CORNING AXYGEN I-4010 Manual de usuario

Axygen®Microtube
Shaker
Instruction Manual
Catalog Numbers:
I-4010
I-4011
I-4012
A Corning Brand

Table of Contents
1.0 Safety Information .............................................................1
2.0 Design Environments ...........................................................2
3.0 Unpacking .....................................................................2
3.1 Package Contents ...........................................................2
3.2 Accessories (sold separately) ................................................3
4.0 Instrument Overview ...........................................................4
4.1 Control ....................................................................4
4.1 Display .....................................................................5
5.0 Instrument Operation ..........................................................5
5.1 Parameter Setting ..........................................................5
5.2 Start and Stop ..............................................................6
5.3 Running ....................................................................6
5.3.1 Single Step Work Without Saving.......................................6
5.3.2 Multiple Step Work Without Saving ....................................7
5.3.3 Program with Saving mode ............................................8
5.3.4 Load Program .........................................................8
5.3.5 Single Function Running ...............................................8
5.4 Temperature Calibration ....................................................9
5.5 Restore the Factory Settings ................................................10
6.0 Trial Run ......................................................................10
7.0 Troubleshooting...............................................................10
8.0 Maintenance and Cleaning ....................................................10
9.0 Limited Warranty..............................................................12
10.0 Equipment Disposal ...........................................................12

1
Instruction Manual
1.0 Safety Information
CAUTION: Risk of danger – there is material in the instruction manual which must
be read, understood, and followed to preserve safety operations and features.
Ensure that only trained staff or personnel work with the instrument.
CAUTION:
Caution during experimentation as the housing and the microtube shaker blocks
can be programmed to reach a temperature of 110°C. Refer to specifications.
Pay attention to the residual heat during block exchange and powering off –
risk of burn.
Protective ground contact
The socket must be grounded before each use.
When working wear personal protective equipment (PPE) to avoid the risk from:
– Splashing and evaporation of liquids
– Release of toxic or combustible gases
Set up the instrument on a level, stable, clean, non-slip, dry, and fire resistant
surface. Do not operate the instrument in explosive atmospheres, with
hazardous substances, or under water.
Always power off before fitting accessories.
Temperature must always be set at least 50°C lower than the fire point of the
media used.
Be aware of hazards due to:
– Flammable materials or media with a low boiling temperature
– Overfilling of media
– Incorrect use of accessories
Process pathogenic materials in closed vessels only.
Ensure the instrument and accessories comply with the safety and set up
instructions stated in this manual.
Do not use damaged components. Safe operation as described in this manual
is applicable with the accessories provided by Corning. Accessories must be
securely attached to the device.
The instrument can only be disconnected from the main power supply by
disconnection from the main or the connector plug. The main wall socket plug
is the preferred power disconnect method.
The voltage stated on the label must correspond to the main power supplied
from the region’s facility.
Ensure that the main power supply cord does not touch the microtube shaker.
Do not cover the device.
The instrument may only be opened or serviced by authorized service technicians.
The Axygen® Microtube shaker is for research use only.

2AXYGEN MICROTUBE SHAKER
2.0 Design Environments
The instrument is designed for the rapid heating, mixing, and cooling of biologic materials
and substrates used in research.
Observe the minimum distances between the devices, between the device and the wall and
above the assembly (min. 100 mm).
This device is not suitable for using in residential areas or other constraints mentioned in
Section 1.
>100 mm
Figure 1. Device placement.
3.0 Unpacking
Unpack the equipment carefully, and check for any damages which may have arisen during
transport before use. Please keep original packaging. Please contact the manufacturer/
supplier for technical support.
CAUTION: If there is any apparent damage to the system, please do not plug it
into the power line. Contact your local Corning support team.
3.1 Package Contents
Description Qty/Cs
Axygen® Microtube shaker 1
Power supply cords
I-4010 (120V)
I-4011 (230V)
I-4012 (230V)
1
1
1
Instruction manual 1
NOTE: Blocks purchased separately.

3
Instruction Manual
3.2 Accessories (sold separately)
Block Cat. No. Description
Speed
(rpm)
Well Dimensions
(diameter x depth)
I-4010-A Heating block, holds
0.5 mL tubes (24)
1,500 8.1 x 26 mm
I-4010-B Heating block, cone
bottom, holds 1.5
mL tubes (24)
1,500 10.9 x 30.8 mm
I-4010-C Heating block, round
bottom, holds 2 mL
tubes (24)
1,500 10.9 x 30.8 mm
I-4010-D Heating block, holds
5 mL tubes (8)
1,500 13.6 x 42 mm
I-4010-E Heating block, cone
bottom, holds 5 mL
tubes (8)
1,400 17 x 51.5 mm
I-4010-F Heating block, holds
15 mL tubes (8)
800 16.9 x 102.24 mm
I-4010-G Heating block, holds
50 mL tubes (4)
600 29.6 x 105.95 mm
I-4010-H Heating block
for 0.2 mL tubes
or 96-well PCR
microplates
1,500 –
I-4010-I Heating block for
96- or 384-well
microplates
1,500 –
– I-4010-TPC Block casing for
I-4010-A, -B, -C, -E
– –
– I-4010-PPC Block casing for
I-4010-H, -I
– –

