Biohit Proline Manual de usuario

Proline®

Käyttöohje

Bruksanvisning

Instruction Manual

Käyttöohje ....................................................1

Bruksanvisning .......................................... 17

Instruction Manual .................................... 33

Specifications ............................................ 48

Proline®

Grip cover:

metal blue 721060

grey 721752

yellow 721825

red 721706

blue 721221

green 721792

Handle,

complete, in gray colour

0.1-2.5 µl 721061

0.5-10 µl 721062

2-20 µl 721063

5-50 µl 721064

10-100 µl 721065

20-200 µl 721082

50-200 µl 721066

100-1000 µl 721067

200-1000 µl 721068

1-5 ml 721069

Piston assembly:

0.1-2.5 µl 721145

0.5-10 µl 721141

2-20 µl 721150

5-50 µl 721150

10-100 µl 721160

20-200 µl 721160

50-200 µl 721160

100-1000 µl 721175

200-1000 µl 721175

1-5 mI 721173

Piston:

0.1-2.5 µl 721146

0.5-10 µl 721142

2-20 µl 721151

5-50 µl 721151

10-100 µl 721161

20-200 µl 721161

50-200 µl 721161

100-1000 µl 721176

200-1000 µl 721176

1-5 ml 721177

Piston spring:

0.1-2.5 µl 721143

0.5-10 µl 721143

2-20 µl 721152

5-50 µl, 721152

10-100 µl 721162

20-200 µl 721162

50-200 µl 721162

100-1000 µl 721465

200-1000 µl 721465

1-5 ml N/A

Spring support:

0.1-2.5 µl 721144

0.5-10 µl 721144

2-20 µl N/A

5-50 µl N/A

10-100 µl 721144

20-200 µl 721144

50-200 µl 721144

100-1000 µl 721464

200-1000 µl 721464

1-5 mI N/A

O-ring, set of 10 pieces

0.1-2.5 µl N/A

0.5-10 µl 721090

2-20 µl 721100

5-50 µl 721100

10-100 µl 721110

20-200 µl 721110

50-200 µl 721110

100-1000 µl 721120

200-1000 µl 721120

1-5 ml 721121

Secondary spring:

0.1-2.5 µl N/A

0.5-10 µl N/A

2-20 µl N/A

5-50 µl N/A

10-100 µl N/A

20-200 µl N/A

50-200 µl N/A

100-1000 µl 721463

200-1000 µl 721463

1-5 mI N/A

Tip cone:

0.1-2.5 µl 721012

0.5-10 µl 721011

2-20 µl 721020

5-50 µl 721020

10-100 µl 721030

20-200 µl 721030

50-200 µl 721030

100-1000 µl 721041

200-1000 µl 721041

1-5 mI 721047

Tip cone filter (pack of 50 pieces)

Standard Plus

0.1-2.5 µl N/A N/A

0.5-10 µl N/A N/A

2-20 µl 721008 721018

5-50 µl 721008 721018

10-100 µl 721007 721017

20-200 µl 721007 721017

50-200 µl 721007 721017

100-1000 µl 721006 721016

200-1000 µl 721006 721016

1-5 ml 721005 721015

Tip ejector collar:

0.1-2.5 µl 721053 (metal blue)

0.5-10 µl for 300 µl tip 721054 (metal blue)

0.5-10 µl for 300 µl tip 721755 (grey)

0.5-10 µl for 10 µl tip 721053 (metal blue)

0.5-10 µl for 10 µl tip 721754 (grey)

2-20 µl 721054 (metal blue)

5-50 µl 721054 (metal blue)

5-50 µl 721700 (red)

10-100 µl 721054 (metal blue)

20-200 µl 721054 (metal blue)

50-200 µl 721054 (metal blue)

50-200 µl 721806 (yellow)

100-1000 µl 721055 (metal blue)

200-1000 µl 721055 (metal blue)

200-1000 µl 721080 (blue)

1-5 mI 721056 (metal blue)

1-5 mI 721797 (green)

Tip ejector:

metal blue, 721057

grey, 721753

yellow, 721826

red, 721704

blue, 721230

green, 721793

Tip ejector spring:

721240

Push button:

metal blue, 721059

grey, 721751

white, 721223

yellow, 721824

red, 721703

blue, 721220

green, 721791

Counter:

0.1-2.5 µl 721185

0.5-10 µl 721182

2-20 µl 721192

5-50 µl 721191

10-100 µl 721202

20-200 µl 721201

50-200 µl 721201

100-1000 µl 721212

200-1000 µl 721212

1-5 mI 721213

N/A= Not Available

Grip cover:

metal blue, 721060

grey, 721752

yellow, 721825

red, 721706

blue, 721221

Handle, complete,

in gray colour:

