Buchi B-820 Manual de usuario

BÜCHI Fat Determination B-820/B-821

Contents

5 Operation 28

5.1 Control, operating and display elements 28

5.2 Operating the unit 31

5.2.1 User configuration 34

5.2.2 Printer configuration 35

5.2.3 System configuration 36

5.2.4 Autosampler configuration 37

5.2.5 Preheating/Separation column conditioning 38

5.2.6 Gas flow rate setup 39

5.2.7 Sample weight and mode adjustment 43

5.2.8 Determination 45

5.2.9 Data output 49

5.2.10 Statistics 51

5.2.11 Clearing data 54

6 Maintenance 55

6.1 Control checks 55

6.2 Replacing the septum in the injection head 55

6.3 Cleaning the FID 56

6.4 Replacing the ignition coil 56

6.5 Checking the formic acid 57

6.6 Installing a new separation column 57

6.7 Changing the printer paper roll 57

6.8 Changing the ribbon 58

6.9 Checking for fractures and cracks 58

6.10 Customer service 58

6.11 Maintenance of the Autosampler 59

6.11.1 General 59

6.11.2 Syringe and syringe needle 59

6.11.3 Replacing the main power fuse 59

7 Taking out of operation 60

7.1 Switching off the unit 60

7.2 Storage/Transport 60

7.3 Disposal 60

8 Replacement parts 61

9 Appendix 62

9.1 Technical data 62

9.2 Error messages 63

9.3 Materials used 64

9.4 FCC requirements (for USA and Canada) 65

9.5 Declaration of conformity 66

1 Scope of delivery 3

2 Safety 7

2.1 Symbols 7

2.2 Requirements for the customer 7

2.3 Proper use 7

2.4 Improper use 8

2.5 General hazards 8

2.6 Safety measures 9

2.7 Handling of gas cylinders in the laboratory 10

2.8 Modifications 10

2.9 Responsibility of the operator 10

3 Function 11

3.1 Operational principles 12

3.2 System description Autosampler B-821 14

3.2.1 Introduction 14

3.2.2 Carousel support 14

3.2.3 Carousel 14

3.2.4 Turntable 14

4 Putting into operation 15

4.1 Accessories 15

4.2 Location of unit 15

4.3 Power connections 16

4.4 Connecting periphery devices 16

4.5 Filling the formic acid 18

4.6 Gas connections 18

4.7 Checking supply lines for leaks 20

4.8 Installing the separation column 21

4.9 Setting the correct gas flow rate 22

4.10 FID ignition 22

4.11 Installation of Autosampler B-821 22

4.11.1 Unpacking and inspection 22

4.11.2 Retrofitting a manual basic system with

an Autosampler 23

4.11.3 Installing the mounting plate 23

4.11.4 Mounting the Autosampler on the B-820 unit 23

4.11.5 Removing the transportation belt 23

4.11.6 Installing the syringe/needle assembly 24

4.11.7 Connecting the signal cable to the B-820 unit 24

4.11.8 Connecting power to the Autosampler B-821 24

4.11.9 Aligning the syringe needle with the injection

head of the B-820 unit 25

4.11.10 Connecting the pneumatics to the Autosampler 26

4.11.11 Installing the solvent reservoir bracket 26

4.11.12 Routing and securing the solvent lines and

the waste line 26

en, Version H (66 pages) Order no.

B-820/B-821 Instructions 96571

BÜCHI Fat Determination B-820/B-821

Please read these instructions thoroughly

before using the Fat Determination Unit

B-820/B-821. These instructions should

be kept near the unit for general reference.

Section 2 contains important notes on safety,

which must be observed if the Fat Determi-

nation Unit is to be operated safely.

Due to continuous improvement and updating, technical spe-

cifications may be altered without prior notice. No part of this

document may be reproduced, copied or transmitted in any

way nor by using electronic or optical systems without prior

agreement and written permission of Büchi Labortechnik AG.

BÜCHI Fat Determination B-820/B-821

1 Scope of delivery

Figure 1: Complete view of B-820/B-821 Figure 2: Complete view of B-820, Manual

Manual basic system

incl. individual

Version B-815 B-820 B-821 Total fat order no. fatty acids order no.

230V 230V 230V - 36925 37628

200V 120V 200V - 37109 37627

120V 120V 230V - 36924 37626

Automatic basic system

incl. individual

Version B-815 B-820 B-821 Total fat order no. fatty acids order no.

230V 230V 230V 230V 36923 37631

200V 120V 200V 230V 37108 37630

120V 120V 230V 230V 36922 37629

System prepared for Autosampler

incl. individual

Version B-815 B-820 B-821 Total fat order no. fatty acids order no.

