Bioscreen C Pro Manual de usuario

Bioscreen C° ProTM

Operating Manual

Cat. No. 220021

Version 1.0.18

Oy Growth Curves Ab Ltd

2020

BioscreenC°ProTM

Bioscreen C° Pro is a photometric microplate

absorbance reader. Experiment protocols for the

Bioscreen C° Pro are created with BioScreener

PRO Software running on a computer connected

to the instrument. Experiment protocol controls

all the functions of the Bioscreen C° Pro

instrument. For a complete description of the

modes of operation, how to choose instrument

settings, etc. refer to the BioScreener PRO

Software User Guide.

Bioscreen C° Pro is used to measure absorbance

from sample liquids in special Honeycomb 2

Microplates. Incubation of the samples can be

carried out in a controlled temperature. The

instrument has three different shaking modes:

linear, orbital and double-orbital shaking.

This manual is designed to guide Bioscreen C° Pro

users through the basic hardware features of the

instrument. Although these instructions were

carefully written and checked, we cannot accept

responsibility for problems encountered when

using this manual. Oy Growth Curves Ab Ltd

reserves the right to make technical

improvements to this equipment and

documentation without prior notice as part of a

continuous program of product development.

This manual supersedes all previous editions.

Product manufactured by:

Oy Growth Curves Ab Ltd

Telekatu 12

FI-20360 Turku

Finland

www.bioscreen.fi

Oy Growth Curves Ab Ltd shall not be liable for

any damages whatsoever arising out of the use or

inability to use this product.

reserved. Oy Growth Curves Ab Ltd brand and

Bioscreen C° Pro and BioScreener PRO product

names are trademarks of Oy Growth Curves Ab

Ltd. Other brand and product names are

trademarks or registered trademarks of their

respective holders.

Table of Contents

1 Technical Specifications 4

2 Safety Information 5

2.1 Safety symbols and markings used on the

Bioscreen C° Pro instrument 5

2.2 General Information 5

2.3 Environmental Safety Standards 5

2.4 Electrical Safety 5

2.5 Photo biological Safety 5

2.6 Chemical and Biological Safety 6

2.7 Disposal of Packing Material and the

Instrument 6

2.7.1 Disposal of Transport Packing 6

2.7.2 WEEE Compliance 6

2.8 Cleaning and Decontamination of the

Instrument 6

2.8.1 Disinfection Steps 7

3 Intended Use 7

4 Instrument Overview 7

5 Installation 8

5.1 Instrument Installation 8

5.2 Software Installation 8

5.2.1 BioScreener PRO Software 8

5.2.2 Software Installation 9

5.3 Power and Communication Connections 9

5.3.1 Power Connection 9

5.3.2 Ethernet Communication Connection 10

5.3.3 Manual Setup for Direct Connection between the

Bioscreen C° Pro and the Computer 10

5.3.4 Connecting the Bioscreen C° Pro to a Local

Network with DHCP Server 12

5.3.5 Connecting the Bioscreen C° Pro to a Local

Network Manually 13

6 Device User Interface 14

6.1 Changing the Settings of the Instrument 14

7 Running an Experiment 17

7.1 Switching On 17

7.2 Installing the Thermo Cassette 18

7.3 Loading a Microplate 18

7.4 Opening the Cover during the

Experiment 20

7.5 Shutting Down the Instrument 20

8 Maintenance 21

8.1 Preventive and Regular Maintenance 21

8.2 How to Clean the Thermo Cassette 22

9 Service Protocols 23

9.1 Service Contracts 23

9.2 Service Request Protocol 23

9.3 How to Prepare for Service 23

Appendix A - Certificate of Decontamination 25

Appendix B - Guide to manual pipetting 26

1TechnicalSpecifications

Bioscreen C° Pro

Vertical filter based photometry

Light Source

Xenon flash lamp

Detector

Photodiode detector

Detection mode

Absorbance

Filters

Filter wheel with up to 8 filters

3 filters included as standard:

405 nm, 600 nm and Wide Band

filter (400-600 nm)

5 empty slots for extra filters purchased

(350, 420, 450, 492, 540, 580, 750 nm)

Optional filters on special request

Thermo Cassette

1-2 pcs simultaneous 100 well

Honeycomb 2 Microplates

Shaking

Linear, orbital and double-orbital shaking

patterns

User-selectable time, speed and

amplitude for each pattern

Incubation

Incubation range (in steps of 0.1°C):

