Bio-Tek ELx50 Manual de usuario

Operator’s Manual

All Models

Microplate Strip Washer

ELx50™

Microplate Strip Washer

Operator’s Manual

August 2008

Part Number 4071000

Revision M

BioTek®Instruments, Inc.

ii | Preface

BioTek Instruments, Inc.

Notices

BioTek®Instruments, Inc.

Highland Park, P.O. Box 998

Winooski, Vermont 05404-0998 USA

reproduced, transcribed, or transmitted in any form, or by any means electronic or

mechanical, including photocopying and recording, for any purpose other than the

purchaser’s use without written permission of BioTek Instruments, Inc.

Trademarks

BioTek®is a registered trademark, and ELx50™ and Liquid Level Alert™ are

trademarks of BioTek Instruments, Inc. Microsoft®and Excel®are either registered

trademarks or trademarks of Microsoft Corporation in the United States and/or

other countries. All other trademarks are the property of their respective holders.

Restrictions and Liabilities

Information in this document is subject to change and does not represent a

commitment by BioTek Instruments, Inc. Changes made to the information in this

document will be incorporated in new editions of the publication. No

responsibility is assumed by BioTek for the use or reliability of software or

equipment that is not supplied by BioTek or its affiliated dealers.

Contents | iii

ELx50 Operator's Manual

Contents

Notices ................................................................................. ii

Contact Information .................................................................. viii

Document Conventions............................................................... ix

Revision History.......................................................................... x

Intended Use Statement ............................................................ xiii

Quality Control ......................................................................... xiii

Repackaging and Shipping ......................................................... xiii

Warnings ................................................................................. xiii

Hazards and Precautions............................................................xiv

CE Mark Information .................................................................xvi

Electromagnetic Interference and Susceptibility ........................... xvii

User Safety............................................................................. xvii

Safety Symbols ...................................................................... xviii

Chapter 1: Introduction.................................................................1

Introducing the ELx50 Microplate Strip Washer ............................... 2

ELx50 Washer Variations ............................................................. 3

Manifold Variations................................................................. 3

Carrier Variations ................................................................... 5

Software Variations ................................................................ 5

Hardware Features...................................................................... 7

Accessories Included ................................................................... 8

Optional Accessories ................................................................... 9

Specifications ........................................................................... 10

Microplates ......................................................................... 10

Electrical............................................................................. 10

Physical .............................................................................. 10

Environmental ..................................................................... 10

Hardware............................................................................ 10

Performance Specifications .................................................... 11

Product Support & Service ......................................................... 12

Contacting the Technical Assistance Center.............................. 12

Returning Instruments for Service/Repair ................................ 12

Chapter 2: Installation.................................................................13

Unpacking and Inspecting the Instrument .................................... 14

Setting Up the ELx50 ................................................................ 20

Operating Environment ......................................................... 20

Installing the Carrier and Manifold .......................................... 20

Microplate Carrier............................................................ 20

8-, 8s-, or 12-Channel Manifold ......................................... 21

16-Channel Manifold ........................................................ 22

iv | Preface

BioTek Instruments, Inc.

Aspiration Carrier (ELx50/8FMW)....................................... 23

Connecting the Tubes and Bottles........................................... 25

Before Connecting the Tubes and Bottles ............................ 25

Waste System................................................................. 28

Supply System................................................................ 31

Liquid Level Alert Option................................................... 34

Final Check..................................................................... 35

Attaching the Mist Shield....................................................... 35

Connecting the Power Supply and Cord ................................... 36

Serial Port for Communication With Other Devices .................... 36

Verifying Performance ............................................................... 37

Repackaging and Shipping ......................................................... 38

Before Repackaging the Instrument ........................................ 38

Repackaging the ELx50 and Its Accessories ............................. 38

Chapter 3: Operation ...................................................................51

ELx50 Front Panel..................................................................... 52

Keypad Description ................................................................... 53

Entering Program Names ........................................................... 54

System Startup ........................................................................ 54

Main Menu ............................................................................... 55

General Usage Guidelines .......................................................... 56

Before Running Any Program ................................................. 56

Maintenance........................................................................ 58

Programming Considerations ................................................. 58

Liquid Level Alert Option ................................................... 59

Wash ...................................................................................... 60

Run Wash Program............................................................... 60

Run Wash Program Options .............................................. 60

Define Wash Program ........................................................... 61

Define Wash Program Options ........................................... 61

Define Wash METHOD Program Options .............................. 62

Wash METHOD Defaults and Ranges................................... 63

Define Wash DISPENSE (DISP) Program Options.................. 64

Wash DISPENSE Defaults and Ranges ................................ 66

Define Wash ASPIRATION (ASPIR) Program Options............. 67

Wash ASPIRATE Defaults and Ranges................................. 69

Edit Wash Program............................................................... 71

Copy Wash Program ............................................................. 71

Delete Wash Program ........................................................... 71

Prime...................................................................................... 72

Define Prime Program........................................................... 72

Define Prime Program Options........................................... 72

PRIME Program Defaults and Ranges.................................. 73

Contents | v

ELx50 Operator's Manual

Run Prime Program .............................................................. 74

Edit Prime Program .............................................................. 74

Copy Prime Program............................................................. 75

Delete Prime Program........................................................... 75

Dispense ................................................................................. 76

Define Dispense Program ...................................................... 76

Run Dispense Program.......................................................... 76

Edit Dispense Program.......................................................... 77

Copy Dispense Program ........................................................ 77

Delete Dispense Program ...................................................... 78

Aspiration ................................................................................ 79

Define Aspiration Program ..................................................... 79

Define Aspiration Program Options..................................... 79

Define Aspirate-only Program Options ................................ 80

Aspirate-only Program Defaults and Ranges ........................ 80

Run Aspiration Program ........................................................ 80

