BIO RAD Tetrad 2 Manual de usuario

DNA Engine Tetrad®2

Peltier Thermal Cycler

Operations Manual

Version 2.0

PTC-0240 DNA Engine Tetrad 2 Cycler

DNA Engine Tetrad®2

Peltier Thermal Cycler

PTC-0240 DNA Engine Tetrad 2 Cycler

Operations manual

Supports software version 4.0

in any form, either print or electronic, is prohibited without written permission of Bio-Rad

Laboratories, Inc.

Alpha, Chill-out, CleanBox, Concord, Disciple Desktop, DNA Engine,

DNA Engine Dyad, DNA Engine Tetrad, Dyad Disciple, Hard-Shell, Hot Bonnet,

iProof, iTaq, Microseal, Moto Alpha, Multiplate, Power Bonnet, Slide Chambers and

Tetrad are trademarks belonging to Bio-Rad Laboratories, Inc.

Teflon is a trademark of E.I. DuPont de Nemours and Company. Windows is a trademark

of Microsoft Corporation.

NOTICE TO PURCHASER

This base unit, Serial No. ____________, in combination with its immediately attached

Bio-Rad sample block module(s), constitutes a thermal cycler whose purchase con-

veys a limited non-transferable immunity from suit for the purchaser’s own internal

research and development and for use in applied fields other than Human In Vitro

Diagnostics under one or more of U.S. Patents Nos. 5,656,493, 5,333,675, 5,475,610

(claims 1, 44, 158, 160-163 and 167 only), and 6,703,236 (claims 1-7 only), or corre-

sponding claims in their non-U.S. counterparts, owned by Applera Corporation. No

right is conveyed expressly, by implication or by estoppel under any other patent

claim, such as claims to apparatus, reagents, kits, or methods such as 5’ nuclease

methods. Further information on purchasing licenses may be obtained by contacting

the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City,

California 94404, USA.

05184 rev E

iii

Table of Contents

Explanation of Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .iv

Safety Warnings and Guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .iv

Electromagnetic Interference and FCC Warning . . . . . . . . . . . . . . . . . . . . . . . . . . . .v

Documentation Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .vi

Chapter 1: Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1

Chapter 2: Layout and Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Chapter 3: Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Chapter 4: Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Chapter 5: Creating Programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Chapter 6: Managing and Editing Programs . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Chapter 7: Running Programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1

Chapter 8: Using the Utilities . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1

Chapter 9: Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1

Chapter 10: Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-1

Appendix A: Warranties . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .A-1

Appendix B: End-User and License Agreement . . . . . . . . . . . . . . . . . . . . . . . . . .B-1

Appendix C: Factory-Installed Protocols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-1

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .In-1

Declaration of Conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .DoC-1

Explanation of Symbols

CAUTION: Risk of Danger! Wherever this symbol appears, always consult note

in this manual for further information before proceeding. This symbol identifies

components that pose a risk of personal injury or damage to the instrument if

improperly handled.

CAUTION: Risk of Electrical Shock! This symbol identifies components that

pose a risk of electrical shock if improperly handled.

CAUTION: Hot Surface! This symbol identifies components that pose a risk of

personal injury due to excessive heat if improperly handled.

Safety Warnings and Guidelines

Warning: When removing an Alpha unit from a DNA Engine Tetrad 2 base, keep

all fingers and foreign objects away from the Alpha unit bays. Keep all

objects clear of the Alpha unit bays until the fan has come to rest.

Warning: Operating the DNA Engine Tetrad 2 cycler before reading this manual

can constitute a personal injury hazard. Only qualified laboratory per-

sonnel trained in the safe use of electrical equipment should operate

these machines.

Warning: Do not open or attempt to repair the DNA Engine Tetrad 2 cycler, any

Alpha unit, or any accessory to the cycler. Doing so will void your war-

ranties and can put you at risk for electrical shock. Return the

DNA Engine Tetrad 2 cycler to the factory (US customers) or an author-

ized distributor (all other customers) if repairs are needed.

Warning: All Alpha unit blocks can become hot enough during the course of

normal operation to cause burns or cause liquids to boil explosively.

Wear safety goggles or other eye protection at all times during operation.

Warning: The DNA Engine Tetrad 2 cycler incorporates neutral fusing, which

means that live power may still be available inside the machine even

when a fuse has blown or been removed. Never open the

DNA Engine Tetrad 2 base; you could receive a serious electrical shock.

Opening the base will also void your warranty.

Caution: Never remove an Alpha unit from the DNA Engine Tetrad 2 cycler with

the power turned on and a program running. Doing so can cause elec-

trical arcing that can melt the contacts in the connector joining the

Alpha unit to the cycler.

Caution: Do not attempt to unpack or move the DNA Engine Tetrad 2 cycler

alone. Doing so could results in personal injury. Always enlist the help of

another individual when moving or lifting the DNA Engine Tetrad 2 cycler.

Safe Use Guidelines

The DNA Engine Tetrad 2 cycler is designed to be safe to operate under the following

conditions:

• Indoor use

• Altitude up to 2000 m

• Ambient temperature 5–31°C

• Relative humidity 10–90%, noncondensing

• Transient overvoltage per Installation Category II, IEC 664

• Pollution degree 2, in accordance with IEC 664

Electromagnetic Interference

This device complies with Part 15 of the FCC Rules. Operation is subject to the

following two conditions: (1) this device may not cause harmful interference, and (2)

this device must accept any interference received, including interference that may

cause undesired operation.

