PAGE 4 DIAGENODE BIORUPTOR® PICO USER MANUAL
Innovating Epigenetic Solutions
Critical steps for maintenance and
efficient shearing
General warnings
• DO NOT turn on the instrument without water.
• DO NOT tilt the Bioruptor® Pico. To change the water, use either the plastic pump or a beaker (be careful not to
scratch the bottom of the sonicaton bath).
Sonication bath levels
• The sonication bath must be filled with distilled water to the fill line. Fill line replacement stickers can be obtained by
contacting Diagenode.
• At least once per month.
Sonication bath temperature
• Optimal temperature for sonication is 4°C. Sample temperature should not exceed 8°C.
• The
Water Cooler
(New Cat. No. B0201002, 230V; B02010003, 115V; B02010004, 100V; Old Cat. No. BioAcc-Cool)
has to be used in combination with the Single Cycle Valve for
Water Cooler
(Cat. No. B02020004) to guarantee the
automatic temperature control of the sonication bath during the entire sonication process.
Magnetic ultrasound emitter maintenance
• The ultrasound waves are created from a series of magnets that are attached to the water tank. This system is very
sensitive to the heat generated during a run.
• Do not exceed 1 hour of total sonication per run. It is critical that the machine is allowed to cool at least 20 minutes
between runs. Damage resulting from non compliance to manual instructions will void the warranty and shorten the
lifespan of the machine.
• Ultrasound emitters can be damaged by tilting or jarring the machine. Exercise care if moving water tank.
Validated tubes for the Bioruptor® Pico
• DNA shearing: 0.1 ml (Cat.No. C30010015) and 0.65 ml (New Cat. No. C30010011; Old Cat. No. WA-005-0500)
Bioruptor®Microtubes for DNA shearing.
• Chromatin shearing: 0.1 ml (Cat. No. C30010015) and 1.5 ml (Cat.No. C30010016) Bioruptor®Microtubes and 15 ml
(Cat.No. C01020031) Bioruptor®Tubes & sonication beads.
Fitting 0.1, 0.65, 1.5, or 15 ml tubes in the tube holder
1. Place the tubes in the corresponding tube holder (0.1 ml tube holder & tube adaptor - Cat. No. B01200041,
0.65 ml tube holder – Cat. No. B01200043, 1.5 ml tube holder - Cat. No. B01200040 or 15 ml sonication accessories
- Cat. No. B01200016). Never leave empty spaces in the tube holder. Fill the empty spaces with tubes containing the
same volume of water. Screw the lid on the tube holder without over-tightening it.
2. Carefully place the tube holder on the holding plate.
3. During sonication, samples must remain at the bottom of the tube. If needed, briefly centrifuge samples during
sonication after pausing the run.
Bioruptor