Minimum: 9°C below ambient but

not under +15°C

Maximum: +65°C

Temperature accuracy +/- 0.1°C

Filter based Absorbance

Wavelength range: 350 - 800 nm

OD range: 0 to 4 OD (400 - 800 nm)

OD range: 0 to 3 OD (350 - 400 nm)

Computer Interface

Ethernet

Recommended PC Requirements

Operating System: Windows 10

Processor: Intel Core i5 or equivalent

Memory: 8GB DDR4

Display: 1920 x 1080

Storage: 500GB HDD

Power Requirements

External: 100-240 VAC, 50/60 Hz, max

input current 1.85A/115VAC, 1A/230VAC

Dimensions

Height: 44 cm (17.3 inches)

Width: 30 cm (11.8 inches)

Depth: 59,5 cm (23.4 inches)

Weight

26,6 kg (57.3 pounds)

Ambient conditions

Operating temperature: 5°C to 40°C

Transportation and storage temperature:

-25°C to 50°C

Humidity of atmosphere: 20% to 80%

Non-condensing

Instrument Conformity

Over voltage category II

Specifications are subject to change without

notice.

2SafetyInformation

2.1Safetysymbolsandmarkings

usedontheBioscreenC°Pro

instrument

Warning. Hot surface, risk of burns.

Warning. Optical radiation, risk of

injure to eyes or skin.

Manufacturer.

Date of manufacture.

Consult Instructions for use.

Do not treat this equipment as

unsorted municipal waste. Collect

waste electrical and electronic

equipment separately.

2.2GeneralInformation

This instrument is designed to provide full user

protection. When correctly installed, operated

and maintained it will present no hazard to the

user. Read this user manual entirely prior to

operating the instrument. Observe all Warning,

Caution and Note statements as well as safety

symbols and markings on the instrument and in

the documentation.

Warning:

This instrument must be installed and used as

outlined in this Operating Manual. Installation,

service and any operation which requires opening

of the instrument must be performed only by

trained and certified personnel from Oy Growth

Curves Ab Ltd or its representatives. Failure to

comply with these instructions will invalidate the

warranty and can lead to unsafe operation of this

equipment.

Warning:

The area designated for the instrument should be

free of dust, liquids and acidic vapor. The surface

of the table should be flat and even. Avoid areas

subject to vibrations and direct sunlight.

Warning:

Microplates operated at temperatures higher

than 55°C must cool down below 55°C before

being handled and removed from the instrument.

2.3EnvironmentalSafetyStandards

The environmental safety standards for operation

under norm IEC/EN 61010-1 and IEC/EN 61010-2-

010 are met under the following conditions:

Indoor use

Altitude: up to 2000 m

Temperature: +15°C to +35°C

Relative Humidity: maximum 80% at 31°C

non condensing then decreasing linearly

to 50% at 40°C

Mains supply voltage fluctuation: +/- 10%

from nominal

Overvoltage category: II (acc. to IEC

60364-4-443)

Pollution degree: 2 (acc. to IEC 61010-1)

2.4ElectricalSafety

Connect the unit only to a grounded

supply socket.

Connect the unit only to a power supply

with a designated voltage rating

corresponding to the label on the back of

the instrument.

2.5PhotobiologicalSafety

Xenon high power flash lamp light source is

incorporated and encapsulated in the Bioscreen

C° Pro instrument. The intended and routine use

of this instrument and its high power xenon flash

lamp does not pose a photo biological hazard,

given that the user strictly follows these

precautions:

Do not operate the instrument unless the

instrument shell is mounted and all

screws are in place and tightened.

Warning:

Xenon high power flash lamp emits ultraviolet

light during operation at levels that could injure

the eyes or skin. Do not look directly at the light

and avoid exposing any skin to the light. During

normal operation the user will not be exposed to

any light from the xenon flash lamp.

2.6ChemicalandBiologicalSafety

Ensure that the bottom of each

microplate is dry. Fluid on the bottom of a

microplate may present a contamination

hazard.

Wash your hands thoroughly after

handling test fluids.

Observe normal laboratory procedures

for handling potentially dangerous

samples.

Use proper protective clothing. Use

disposable gloves.

Ensure that the working area is well

ventilated.

Never spill fluids in or on the equipment.

Do not spill any alkaline onto any

instrument surfaces to avoid damage of

the instrument.