4-channel:

5-50 µl 721070

50-250 µl 721071

8-channel:

0.5-10 µl 721072

5-50 µl 721073

50-300 µl 721075

12-channel:

0.5-10 µl 721076

5-50 µl 721077

50-300 µl 721079

Piston assembly:

0.5-10 µl 721276

5-50 µl 721271

50-250 µl 721272

50-300 µl 721278

Tip cone housing,

complete, in gray colour:

4-channel, 721677

8-channel, 721668

8-channel, 0.5-10 µl 721667

12-channel 721676

12-channel, 0.5-10 µl 721675

Tip ejector bar:

4-channel 721268

8-channel 721269

8-channel, 0.5-10 µl 721280

12-channel 721270

12-channel, 0.5-10 µl 721666

NOTE:

Coloured spare parts presented in

these two pages are for the metal

blue/gray Biohit Proline pipettors.

Spare part codes for the white

handles and tip cone housings are

in a separate spare part list, which

can be ordered from Biohit

subsidiaries or distributors.

Push button:

metal blue, 721059

grey, 721751

yellow, 721824

red, 721703

blue, 721220

Tip ejector, complete:

metal blue, 721058

grey, 721758

yellow, 721862

red, 721828

blue, 721231

Tip ejector spring:

721240

Tip cone:

0.5-10 µl 721275

5-50 µl 721273

50-250 µl 721274

50-300 µl 721277

Tip cone filter (set of 50 pcs):

0.5-10 µl N/A

5-50 µl 721014

50-300 µl 721014

Piston:

0.5-10 µl 721296

5-50 µl 721297

50-250 µl 721298

50-300 µl 721298

Piston spring:

0.5-10 µl 721261

5-50 µl 721261

50-250 µl 721261

50-300 µl 721261

Spring support:

0.5-10 µl 721262

5-50 µl 721262

50-250 µl 721262

50-300 µl 721262

Elastomer:

0.5-10 µl 721300

CONTENTS

1 . Your new Biohit Proline pipettor ..................................................... 34

1.1. Biohit Proline adjustable volume pipettors...................................34

1.2. Biohit Proline fixed volume pipettors ...........................................35

1.3. H-sign (Konformitätsbescheinigung) ............................................35

1.4. Biohit Proline tips ........................................................................ 36

2. Unpacking ......................................................................................... 37

3. Installing the pipettor holder ........................................................... 37

4. Pipettor materials ............................................................................ 38

5. Operating the pipettor ..................................................................... 39

5.1. Volume setting ............................................................................. 39

5.2. Sealing and ejecting tips ............................................................. 39

5.3. Two-in-one pipettors ................................................................... 40

5.4. Protective filters .......................................................................... 40

6. Pipetting techniques ........................................................................ 41

6.1. Forward pipetting ........................................................................ 41

6.2. Reverse pipetting ........................................................................ 42

7. Storage .............................................................................................. 42

8. Testing the performance and recalibration ................................... 42

8.1. Performance test (Checking calibration)...................................... 42

8.2. Recalibration procedure .............................................................. 44

9. Maintenance ......................................................................................44

9.1. Cleaning and decontaminating your pipettor ..............................44

9.2. In-house maintenance ................................................................. 45

10. Troubleshooting ................................................................................ 46

11. Warranty information ......................................................................... 47

1. Your new Biohit Proline pipettor

Your new Biohit Proline hand-held pipettor is a general purpose

pipettor, for the accurate and precise sampling and dispensing of liquid

volumes. All Biohit Proline pipettors operate on the basis of the air

displacement principle and use disposable tips.

The range of Biohit Proline pipettors cover a volume range from 0.1 µl

to 5 ml.

1.1. Blohit Proline adjustable volume pipettors

Single channel pipettors:

Multichannel pipettors:

