mode, click on

the “Mode” button again to go back to the rpm display.

Clicking the large “Automatic” button brings you to the

program overview display. There, you can see the number

and name of the previously deﬁned shaking programs.

The buttons and are not active in automatic

mode.

The program numbers of previously deﬁned programs are

shown with the colour inverted.

A program does not have to have a name (see P2).

6.1 Programming a shaking program

A shaking program can consist of up to 10 segments.

To program a new shaking program, you must click

a free program number in the program overview

display (P4 here).

After you click the program row, a keyboard appears

in the touch display.

You can use this keyboard to enter a 20-character

program name.

However, a program does not have to have a name.

If you do not wish to use a program name, you can

simply click the OK button to continue. You can also

add a program name later on.

You can now see the program display for Program 4

without a name and an empty input ﬁeld for Segment

You are now in change mode. The button is

shown with the colour inverted. You can deﬁne

Segment 1.

Click the segment row to move it down slightly and

you can then deﬁne the values for segment no., rpm,

runtime and direction of rotation.

Use the “arrow up” or “arrow down” buttons to

specify the segment number. A numeric keypad is

used to specify the rpm.

To specify the segment runtime, click the time

display several times. After the ﬁrst click, use the

“arrow up” and “arrow down” buttons to set the

hours.

Click again to set the minutes and click again to

set the seconds.

The running direction is speciﬁed with the help of

the “arrow up” and “arrow down” buttons.

All entries must be conﬁrmed with the “Enter”

button.

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