Biomeme Franklin Manual de usuario

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Franklin™

Real-Time PCR

Thermocycler and

Biomeme Go App 

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Last Updated: 03/13/2020

Version: 1.0

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 2
Table of Contents
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Brief Overview 4
Thermocycler Technical Specifications 5
Thermocycler Button Layout 7
Logging In and Out of Biomeme Go 8
Loading Sample into Go-Strips 8
Placing into Franklin™ Thermocycler 9
How To Start Your First Run 10
During the Run 13
How To Create a New Project Folder 13
How To Create a New Protocol 14
How To Delete a Protocol 14
Interpreting Results 15
Viewing Completed Run Data 19
Viewing Incomplete Runs 19
Thermocycler LED Status Indicators 20
Turning Your Thermocycler On and Off 21
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Franklin™ PCR Thermocycler and Biomeme Go
User Manual
© Biomeme, Inc. 2020
Version 1.0
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 3
Charging & Checking Thermocycler Battery Status 21
Enabling & Disabling Bluetooth (BLE ) on Your Thermocycler 23
How To Update the  Biomeme Go App 23
How To Run a Melt Curve 24
Transferring Data 25
Wireless (Bluetooth) 25
Reporting a Problem or Error 25
Viewing Thermocycler Device Information 26
Switching Teams (Enterprise Plan Only) 26
Maintenance & Cleaning 27
Safety Notice 28
General Safety Warnings 28
Declaration of Conformity 30
Troubleshooting 31
Disclaimer 38
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
Franklin™ PCR Thermocycler and Biomeme Go
User Manual
© Biomeme, Inc. 2020
Version 1.0




4



Brief Overview



The Biomeme Franklin™ transforms your smartphone into a thermocycler for

real-time PCR or isothermal analysis with sample-to-result in 30-60 minutes,

depending on the test protocol. Our mobile thermocycler enables multiplex

real-time detection of up to 27 targets from 1 sample or test 9 samples for up to 3

targets each. Just under 3 lbs, hand-held, and battery-operated for maximum

portability enabling a full day’s work out in the field on a single charge. Franklin™

comes in 3 different variations depending on the color channel detection you

require (see Technical Specifications



for details).



Franklin™ PCR Thermocycler and Biomeme Go

User Manual

Version 1.0

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5

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Thermocycler Technical

Specifications

SPECIFICATION

VALUE

Sample Capacity

9 Wells

Reaction Volume per Well

20µL

Total Channels

3

Franklin™ one9 Fluorophore*

FAM / SYBR (Green)

Franklin™ two9 Fluorophores*

FAM / SYBR (Green), ATTO647N (Red)

Franklin™ three9 Fluorophores

FAM / SYBR (Green), TexasRedX (Amber),

ATTO647N (Red)

System Control & Data Transfer

Wireless (BLE)

Integrated Barcode Scanner

Yes

Max Samples per Run

9

Max PCR Targets per Run

27

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Franklin™ PCR Thermocycler and Biomeme Go

User Manual

Version 1.0

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6

Weight

1.20 kg / 2.65 lb

Operating Ambient Temperature

4 - 40°C / 39 - 104°F

Operating Humidity Limit

0 - 99%

Operating Altitude Limit

3,048 m / 10,000 ft

Wall Power (VAC)

100 - 240V

Voltage

19V

Full Load Current

3.3A

Internal Battery

5 hrs

Quantitative

Yes

IP Rating

IP30

Indoor/Outdoor?

Indoor or Outdoor in a Covered Area

Pollution Degree

2



Franklin™ PCR Thermocycler and Biomeme Go

User Manual

Version 1.0



7

Degree of Ingress Protection

Keep 5 cm Clearance Around the

Thermocycler for Proper Performance

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Note:



The Franklin™ one9 is limited to the FAM/SYBR (Green) channel and the two9 is limited to the FAM/SYBR (Green)

and ATTO647N (Red) channels only. You can



upgrade your thermocycler(s)



at any time without returning your device.

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Thermocycler Button Layout

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There are a total of 4 buttons located on the top of your Franklin™ thermocylcer:

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Franklin™ PCR Thermocycler and Biomeme Go

User Manual

Version 1.0



8

Logging In and Out of Biomeme Go

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To log in (Requires internet connection)

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1. Open the Biomeme Go mobile app on your smartphone by tapping the app

icon on your phone’s home screen.

2. Enter your email address and password (both are case sensitive).

3. Tap the Login button.

4. If you’re part of multiple teams (Enterprise Users Only), select which team

to log in under (you can toggle between teams once logged in).

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Note:

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If you have forgotten your password, click “Forgot Password” or email



[email protected]



. If you

are traveling to a remote location to perform PCR and will not have internet access while there, you must log

in before your connection is lost.

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To log out

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1. Open the User menu by clicking the user icon in the top left corner.

2. Select Logout.



Loading Sample into Go-Strips

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Attention: Contents of the Go-Strip may shift during transport. When starting to

work with your test, make sure the cake of the lyophilized reagent rests at the

bottom of the Go-Strip wells. Tap the bottom of the sealed Go-Strip gently but

firmly against a solid surface before removing the foil seal and adding your sample.

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Franklin™ PCR Thermocycler and Biomeme Go

User Manual

Version 1.0

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9

●Tear open the foil pouch to retrieve your Go-Strip, but do not discard the foil

pouch as you’ll need to scan the QR code.

●Remove the foil seal from your Go-Strip, and transfer 20µL of purified sample

into each well of your Go-Strip.

●Once all wells of your Go-Strip are filled, place a void filling cap into the strip.

●Align the Go-Strip and void filling cap so that the strip connections are visible

through the cap cutouts as shown in the illustration below.

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Placing into Franklin™ Thermocycler

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●Open the lid of your thermocycler by pressing the latch on top of the unit.

●Place your Go-Strip, with the void filling cap inserted, into a 3-well slot. Don’t

worry if the void filling cap feels slightly loose. When the lid of the

thermocycler is closed, it will secure the caps into place, sealing the PCR

reaction.

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Franklin™ PCR Thermocycler and Biomeme Go

User Manual

Version 1.0

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10

●It’s important to make sure your Go-Strip is oriented correctly when placing

it into your thermocycler. Make sure the strip connections that are visible

through the void filling cap cutouts are facing the back of your thermocycler

as shown in the illustration below, then close the lid.

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●Navigate to the Biomeme Go mobile application on your smartphone to

begin your testing protocol. For further instructional information, please

contact [email protected].



Note:

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Transport your Franklin™ thermocycler in its carrying case. Additionally, moving your thermocycler while

thermocycling could result in errors. We highly recommend not moving or opening the device while thermocycling to

avoid losing your PCR run. After your run has completed, be careful when removing your Go-Strips and void filling caps

to avoid liquid splatter.

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How To Start Your First Run

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1. Grab your smartphone and log into the Biomeme Go app if you haven’t

done so already (see “Logging In and Out”).

2. From the main dashboard of Biomeme Go, tap Start Run.

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Franklin™ PCR Thermocycler and Biomeme Go

User Manual

Version 1.0



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