This device has been tested and found to comply with the EMC standards for emissions

and susceptibility established by the European Union at time of manufacture.

This digital apparatus does not exceed the Class A limits for radio noise emissions

from digital apparatus set out in the Radio Interference Regulations of the Canadian

Department of Communications.

LE PRESENT APPAREIL NUMERIQUE N'EMET PAS DE BRUITS RADIOELEC-

TRIQUES DEPASSANT LES LIMITES APPLICABLES AUX APPAREILS NUMERIQUES

DE CLASS A PRESCRITES DANS LE REGLEMENT SUR LE BROUILLAGE RADIO-

ELECTRIQUE EDICTE PAR LE MINISTERE DES COMMUNICATIONS DU CANADA.

FCC Warning

Warning: Changes or modifications to this unit not expressly approved by the party

responsible for compliance could void the user’s authority to operate the equipment.

Note: This equipment has been tested and found to comply with the limits for a Class

A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to

provide reasonable protection against harmful interference when the equipment is

operated in a commercial environment. This equipment generates, uses, and can

radiate radiofrequency energy and, if not installed and used in accordance with the

instruction manual, may cause harmful interference to radio communications.

Operation of this equipment in a residential area is likely to cause harmful interference

in which case the user will be required to correct the interference at his own expense.

Shielded cables must be used with this unit to ensure compliance with the Class A

FCC limits.

Note Regarding FCC Compliance: Although this design of instrument has been tested and found to comply with Part

15, Subpart B of the FCC Rules for a Class A digital device, please note that this compliance is voluntary, for the

instrument qualifies as an "Exempted device" under 47 CFR § 15.103(c), in regard to the cited FCC regulations in

effect at the time of manufacture.

Documentation Conventions

Before describing the various features of the DNA Engine Tetrad 2 cycler, let’s define

some “common ground” conventions.

• << >> will be used to indicate actual keys on the control panel, such as

<<ENTER>>, <<1>> and <<LEFT>>.

• < > will be used to indicate windowed menu items or buttons, such as <PRO-

GRAMS>, <RUN> and <UTILITIES>.

•Italics will be used to indicate windowed items that are not drop down menu

items or buttons, such as Calculated, Block, and Tracking. Typically, these will be

parameter selection items.

•Select is meant to be synonymous with click on, point-and-click, and any

phraseology implying selection of menu or option items with the mouse. In some

instances, selection is also possible by clicking the <<Enter>> button.

___________________________________________________________________________

This instrument system is labeled, “For Research Use Only.”

1-1

Introduction

Meet the DNA Engine Tetrad 2 Cycler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2

Using This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2

Important Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3

DNA Engine Tetrad 2 Thermal Cycler Operations Manual

1-2 Tech Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com

Meet the DNA Engine Tetrad®2 Cycler

Thank you for purchasing an DNA Engine Tetrad 2 cycler. Designed by a team of

molecular biologists and engineers, the DNA Engine Tetrad 2 cycler delivers multi-

block thermal cycling with superior thermal performance. The programmable

DNA Engine Tetrad 2 cycler with its 4-bay chassis is ideal for running multiple proto-

cols and accommodating multiple users.

The DNA Engine Tetrad 2 cycler offers the following features:

• Interchangeable sample blocks—the Alpha™unit family—accommodate a

variety of tubes, microplates, and slides

• Hot Bonnet®heated lid for oil-free cycling or the Moto Alpha™unit for auto-

mated systems

• Intuitive DNA Engine Tetrad 2 system software with user-friendly interface for

programming, editing, file management and much more

• Choice of calculated temperature control for highest speed and accuracy, or

of block or temperature control for compatibility with protocols designed for

a variety of instrument types

• Instant Incubate feature for continuous-temperature incubations

Using This Manual

This manual contains instructions for operating your DNA Engine Tetrad 2 cycler

safely and productively:

• Chapter 2 acquaints you with the physical characteristics of the

DNA Engine Tetrad 2 cycler.

• Chapters 3–4 present the basics of installation and operation for the

DNA Engine Tetrad 2 cycler.

• Chapters 5, 6 and 7 describe the creation, editing, and running of programs.

• Chapter 8 outlines the software utilities.

• Chapter 9 explains the proper maintenance of the DNA Engine Tetrad 2

cycler.

• Chapter 10 offers troubleshooting information for the DNA Engine Tetrad 2

cycler.

Introduction

Tech Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com 1-3

Important Safety Information

Safe operation of the DNA Engine Tetrad 2 cycler begins with a complete under-

standing of how the instrument works. Please read this entire manual before

attempting to operate the DNA Engine Tetrad cycler. Do not allow anyone who has

not read this manual to operate the instrument.

Warning: The DNA Engine Tetrad 2 cycler can generate enough heat to

inflict serious burns and can deliver strong electrical shocks if

not used according to the instructions in this manual. Please

read the safety warnings and guidelines at the beginning of

this manual on pages iv and v, and exercise all precautions

outlined in them.

Warning: Do not block the DNA Engine Tetrad 2 cycler’s air vents (see

figs. 2-1 and 2-4 for locations). Obstructing air vents can lead

to overheating and slightly enhanced risk of electrical shock

and fire.

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