Do not autoclave any part of this

instrument.

Decontaminate the instrument

thoroughly before it is removed from the

laboratory or any servicing is performed

on it. (Follow decontamination

instructions in Chapter 2.8.)

Warning:

Use only mild detergent or 70% ethanol for

cleaning the instrument.

2.7DisposalofPackingMaterialand

theInstrument

The Bioscreen C° Pro is packed in a specially

designed carton. The carton is designed to assure

safe transport and to minimize transit damage.

Please save the original carton, the packing

materials and the pallet for future transportation.

If the unit should need to be returned for repair,

you must use the original packing materials and

carton for shipping.

2.7.1DisposalofTransportPacking

Straps to fix the packing on pallet, packaging

foam and plastic pallet must be disposed of and

recycled according to your local policy of plastic

recycling, if they can’t be saved and stored.

Transport cardboard box must be disposed of and

recycled according to your local policy of paper

recycling, if it can’t be saved and stored.

2.7.2WEEECompliance

The European Union Waste Electrical and

Electronic Equipment Directive (WEEE) is a

Producer Responsibility Directive aimed at

reducing the waste from electrical equipment,

increasing recovery and recycling rates of WEEE

and improving environmental performance of all

operators involved in the life cycle of electrical

and electronic equipment.

A product with WEEE symbol must be collected

separately according to the regulatory guidelines

of specific area or country.

2.8CleaningandDecontaminationof

theInstrument

If there is a risk of contamination with

biohazardous material, the procedure

recommended below or some other

corresponding decontamination procedure must

be performed. It is strongly recommended to

perform the complete decontamination

procedure before relocating the instrument from

one laboratory to another or shipping it to

service.

Warning:

The decontamination procedure should be

performed by authorized trained personnel in a

well-ventilated room wearing disposable gloves,

protective glasses and clothing.

Warning:

Working with decontamination agents may be

hazardous. Always observe the safety measures

given by the manufacturer of decontamination

agents. Use only agents specified in this manual.

Warning:

In case there is any doubt about the compatibility

of decontamination agents with parts of the

Bioscreen C° Pro or with materials being in

contact with the agent consult your local

Bioscreen C° Pro representative.

Use suitable disinfectants, e.g. Ethanol (70%).

2.8.1DisinfectionSteps

Basic procedure:

1. Prepare the decontaminant: ethanol 70%

or another agent recommended by your

safety officer.

2. Eject the plate carrier.

3. Take out the thermo cassette.

4. Disinfect the plate carrier and the door

mechanism below it.

5. Disinfect the thermo cassette and its

cover on both inside and outside using a

cloth dampened with 70% ethanol.

6. If you are shipping the device for service,

leave the thermos cassette outside the

device for packaging. Otherwise install

the thermo cassette back in the plate

carrier.

7. Close the door.

8. Switch OFF the power and disconnect the

power cable.

9. Disinfect the outside of the instrument

using a cloth dampened with 70%

ethanol.

10. After performing this decontamination

procedure, enclose a signed and dated

Certificate of Decontamination (Appendix

A, page 25) both inside the possible

transport package and attached to the

outside of the package.

3IntendedUse

The Bioscreen C° Pro photometric microplate

absorbance reader is intended for research or

routine-test laboratories to be used by

professional personnel, who understand the

nature of photometry. The Bioscreen C° Pro is a

microplate photometer for measuring

absorbance from samples in Honeycomb 2

Microplates in 100-well (10x10) plate format. It

also has incubation and shaking capabilities.

Note:

The instrument may only be used for research

and development or for other non-clinical

purposes.

If the assay performance is essential for the

analysis, the test result has to be ensured with

internal quality controls or with an alternative

test. For validation of the entire system, it is

recommended that Good Laboratory Practices

(GLP) are followed to guarantee reliable analyses.

4InstrumentOverview

Figure 4-1: Bioscreen C° Pro front view.

Figure 4-2: Bioscreen C° Pro back view.

5Installation

5.1InstrumentInstallation

Note:

When moving the Bioscreen C° Pro, it is

recommended that two people lift the instrument

together to avoid personal injury. Be sure to lift

the instrument from the metal frame at the

bottom to avoid injury and/or damage to the

instrument. Always lift from the metal frame only.

Lifting from the white plastic covers is not

allowed as the plastic parts will break.