230V 230V 230V - 37601 37634

200V 120V 200V - 37602 37633

120V 120V 230V - 37603 37632

Autosampler Retrofit 36926

Retrofit Fatty Acids manual 37635

Retrofit Fatty Acids automatic 37636

Table 1: Appliance models

BÜCHI Fat Determination B-820/B-821

Figure 3: Accessories

햳햲햴햵 햶햹햺

햷햻햾햿햸

1 Scope of delivery

Accessories Manual Basic System

Accessories B-815 (refer to Manual B-815)

Accessories B-820 Manual (refer to Table 2)

Accessories Automatic Basic System

Accessories B-815 (refer to Manual B-815)

Accessories B-820 Automatic (refer to Table 3)

Accessories Autosampler B-821 (refer to Table 4)

Accessories Autosampler Retrofit

Accessories Autosampler B-821 (refer to Table 4)

Accessories retrofit kit (refer to Table 5)

EPROM Set B-821

Accessories Individual Fatty Acids

Separation Column Fatty Acids 37620

Calibration Set Fatty Acids 37622

EPROM Set manual or automatic

Accessories B-820 Manual

1 Soap bubble meter, compl. 햶34878

1 Separation column "Total Fat" 햻34916

1 Supply line H2햳36134

1 Supply line Air 햲36135

1 Syringe 10µl 햾34258

1 High temperature septum (50 off) 햿34382

4 Vespel seals 햹34229

2 Threaded column joints 1/4" 햸34228

2 Fork spanners w.a. flats 8 햵16522

1 Fork spanner w.a. flats 17 x 19 햴19831

1 Power cable (PNE, 2.5 m)

available in the following versions: 햷

Type CH 10010

Type Grounded (shock-proof) 10016

Type GB 17835

Type AUS 17836

Type USA, 230 V 33763

1 Instruction manual available in the following languages:

German 96570

English 96571

French 96572

Italian 96573

Spanish 96574

1 Packaging 34560

Table 2: Accessories B-820 Manual

BÜCHI Fat Determination B-820/B-821

Accessories B-820 Automatic

1 Soap bubble meter, compl. 햶34878

1 Seperation column "Total Fat" 햻34916

1 Supply line H2햳36134

1 Supply line Air 햲36135

1 High temperature septum (50 pcs) 햿34382

4 Vespel seals 햹34229

2 Threaded column joints 1/4" 햸34228

2 Fork spanners w.a. flats 8 햵16522

1 Fork spanner w.a. flats 17 x 19 햴19831

1 T piece D1/8“ 36845

1 Jumper cord W VDE 30983

1 Power cable (PNE, 2.5 m)

available in the following versions 햷

Type CH 10010

Type Grounded (shock-proof) 10016

Type GB 17835

Type AUS 17836

Type USA, 230 V 33763

1 Instruction manual

available in the following languages:

German 96570

English 96571

French 96572

Italian 96573

Spanish 96574

1 Packaging 34560

Table 3: Accessories B-820 Automatic

1 Scope of delivery

BÜCHI Fat Determination B-820/B-821

Accessories B-821 Automatic

1 Septum *

1 Cylinder (reservoir) holder *

2 Glass cylinders for rinsing solvent and

waste 37069

1 Carousel 37085

1 Serial number label for carousel 37103

1 Mounting plate *

1 Sample vessel 2ml, compl. (100 ) 37068

4 Washers 16 mm *

2 Fuses 240V, 0.2A *

1 Fuse 250V, 0.5A *

1 Hamilton syringe 37066

1 Needle for Hamilton syringe 37067

1 Tubing PUR 1/8“ L = 3000 *

1 Foam rubber of neoprene length 100mm *

Table 4: Accessories Autosampler B-821

* Compare with spare parts list of the original operating instructions for

the 8200 CX.

Accessories Retrofit Kit

1 Cover for Autosampler 36758

1 Plate for Autosampler 36726

4 Spacers 36727

1 FID cap 34124

1 T piece D1/8“ 36845

2 LZS-K M4*10 10355

3 LZS-K M3*6 20390

3 FAE-SCH M3 10717

3 U-SCH M3 10571

4 SS-K M4*10 20909

1 Jumper cord W VDE 30983

5 Cable binders 2.6*200MM WS 03572

1 LZS-K M5*10 19645

Table 5: Accessories retrofit kit

1 Scope of delivery

BÜCHI Fat Determination B-820/B-821

2 Safety

This unit has been built in accordance with the state of

engineering and the recognized rules of safety.

Nevertheless, it can still be a source of risks and hazards:

• if used by persons with insufficient training

• if used for purposes other than that for which it is intended.

2.1 Symbols

Stop

Information about hazards which can lead to serious material

damage or cause serious or potentially fatal injury.

Warning

Information about hazards which can be harmful to the user’s

health or lead to material damage.

Please note

Information about technical requirements which if not observed

can lead to malfunctions, inefficiency and production loss.

2.2 Requirements for the customer

The device must only be operated by persons who have the

training or professional experience to comprehend the hazards

that can arise when using the unit.

Personnel without such training or persons currently under-

going a course of training should be trained thoroughly. This

Instruction Manual should be the basis for this training.