Safe-Cone

Filters

50 pcs/bag

Cat.No. Volume Range Increment Tip Standard

720120 4-ch 5-50 µl 0.5 µl 300, 350 µl, 721014

350 µl 4 tip-band

720130 4-ch 50-250 µl 5 µl 300, 350 µl, 721014

350 µl 4 tip-band

720210 8-ch 0.5-10 µl 0.1 µl 10 µl -

720220 8-ch 5-50 µl 0.5 µl 300, 350 µl, 721014

350 µl 4 tip-band

720240 8-ch 50-300 µl 5 µl 350 µl, 721014

350 µl 4 tip-band

720310 12-ch 0.5-10 µl 0.1 µl 10 µl -

720320 12-ch 5-50 µl 0.5 µl 300, 350 µl, 721014

350 µl 4 tip-band

720340 12-ch 50-300 µl 5 µl 350 µl, 721014

350 µl 4 tip-band

Safe-Cone Filters

Volume 50 pcs/bag

Cat.No. Range Increment Tip Standard Plus

720005 0.1-2.5 µl 0.05 µl 10 µl - -

720000 0.5-10 µl 0.1 µl 10, 300 µl - -

720080 2-20 µl 0.5 µl 300 µl 721008 721018

720020 5-50 µl 0.5 µl 300, 350 µl 721008 721018

720050 10-100 µl 1 µl 300, 350 µl 721007 721017

720070 20-200 µl 1 µl 300, 350 µl 721007 721017

720030 50-200 µl 1 µl 300, 350 µl 721007 721017

720060 100-1000 µl 5 µl 1000 µl 721006 721016

720040 200-1000 µl 5 µl 1000 µl 721006 721016

720110 1000-5000 µl 50 µl 5000 µl 721005 721015

1.2. Biohit Proline fixed volume pipettors

Single channel pipettors:

1.3. H-sign (Konformitätsbescheinigung)

All Biohit Proline pipettors have been quality tested according to ISO

8655-6 and have received the H-sign (Konformitätsbescheinigung, DIN

12600) printed on the grip of each pipettor. The quality control

according to ISO 8655 involves gravimetric testing of each pipettor with

distilled water (grade 3, ISO 3696) at 22OC using the manufacturer’s

original tips.

Safe-Cone Filters

Volume 50 pcs/bag

Cat.No. Range Tip Standard Plus

722001 5 µl 10, 300, 350 µl - -

722004 10 µl 10, 300, 350 µl - -

722010 20 µl 300, 350 µl 721008 721018

722015 25 µl 300, 350 µl 721008 721018

722020 50 µl 300, 350 µl 721008 721018

722025 100 µl 300, 350 µl 721007 721017

722030 200 µl 300, 350 µl 721007 721017

722035 250 µl 1000 µl 721006 721016

722040 500 µl 1000 µl 721006 721016

722045 1000 µl 1000 µl 721006 721016

722050 2000 µl 5000 µl 721005 721015

722055 5000 µl 5000 µl 721005 721015

1.4. Biohit Proline tips

The full range of Biohit pipettor tips are recommended for use with

Biohit Proline pipettors. Biohit standard tips are made of virgin

polypropylene. Biohit also offers a full range of filter tips. Biohit

standard tips are available as bulk packages, space saving refill

systems and autoclavable (121°C, 20 min, 1 atm) trays. Pre-sterilized

tips in trays are also available. (Fig. 1.)

Fig. 1.

2. Unpacking

The Biohit Proline pipettor package contains the following items:

1. Pipettor

2. Calibration/Opening tool

3. Grease

4. Instructions for use

5. Pipettor holder

6. Tip

7. Identification stickers

8. Caps for colour coding (excludes fixed volume pipettors)

9. Filters (pipettors > 10 µl)

10. Performance certificate according to ISO 8655-6

3. Installing the pipettor holder

For convenience and safety always keep the pipettor vertically on its

own holder when not in use. When installing the holder, please follow

the instructions below:

1. Clean the shelf surface with ethanol.

2. Remove the protective paper from the adhesive tape

3. Install the holder as described in Figure 2A.

(Make sure the holder is pressed against the edge of the shelf.)

4. Place the pipettor onto the holder as shown in Figure 2B.

Fig. 2A

Fig. 3.

Fig. 2B

Note: An ergonomic, convenient carousel stand for 5 Biohit Proline

pipettors is also available (Cat. No.721000) (Fig. 3).

4. Pipettor Materials

Biohit Proline Single Channel

Biohit Proline Multichannel

Push button Push button

rod (ABS) (Operating button)

(PA)

Grip Cover

(PA) Tip ejector

(PA)

Handle

(ABS)

Display cover

(PC)

Tip Ejector

collar (PA)

Tip cone

2.5–1000 µl (PVDF)

2000–5000 µl (PPS)

Push button Push button

rod (ABS) (PA)

Grip cover

(PA) Tip Ejector

(PA)

Handle

(ABS)

Display cover

(PC)

Tip cone

housing

(ASA/PC)

Tip cone,

soft sealing

Tip ejector (PVDF)

bar (PETP) (TPU)

(PVDF)

Hard tip

cone

(PC)

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