Note:

Unpack the instrument according to printed

Bioscreen C° Pro Quick Guide which you can find

inside the shipping carton.

When unpacking the instrument, please check to

ensure that all of the following parts are included:

1. Bioscreen C° Pro Instrument

2. Bioscreen Memory Stick

Bioscreen C° Pro Operating Manual

BioScreener PRO SW User Guide

Bioscreen C° Pro Quick Guide

BioScreener PRO Software

3. Thermo Cassette

4. Power Adapter

5. Power Cable

6. Ethernet Cable

7. Honeycomb Plates (4 pcs)

8. Bioscreen C° Pro Quick Guide

Contact your Oy Growth Curves Ab Ltd

representative immediately if any of these items

are missing.

The area designated for the instrument should be

free of dust, liquids and acidic vapors. The table's

surface should be flat and even. Avoid areas

subject to vibrations and direct sunlight.

A minimum of 10 centimeters of open space is

required on both sides of the instrument. Space

in front of the instrument is required to access

the measurement chamber door.

After unpacking and positioning the instrument,

please do the following steps in the given order:

1. Install the BioScreener PRO software.

2. Plug in power and Ethernet cable.

3. Check settings for Bioscreen C° Pro with

the device user interface (refer to

Chapter 5.3.5).

Follow the instructions in the next chapters.

5.2SoftwareInstallation

5.2.1BioScreenerPROSoftware

BioScreener PRO Software that is installed on a

local computer is needed to access Bioscreen C°

Pro instrument. Software is used to create

experiment protocols and upload them to the

Bioscreen C° Pro. After the experiment run is

completed software is used to download the

results from the Bioscreen C° Pro to the computer

for further processing.

Note:

BioScreener PRO Software is compatible with

Windows 10 operating system.

5.2.2SoftwareInstallation

1. Remove the Bioscreen USB memory stick

from the shipping box:

2. Rotate the tail of the USB stick to release

the connector:

3. Insert the USB stick to a USB port of the

computer you want to use to run the

Bioscreen C° Pro instrument.

4. Double-click the software installer (the

file that has .msi file type) in the window

that opens:

Note:

Numbers in parentheses indicates the version

number of the software.

5. Click “Yes” to the window that possibly

opens:

6. The software is installed automatically

and a BioScreener PRO icon appears into

a desktop of your computer:

7. Start the software by double-clicking the

icon.

5.3PowerandCommunication

Connections

5.3.1PowerConnection

Inspect the voltage information on the power

adapter to ensure that it corresponds to the local

main power specifications. Also make sure the

power cable is grounded. Hereafter, the power

cable can be connected to the instrument.

Note:

To prevent power spikes, always connect the

power cable to the Bioscreen C° Pro first and only

after that connect the power adapter to main

power supply.

Warning:

Bioscreen C° Pro instrument must always be used

with the original power adapter only.

Figure 5-1: How to connect the power cable.

Figure 5-2: How to connect the power adapter.

Note:

Whenever disconnecting the power cable, please

note that the connector is equipped with a cable

strain relief, and must only be detached by

releasing the lock, sliding the connector mantle

backwards.

5.3.2EthernetCommunicationConnection

Connect the RJ45 Ethernet cable between the

Bioscreen C° Pro and local Ethernet network

(directly to computer or via network router based

on local policies).

Figure 5-3: How to connect the Ethernet cable.

Network settings for the Bioscreen C° Pro are

either entered manually via device user interface

(default) or collected automatically from a DHCP

server.

Note:

If you are unsure which to use, contact your local

IT support for help.

Communication between the Bioscreen C° Pro

and the software is done with normal TCP/IP

protocol. Default connection method is a direct

point-to-point connection between the Bioscreen

C° Pro instrument and the computer. This works

well in environments where the computer does

not need access to other local network resources.

It is also possible to connect the Bioscreen C° Pro

to a local network. In this case the IP settings

need to be set to comply with local network

policies. Consult your local IT specialist for

network details prior connecting the Bioscreen C°

Pro to local network.

5.3.3ManualSetupforDirectConnection

betweentheBioscreenC°Proandthe

Computer

By default, Bioscreen C° Pro is set for direct point-

to-point connection with your computer. If you

are using this connection method, you will only

need to configure your computer’s network

settings to comply with Bioscreen C° Pro’s.

Default IP address for Bioscreen C° Pro is

192.168.1.10

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