Biohit ELINE Dispenser Pro Manual de usuario

User Manual

Bedienungsanleitung

Mode d'emploi

Manual Usuario

Instruzioni d'impiego

eLINE®Dispenser Pro

User Manual ............................................................1

Bedienungsanleitung .......................................... 21

Mode d’ emploi..................................................... 41

Manual Usario ....................................................... 61

Instruzioni d’impiego .......................................... 81

Specifications ......................................................101

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eLINE®Dispenser Pro

Contents

1. Intended use .......................................................................................................................................2

2. Biohit eLINE Dispenser Pro............................................................................................................2

3. Quick start...........................................................................................................................................3

3.1. Unpacking.............................................................................................................................3

3.2. Charging the Biohit eLINE Dispenser Pro before first use..................................4

3.3. Biohit eLINE Dispenser Pro operating instructions ...............................................5

3.3.1. Display....................................................................................................................................6

3.3.2. Keyboard...............................................................................................................................7

3.3.3. Reset Button........................................................................................................................7

3.3.4. Start Button.........................................................................................................................8

3.3.5 Tip Attachment and Ejection....................................................................................... 8

4. Charging of the Biohit eLINE Dispenser Pro ...........................................................................8

4.1.

Charging with the Charging Stand and Charging Carousel ............................... 9

4.2. Charging through direct charging system ............................................................ 10

4.3. Charging Recommentations ........................................................................................11

4.4. Electrical specifications.................................................................................................11

5. Programming principles ...............................................................................................................11

5.1. Programming map.......................................................................................................... 13

5.2. Main Modes ...................................................................................................................... 14

5.2.1. d-mode, Programming using TipGuide................................................................... 14

5.2.2. dST-mode........................................................................................................................... 14

5.3. OPT-mode.......................................................................................................................... 15

5.4. Custom Mode................................................................................................................... 17

6. Maintenance.................................................................................................................................... 18

6.1. Cleaning the pipette...................................................................................................... 18

6.2. Battery replacement...................................................................................................... 18

7. Troubleshooting.............................................................................................................................. 18

8. Warranty information..................................................................................................................20

9. Performance specifications .......................................................................................................20

10. Recycling instruction (WEEE) ....................................................................................................20

1. Intended use

Biohit eLINE Dispenser Pro is intended to be used in liquid

handling applications for dispensing liquids in volume range

of 0,1 µl to 50 ml. It is recommended to use Biohit Dispenser

Tips to ensure the best compatibility and performance with

eLINE Dispenser Pro.

This liquid handling device is designed and manufactured to

be used as general purpose laboratory equipment.

2. Biohit eLINE Dispenser Pro

The eLINE electronic pipettes offer you the latest technology

and innovations in the field of the hand held liquid handling

devices. The attractive and ergonomic design of the eLINE

pipettes together with electronic tip ejection operation take

the effort out of pipetting and reduce the risk of repetitive

strain injuries (RSI) that are common in mechanical pipetting.

The microprocessor controlled system and the superior

construction of the eLINE pipettes enables maximum liquid

handling performance with high levels of accuracy and

precision.

eLINE Dispenser

Cat. No. 73070X

X: 0=Without adaptor; 1=With AC-adaptor (EU, UK, US/JPN, AUS; KOR

and CHN plugs)

eLINE Dispenser Tips

Standard

Tip size (ml) Qty/Unit Order code

0,1 100 792017

0,2 100 792018

0,5 100 792019

1,0 100 792020

2,5 100 792021

5,0 100 792022

10,0 100 792023

25,0 25 792024

50,0 25 792025

Dispenser Test Package

0,5 20 792038

1,0 20

2,5 20

5,0 20

10,0 20

Sterile

Tip size (ml) Qty/Unit Order code

0,1 100 792026

0,2 100 792027

0,5 100 792028

1,0 100 792029

2,5 100 792030

5,0 100 792031

10,0 100 792032

25,0 25 792033

50,0 25 792034

Adapter

Adapter for 25 and 50 ml tips

Adapter 3792036

NOTE: The tips are disposable, do not reuse them!