Edit Aspiration Program ........................................................ 81

Copy Aspiration Program....................................................... 81

Delete Aspiration Program..................................................... 82

Soak ....................................................................................... 83

Define Soak Program............................................................ 83

Edit Soak Program ............................................................... 84

Copy Soak Program.............................................................. 84

Delete Soak Program............................................................ 85

Linking Programs ...................................................................... 86

Define Link Program ............................................................. 86

View Link Program ............................................................... 87

Run Link Program ................................................................ 87

Delete Link Program ............................................................. 88

Utility...................................................................................... 89

Tests.................................................................................. 89

Self-Test (SLFCHK).......................................................... 89

Configuration Information (CHKSUM) ................................. 89

Setup ................................................................................. 90

Manifold (MANIFLD)......................................................... 90

SENSOR......................................................................... 90

AutoPrime........................................................................... 91

ELx50™ Microplate Strip Washer Menu Maps ................................ 92

Chapter 4: Maintenance and Performance Verification

(IQ/OQ/PQ) ................................................................................97

Overview ................................................................................. 98

Recommended Maintenance Schedule.......................................... 99

Recommended Performance Verification Schedule ........................100

vi | Preface

BioTek Instruments, Inc.

Warnings & Precautions ............................................................101

Maintenance Programs .............................................................102

Prime Programs.......................................................................103

Daily Maintenance....................................................................104

DAY_RINSE ........................................................................104

AUTOPRIME........................................................................104

Overnight Maintenance .............................................................106

OVERNIGHT_LOOP ..............................................................106

RINSE_AND_SOAK ..............................................................107

Monthly Maintenance................................................................108

Tools and Supplies ..............................................................108

Cleaning the Bottles ............................................................109

Cleaning the Manifold ..........................................................109

Cleaning the Aspirate and Dispense Tubes ..............................110

8-, 8s-, or 12-Channel Manifold ........................................110

16-Channel Manifold .......................................................110

Cleaning the Carrier ............................................................111

Microplate Carrier...........................................................111

Aspiration Carrier (ELx50/8FMW only) ...............................112

Cleaning the Check Valves....................................................114

Long-Term Storage Maintenance................................................115

LONG_SHUTDOWN..............................................................115

Disinfect the Aspiration Carrier (ELx50/8FMW) ........................116

Storing the ELx50™ .................................................................116

Decontamination .....................................................................117

Purpose .............................................................................117

Tools and Supplies ..............................................................118

Procedure for External Components .......................................119

Procedure for Tubing and Manifold.........................................119

Alternate Decontamination Procedure for Tubing and Manifold ...120

Procedure for Aspiration Carrier (ELx50/8FMW) .......................120

Performance Verification ...........................................................122

Installation Qualification (IQ).....................................................123

Operational Qualification (OQ) ...................................................123

Performance Qualification (PQ) ..................................................124

Self-Test and Checksum Test.....................................................125

Self-Test............................................................................125

Configuration Information (CHKSUM) .....................................125

Liquid Tests for the 8-, 8s- and 12-Channel Manifolds ...................126

Evacuation Efficiency and Dispense Precision Tests ..................126

Materials .......................................................................126

Evacuation Efficiency Test Procedure .................................127

Dispense Precision Test Procedure ....................................128

Liquid Tests Worksheet (8-, 8s-, and 12-Channel Manifolds)......129

Contents | vii

ELx50 Operator's Manual

Liquid Tests for the 16-Channel Manifold .....................................130

Evacuation Efficiency and Dispense Precision Tests ..................130

Materials .......................................................................130

Evacuation Efficiency Test Procedure .................................131

Dispense Precision Test Procedure ....................................132

Liquid Tests Worksheet (16-Channel Manifold) ........................133

Liquid Test for the Aspiration Carrier (ELx50/8FMW) .....................134

Evacuation Efficiency Test ....................................................134

Materials .......................................................................134

Evacuation Efficiency Test Procedure .................................134

Liquid Test Worksheet (Aspiration Carrier) ..............................136

Maintenance/Performance and Operational Logs...........................137

Chapter 5: Troubleshooting .......................................................141

Overview ................................................................................142

Troubleshooting Checklist.....................................................142

Troubleshooting Charts.............................................................143

Washer Start-Up .................................................................143

Fluid Aspiration...................................................................144

Fluid Delivery .....................................................................148

Fluid Leakage .....................................................................150

Carrier Movement ...............................................................151

Microplate Carrier...........................................................151

Aspiration Carrier (ELx50/8FMW)......................................151

Washer Manifold Movement ..................................................151

Microplate Scratches............................................................152

Syringe Drive Movement ......................................................152

Appendix A: Error Codes............................................................153

Appendix B: ELx50 Manifold Variations......................................159

Appendix C: Chemical Compatibility ..........................................163

Appendix D: Default Programs...................................................165

viii | Preface

BioTek Instruments, Inc.

Contact Information

BioTek®Instruments, Inc.

Highland Park, P.O. Box 998

Winooski, Vermont 05404-0998 USA

Customer Service and Sales

Internet: www.biotek.com

Phone: 888-451-5171 (toll free in the U.S.)

802-655-4740 (outside the U.S.)

Fax: 802-655-7941

E-Mail: [email protected]

Service/TAC

Phone: 800-242-4685 (toll free in the U.S.)

802-655-4740 (outside the U.S.)

Fax: 802-654-0638

E-Mail: [email protected]

European Coordination Center/Authorized European

Representative

BioTek®Instruments GmbH

Kocherwaldstrasse 34

D-74177 Bad Friedrichshall

Germany

Internet: www.biotek.de

Phone: +49 (0) 7136 9680

Fax: +49 (0) 7136 968 111

E-Mail: [email protected]

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