4AXYGEN MICROTUBE SHAKER
4.0 Instrument Overview
4.1 Control
Block
Main unit
Adjustable knob
Function button
LCD screen
Vent holes
RS232 interface
Power interface
Power switch
Figure 2. Axygen® Microtube shaker overview.
Block Interchangeable magnetic attachment; ensure vessel type
matches block type.
LCD screen Display to set experiment parameters.
Function button Switches the input parameters or programming.
Adjustable knob At parameter setting state, rotate to alter function values. Press to
confirm setting. During operations press to pause/resume, press
and hold to stop/end.
RS232 interface Used for temperature calibration.
Power interface Connects the power cord.
Power switch Powers the instrument On/Off

5
Instruction Manual
4.1 Display
Set Displays the setting experiment parameters.
Actual Displays the actual parameters (ramp up/cool down).
Prog Program button, used for programming.
Temp Temperature will flash when selected.
Time Time will flash when selected. During Timed mode, the time will
countdown. In continuous operation mode, time will run for the
duration of operation (Figure 5).
Speed Speed of the setting area correlate to the 3 mm orbit agitation
motion in rpm and will flash when selected.
Adjustable knob At parameter setting state, rotate to alter function values. Press
to confirm setting. During operations press to pause/resume,
press and hold to stop/end.
5.0 Instrument Operation
5.1 Parameter Setting
Press the function button. The corresponding characters of the setting area will flash.
Rotate the parameter knob. Input the target parameters for the experiment.
Wait 3 seconds, the corresponding character will stop blinking. Complete parameter setting.
NOTE: When powered On, the Settings screen will display parameters of the last run. When
the Time is adjusted to 00:00, the device will run in continuous mode.

6AXYGEN MICROTUBE SHAKER
5.2 Start and Stop
Start: Complete the parameter input, push the knob to begin the protocol. Run indicates
the experiment is in progress.
Pause: Press the knob once to pause at any time during a Run.
In the Actual value column Pause will be displayed during a desired temporary pause in
the experiment. When in this state, only the Timer and Mixing functions are paused, the
Temperature Control function is still consistent with the temperature at the point of pausing.
Resume: Press the knob once to resume to the Run while in the Pause phase.
Stop: Press and hold the knob to stop the current Run.
5.3 Running
5.3.1 Single Step Work Without Saving
When powered On, the LCD screen shows the parameters of the last run, or factory settings.
Press the Temp, Speed, Time function buttons respectively, the corresponding characters will
flash. Rotate the knob to set each parameter according to the standard operating procedure
of the experiment. If Time is set to 00:00, it will run in continuous mode. After the setting is
completed, press the knob to start work.

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Instruction Manual
5.3.2 Multiple Step Work Without Saving
The program set in the single step is the first step by default.
Press the Prog function button to generate the second step. The last line in the Set column
now shows 1-2.
NOTE: Before setting the second step, there is one step by default. But after the Prog setting
is complete, the actual steps will be shown correctly once confirmed.

8AXYGEN MICROTUBE SHAKER
Complete N number of steps Then, press the knob to start work.
Prog shows the program and step number. Refer to the figure above, 2-2 refers to the total
number of steps and the current step of the run.
NOTE: Before the Time is set, the step will not take effect. If Time is set to 00:00, it will run in
continuous mode. Complete the current step input values prior to programming the next step.
5.3.3 Program with Saving mode
Follow programming practices as mentioned in 5.3.2 for Multiple Step procedures. After the
program has been built, press and hold, program name characters will flash (refer to the red
square in below figure). Rotate the knob to organize each program by a P1-P9 naming system.
Input the preferred P# of the newly generated program and keep for your records, then press
the knob to complete the input. The program saving is complete now.
Prog shows P1, 5-2, means program number 1 has been selected. P1 was saved with 5 steps
and currently working through Step 2.
5.3.4 Load Program
While powered On, press and hold the Prog button. This enters the operator into the Load
program mode. Rotate the knob to show the saved program, from P1 to the last, at most 9
programs. Switch to the program you want, and press the Prog button to check the program
details. Then, press the knob to start or run the program.
5.3.5 Single Function Running
If Temp is set to ---.-, it means working without temperature control function. When the Time
is adjusted to 00:00, the product will run in continuous mode. If speed is set to -----, it means
work without mixing function.
Este manual sirve para los siguientes modelos
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