Cleaver Scientific MultiSUB MSMINI Manual de usuario

Version 1

multiSUB® Electrophoresis Systems

MSMINI MSMIDI MSCHOICE MSCHOICEST MSMAXI

Instruction Manual

Catalogue No: MSMINI7, MSMINI10, MSMINIDUO

MSMIDI7, MSMIDI10, MSMIDIDUO

MSCHOICE7, MSCHOICE10, MSCHOICE15, MSCHOICETRIO

MSCHOICEST20, MSCHOICEST25

MSMAXI10, MSMAXI15, MSMAXI20, MSMAXIDUO, MSMAXI25

Page 2

Table of Contents

Page

Section 1 Safety Information 3

1.1 Safety Precaution 3

Section 2 General Information 4

2.1 Packing Lists 4

2.2 Care and Maintenance 7

Section 3 Operating Instructions 8

3.1 Usage Guidance and restrictions 8

3.2 Setting up the Horizontal Gel Tanks 9

3.3 Gel Preparation 10

3.4 Gel Pouring 11

3.5 Running the Gel 13

3.6 Gel Staining and Viewing 14

3.7 Solutions 14

Section 4 References 15

Section 5 Trouble Shooting Guide 16

Section 6 Product Information 17

6.1 Catalogue numbers and product descriptions for multiSUB® units & accessories 17

6.2 Combs 19

6.3 Catalogue numbers and product descriptions for CSL Related Products 21

Section 7 Warranty 22

Page 3

Section 1 Safety Information

1.1 Safety Precaution

When used correctly, these units pose no health risk. However, these units can deliver dangerous levels of

electricity and are to be operated only by qualified personnel following the guidelines laid out in this

instruction manual. Anyone intending to use this equipment should read the complete manual thoroughly.

The unit must never be used without the safety lid correctly in position. The unit should not be used if there is

any sign of damage to the external tank or lid.

These units comply with the following European directives:

2006/95/CE Low Voltage Directive and 2014/30/UE (official Title 2004/108/EC) EMC Electromagnetic

Compatibility

By virtue of the following harmonised standards:

BS EN IEC 61010-1: 2010 Safety Testing of Lab Equipment

BS EN IEC 61326-1:2013 EMC Electro Magnetic Compatibility

Page 4

Section 2 General Information

2.1 Packing Lists

MSMINI7, MSMINI10, MSMINIDUO

Units include tank, lid and electrodes and include the following accessories:

Tray

Tray Dams

Combs

Loading Guides

Cables

MSMINI7

MS7-UV7

7 x 7cm (W x L)

MS7-UVDAM

Pack of 2

2 x MS7-8-1

1mm, 8

sample

MS7-LG –Strips

MS7-WP –

Platform

CSL-CAB

MSMINI10

MS7-UV10

7 x 10cm (W x

MS7-UVDAM

Pack of 2

2 x MS7-8-1

1mm, 8

sample

MS7-LG –Strips

MS7-WP –

Platform

CSL-CAB

MSMINIDUO

MS7-UV7

MS7-UV10

MS7-UVDAM

Pack of 2

2 x MS7-8-1

1mm, 8

sample

MS7-LG –Strips

MS7-WP –

Platform

CSL-CAB

MSMIDI7, MSMIDI10, MSMIDIDUO

Units include tank, lid and electrodes and include the following accessories:

Tray

Tray Dams

Combs

Loading Guides

Cables

MSMIDI7

MS10-UV7

10 x 7cm (W x

MS10-

UVDAM

Pack of 2

2 x MS10-16-1

1mm, 16

sample

MS10-LG –Strips

MS10-WP –

Platform

CSL-CAB

MSMIDI10

MS10-UV10

10 x 10cm (W

x L)

MS10-

UVDAM

Pack of 2

2 x MS10-16-1

1mm, 16

sample

MS10-LG –Strips

MS10-WP –

Platform

CSL-CAB

MSMIDIDUO

MS10-UV7

MS10-UV10

MS10-

UVDAM

Pack of 2

2 x MS10-16-1

1mm, 16

sample

MS10-LG –Strips

MS10-WP –

Platform

CSL-CAB

Page 5

MSCHOICE7, MSCHOICE10, MSCHOICE15, MSCHOICETRIO

Units include tank, lid and electrodes and include the following accessories:

Tray

Tray Dams

Combs

Loading Guides

Cables

MSCHOICE7

MS15-UV7

15 x 7cm

(W x L)

MS15-

UVDAM

Pack of 2

2 x MS15-20-

1mm, 20

sample

MS15-LG –

Strips

MS15-WP –

Platform

CSL-CAB

MSCHOICE10

MS15-UV10

15 x 10cm

(W x L)

MS15-

UVDAM

Pack of 2

2 x MS15-20-

1mm, 20

sample

MS15-LG –

Strips

MS15-WP –

Platform

CSL-CAB

MSCHOICE15

MS15-UV15

15 x 15cm

(W x L)

MS15-

UVDAM

Pack of 2

2 x MS15-20-

1mm, 20

sample

MS15-LG –

Strips

MS15-WP –

Platform

CSL-CAB

MSCHOICETRIO

MS15-UV7

MS15-UV10

MS15-UV15

MS15-

UVDAM

Pack of 2

2 x MS15-20-

1mm, 20

sample

MS15-LG –

Strips

MS15-WP –

Platform

CSL-CAB

MSCHOICEST20, MSCHOICEST25

Units include tank, lid and electrodes and include the following accessories:

Tray

Dams

Combs

Loading Guides

Cables

MSCHOICEST20

MS15-

UVST20 15 x

20 cm

(W x L)

MS15-

UVDAM

Pack of 2

4 x MS15-

28MC-1

1mm thick, 28

sample MC

MS15-LG –

Strips

MS15-WP –

Platform

CSL-CAB

MSCHOICEST25

MS15-

UVST25 15 x

25 cm

(W x L)

MS15-

UVDAM

Pack of 2

4 x MS15-

28MC-1

1mm thick, 28

sample MC

MS15-LG –

Strips

MS15-WP –

Platform

CSL-CAB

Page 6

MSMAXI10, MSMAXI15, MSMAXI20, MSMAXIDUO, MSMAXI25

Units include tank, lid and electrodes and include the following accessories:

Tray

Dams

Combs

Loading Guides

Cables

MSMAXI10

MS20-UV10

20 x 10cm (W x

MS20-

UVDAM

Pack of 2

2 x MS20-20-

1mm, 20

sample

MS20-LG –Strips

MS20-WP –

Platform

CSL-CAB

MSMAX15

MS20-UV15

20 x 15cm (W x

MS20-

UVDAM

Pack of 2

2 x MS20-20-

1mm, 20

sample

MS20-LG –Strips

MS20-WP –

Platform

CSL-CAB

MSMAXI20

MS20-UV20

20 x 20cm (W x

MS20-

UVDAM

Pack of 2

2 x MS20-20-

1mm, 20

sample

MS20-LG –Strips

MS20-WP –

Platform

CSL-CAB

MSMAXIDUO

MS20-UV10

MS20-UV20

MS20-

UVDAM

Pack of 2

2 x MS20-20-

1mm, 20

sample

MS20-LG –Strips

MS20-WP –

Platform

CSL-CAB

MSMAXI25

MS20-UV25

20 x 25cm (W x

MS20-

UVDAM

Pack of 2

2 x MS20-20-

1mm, 20

sample

MS20-LG –Strips

MS20-WP –

Platform

CSL-CAB

Packing list checked by ________________________ Date ____________

The packing lists should be referred to as soon as the units are received to ensure that all components have

been included. The unit should be checked for damage when received.

Please contact your supplier if there are any problems or missing items.

Page 7

2.2 Care and Maintenance

Cleaning Horizontal Units

Units are best cleaned using warm water and a mild detergent. Water at temperatures above 600C can cause

damage to the unit and components.

The tank should be thoroughly rinsed with warm water or distilled water to prevent buildup of salts but care

should be taken not to damage the enclosed electrode and vigorous cleaning is not necessary or advised.

Air drying is preferably before use.

The units should only be cleaned with the following:

Warm water with a mild concentration of soap or other mild detergent.

Compatible detergents include dishwashing liquid, Hexane and Aliphatic hydrocarbons

The units should not be left to in detergents for more than 30 minutes.