2.3 Proper use

The unit is designed and built for use in laboratories. It is intended

to be used for determining the fat content based on fatty

acids or the butyric acid. Its function depends on the Büchi

Extraction Unit B-815: Some microliters of the upper extracted

phase which contains the fatty acids and the internal standard

are injected either manually into the Fat Determination Unit

B-820, or automatically into the Fat Determination Unit with

Autosampler B-821. The detection occurs with a flame

ionization detector (FID). The subsequent evaluation is made

by integrating the total peak areas.

Only original Büchi replacement parts and accessories may

be used. Any modifications to the device or accessories shall

be permitted only upon consultation with and obtaining a

written authorization from the manufacturer.

2 Safety

BÜCHI Fat Determination B-820/B-821

2 Safety

2.4 Improper use

Any use other than those already listed, and any application

that does not conform with the technical data is deemed to

be improper use. The customer shall bear sole responsibility

for any damage resulting from improper use.

Use of this instrument is strictly prohibited in:

• explosion-proof environments,

• rooms where gas cylinders cannot be stored.

2.5 General hazards

General hazards arise from:

• covering the ventilation slots

• an insufficient distance from the back of the unit to the

wall (see Section 4 "Putting into operation“).

• touching the injection head and the FID (risk of burning).

• improper storage of gas cylinders.

• inadequate installation of connections to gas cylinders.

Apart from authorized maintenance personnel, no one is

allowed to remove any safety devices and covers.

Touching live parts may result in fatal injury!

Electrical danger

Before connecting the unit to power make sure that the opera-

ting voltage shown on the serial plate agrees with the mains

voltage.

The power plug may only be inserted into a socket

with grounded contact. Its protective effect may not

be suppressed by an extension line not having a

ground wire.

Warning:

a) Any interruption of the ground wire inside or outside of the

unit or a disconnection of the ground wire may cause

potential problems. Deliberate tampering is prohibited.

b) When opening covers or removing parts, unless this can

be done by hand, live parts may be exposed. Bear in mind

that connecting parts may also be live.

BÜCHI Fat Determination B-820/B-821

c) Before an alignment, maintenance, repair or replacement

of parts, the unit must be disconnected from all sources of

electric current, whenever it becomes necessary to open

the unit.

If any of the services above must be performed on a powered

on unit, it should only be done by a specialist who is famlilar

with the dangers involved.

d) It should be ensured that only fuses of the type and rated

amperage as indicated are used for replacement. The use

of repaired fuses or short-circuiting the fuse block is

prohibited.

e) If an operation free from danger cannot be ensured, the

unit must be put out of operation and protected from any

unauthorized use.

It can be assumed that an operation free from danger cannot

be ensured any more if:

• the unit shows visible damages,

• the unit does not work any more,

• after long-term storage under inadequate circumstances,

• after having been exposed to considerable strain during

transport.

2.6 Safety measures

The steel lines for hydrogen and synthetic air supplied with

the equipment have different connections to the unit preventing

improper connections.

Please make sure that connections at reduction valves are

correct.

If, due to a lack of gas pressure in the system, the electronic

valves will close preventing any hydrogen or synthetic air leaks.

Personal protective garments must be worn, such as goggles,

gloves and a laboratory coat.

The Instruction Manual is a component of the Fat Determi-

nation Unit B-820/B-821 and should remain with the unit for

easy reference by any user. This also applies to the additional

language variants of these instructions, which can be ordered

separately.

2 Safety

BÜCHI Fat Determination B-820/B-821

2.7 Handling of gas cylinders in the laboratory

Gas cylinders should be handled according to the pertinent

local regulations and provisions. The following are general

safety measures.

1. Protect gas cylinders from excessive heat, mechanical

damage and corrosive substances.

2. Gas cylinders should not be stored or connected in areas

with a major risk of fire.

3. Gas cylinders should be easily accessible at all times.

4. Full and empty gas cylinders should be stored separately

according to the type of gas.

5. Gas cylinders should only be stored and transported with

the protective cap screwed on.

6. Gas cylinders should be protected from falling down and

rolling away.

7. In case of leak and fire: Close cylinder valves immediately.

Cool any overheated cylinder with plenty of water.

8. Only keep as many replacement cylinders in the laboratory

as are necessary for continuous operation.

9. Open cylinder valves slowly.

10. Cylinder valves must neither be oiled nor greased.

11. Close cylinder valves when switching the unit off.

12. Close valves of empty cylinders.

2.8 Modifications

Modifications on the unit or on replacement parts or accessories

as well as the use of any other replacement parts or

accessories as those indicated in this Manual are only permitted

after consulting with Büchi Labortechnik AG and obtaining

written consent.

2.9 Responsibility of the operator

The operator is responsible for instructing personnel. For

manuals in other languages refer to Table 2, Section 1.

The operator should inform the manufacturer about events

relevant to safety which occur with operating the Fat Determi-

nation Unit.

2 Safety

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