3. Quick start

3.1. Unpacking

The Biohit eLINE Dispenser may be delivered with or without

an AC-adaptor, depending on the type of order. However, all

eLINE Dispenser packages contain:

- eLINE Dispenser

- Tilting piece

- Instruction manual

- Performance certificate in accordance with ISO 8655-5

- Demo package of the Sartorius Biohit eLINE Dispenser Tips

- 0.1 ml, 1 pc

- 0.2 ml, 1 pc

- 0.5 ml, 5 pcs

- 1.0 ml, 5 pcs

- 2.5 ml, 5 pcs

- 10.0 ml, 5 pcs

- 25.0 ml, 1 pc

- Adapter for 25 ml tip, 1 pc

Please check that all items are included and no damage has

occurred during shipment.

3.2. Charging the Biohit eLINE

Dispenser Pro before first use

The battery is protected against discharging during storage

with a plastic protector. Before use remove the plastic

protector.

1. Push the charging buttons simultaneuously in order to

open the battery cover

2. Remove the plastic protector

NOTE: It is recommended to charge the pipette for 12

hours before initial use. The pipette may only be charged

with original Charging Stand, Charging Carousel or eLINE

AC-adaptor (See Section 3.)

1. 1.

3.3. Biohit eLINE Dispenser Pro operating

instructions

The control and programming of the eLINE Dispenser is

performed using the display, keyboard, operating buttons

and tip.

Keyboard

Display

Start

button

Tip

ejection

buttons

Tip

connector

eLINE

Dispenser

Tip

Charging

contacts

Battery

cover

Reset

button

3.3.1. Display

NOTE: When the dispenser is not connected to the charging

unit the dispenser/display will automatically hibernate after

10 minutes. The dispenser can be switched on by pressing

any key.

Main Modes and Mode Symbols:

Custom mode (CST):

Main Modes/Operating

Mode symbols

Aspirating/Dispensing

direction

Battery

symbol

Speed

in/out

Aliquots/

counter

Volume

Selected tip

d=dispensing

d=(with TipGuide)

dST= dispensing

(user selects the

tip size)

P = pipetting

dd = diluting

Sd = sequential

dispensing

Ad = automatic

multidispensing

SA = multi-aspirating

GL Sr SET

3.3.2. Keyboard

3.3.3. Reset Button

To reset the dispenser, press the reset button on the battery

cover. The display shows E and TIP in blinking form and

pressing the tip ejector button will reset the pipette to the

d-mode.

NOTE: The dispenser should never be reset during pipetting!

Resetting will cause the plunger to move to the home position

and it will empty the contents of the tip. The tip is also ejected

in the end.

Select

Start button

Arrow up/down

Tip ejection buttons

Enter

Reset button

- Press to go back to the Main

Modes or to the previous step.

- Activates the speed-function

during the programming

procedure.

- Press to select the

operating mode or to

make setting changes

(volume, aliquots, speed,

etc.)

- Press to confirm the

selections or setting

changes

- Triggers all piston

movements

- Press to remove tip - Press to reset the pipette

Operating buttons:

3.3.4. Start Button

The start button triggers the aspiration and dispensing

operations according to the selected operating mode. Only a

soft touch is required to operate the button.

3.3.5. Tip Attachment and Ejection

Tip attachment

1. Push the tip into the tip connector. Make sure that the tip

is properly attached.

2. Confirm the tip size by double clicking the Start-button

according the instruction on the page 15.

Releasing the tip:

1. Press the Tip Ejection button. The tip is released

immediately.

NOTE: After the dispensing cycle has been completed a small

residual amount of fluid may have been left in the tip. During

the tip ejection it is possible that a part of the remaining fluid

comes out of the tip. Therefore it is recommended that the tip

ejection is performed above a waste container.

NOTE: When aspirating the liquid using the 25 or 50 ml tip,

let the tip stay in the liquid a few seconds after the plunger

has stopped moving. This way you will ensure that all liquid

has been aspirated into the tip.

NOTE: It is possible to work with aqueous liquids and liquids

with higher viscosity. However, it is recommended always

to choose the tip with the smallest applicable volume in

conjunction with high viscosity liquids. Please note, the

specifications are given in accordance with ISO8655-5.

4. Charging of the Biohit eLINE

Dispenser Pro

The eLINE Dispenser should be charged using the original

eLINE Charging Stand, Charging Carousel or AC-adapter.

When the dispenser is properly connected to the charger, the

led indicator lights up and stays on as long as the dispenser is

connected to the charger.

When the dispenser requires charging, the battery symbol and

“lo bat”-text will appear in the display. You need to confirm

Led indicator

Battery symbol

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