The units should never come into contact with the following cleaning agents, these will cause irreversible

and accumulative damage:

Acetone, Phenol, Chloroform, Carbon tetrachloride, Methanol, Ethanol, Isopropyl alcohol

Alkalis.

Rnase Decontamination

This can be performed using the following protocol:

Clean the units with a mild detergent as described above.

Wash with 3% hydrogen peroxide (H2O2) for 10 minutes.

Rinsed with 0.1% DEPC- (diethyl pyro carbonate) treated distilled water,

Caution: DEPC is a suspected carcinogen. Always take the necessary precautions when using.

RNaseZAP™ (Ambion) can also be used. Please consult the instructions for use with acrylic gel tanks.

Page 8

Section 3 Operating Instructions

Further information (including videos) regarding setting up and running the multiSUB® units can be found at

www.cleaverscientific.com

3.1 Usage Guidance and restrictions

• Maximum altitude 2,000m.

•Temperature range between 4°C and 65°C.

• Maximum relative humidity 80% for temperatures up to 31OC decreasing linearly to 50% relative humidity at

40OC.

• Not for outdoor Use.

This apparatus is rated POLLUTION DEGREE 2 in accordance with IEC 664.

POLLUTION DEGREE 2, states that: “Normally only non-conductive pollution occurs.

Occasionally, however, a temporary conductivity caused by condensation must be expected”.

Page 9

3.2 Setting up the Horizontal Gel Tanks

Instructions for fitting Electrode Cables

1. Note the position of the lid on the unit. This shows the correct polarity and the correct orientation of the

cables, black is negative and red positive.

2. Remove the lid from the unit. Note if the lid is not removed, fitting the cables may result in un-tightening

of the gold plug and damage to the electrode.

3. Screw the cables into the tapped holes as fully as possible so that there is no gap between the lid and the

leading edge of the cable fitting.

4. Refit the lid.

Instructions for fitting Loading Guides

These can be fitted to enhance visibility of the wells if desired. They can be fitted to the white vinyl platform

sheet or to the unit itself.

1. Seat the tray in the unit and note the position of the comb grooves. The samples run black to red but the

trays can be used frontward or backwards so ensure that the comb grooves closest to the black electrode

are marked.

2. Remove the tray.

3. Peel the back off of the loading guide and carefully apply the loading guide directly to the gel platform.

The unit is now ready to be used.

Page 10

3.3 Gel Preparation

1. Table 1 below shows the volume of agarose solution required to make the desired agarose gel for each unit

tray size. For a standard 0.7% agarose gel, add 0.7 grams of agarose to 100 ml of 1x TAE or TBE solution.

The same 1 x solution should be used in the tank buffer solution.

Table 1

multiSUB® Mini

Tray

7 x 7 cm

7 x 10 cm

Gel volume for a 5mm thick gel

25 mL

35 mL

multiSUB® Midi

Tray

10 x 7 cm

10 x 10 cm

Gel volume for a 5mm thick gel

35 mL

50 mL

multiSUB® Choice

Tray

15 x 7 cm

15 x 10 cm

15 x 15 cm

Gel volume for a 5mm thick gel

52.5 mL

75 mL

112.5 mL

multiSUB® Choice

STRETCH

Tray

15 x 20 cm

15 x 25 cm

Gel volume for a 5mm thick gel

150 mL

187.5 mL

multiSUB® Maxi

Tray

20 x 10 cm

20 x 15 cm

20 x 20 cm

Gel volume for a 5mm thick gel

100 mL

150 mL

200 mL

2. Add the agarose powder to a conical flask.

3. Add the appropriate amount of 1x TAE or TBE solution from the table above. To prevent evaporation during

the dissolving steps below, the conical flask should be covered with parafilm.

4. Dissolve the agarose powder by heating the agarose either on a magnetic hot plate with stirring bar or in a

microwave oven. If using the microwave method, the microwave should be set at around a 400 watt or

medium setting and the flask swirled every minute. The solution should be heated until all crystals are

dissolved. This is best viewed against a light background. Crystals appear as translucent crystals. These will

interfere with sample migration if not completely dissolved.

The gel must be cooled to between 50°C and 60°C degrees before pouring.

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