BI 399 Manual de usuario

Air-Jacketed Large Capacity

Flo-Thru CO2Incubators

Model No.

399

399-1

399GWH

057-641-00 • 6/8/05

Safety Information ........................................................................................................................................................3

Alert Signals ..........................................................................................................................................................3

Specifications ..............................................................................................................................................................4

Electrical Requirements ........................................................................................................................................4

Temperature Range ..............................................................................................................................................4

Temperature Control..............................................................................................................................................4

Uniformity in Chamber ..........................................................................................................................................4

Gas Flow Rates ....................................................................................................................................................4

Volume ..................................................................................................................................................................4

Humidity ................................................................................................................................................................4

Dimensions ............................................................................................................................................................4

Shelves ..................................................................................................................................................................4

Net Weight ............................................................................................................................................................4

Options ..................................................................................................................................................................4

Unit’s Environmental Operating Conditions ..........................................................................................................5

Unpacking and Installation ..........................................................................................................................................6

Shipping Carton ....................................................................................................................................................6

Location ................................................................................................................................................................6

Electrical Power ....................................................................................................................................................6

Shelf Positioning ..................................................................................................................................................6

Adjustable Screw Guards for Protecting Back of Unit ..........................................................................................7

Hooking Up Water ................................................................................................................................................7

Connecting Condensate Drain to the Incubator ....................................................................................................7

Air/CO2 Hook-Ups ................................................................................................................................................8

CO2 Connection: Standard and Siphon Type CO2 Gas Cylinders ......................................................................9

Operation....................................................................................................................................................................10

Auto Tune ............................................................................................................................................................12

For Best Results ..................................................................................................................................................12

Auto-Tuning Procedure........................................................................................................................................12

Temperature Calibration ......................................................................................................................................13

Setting the Hi-Limit Thermostat ..........................................................................................................................14

Loading the Incubator..........................................................................................................................................14

Air/Gas Ratio ......................................................................................................................................................15

Humidity Adjustment............................................................................................................................................16

CO2 Sampling/Measurement ..............................................................................................................................16

Air and CO2 (or Nitrogen) ..................................................................................................................................16

Humidification ......................................................................................................................................................17

Humidifying without the Optional Humidifier ................................................................................................17

Humidifying with the Optional Humidifier......................................................................................................17

About the Kind of Water to Use ....................................................................................................................18

Demineralizer Cartridge................................................................................................................................19

Operation of Humidification System ............................................................................................................20

Periodic Cleaning and Inspecting of Humidifier............................................................................................20

Humidity Controller ......................................................................................................................................22

Auto Tune ....................................................................................................................................................23

Offset Humidity Calibration ..........................................................................................................................23

Operating Suggestions ................................................................................................................................24

Hints on Using Fyrite ..........................................................................................................................................25

Taking a Fyrite Reading...................................................................................................................................... 26

Kwik Inject Automatic CO2 Recovery System ....................................................................................................29

Built-In Compressed Air Supply ..........................................................................................................................29

Maintenance ..............................................................................................................................................................30

Replacing Temperature and/or Humidity Controller ............................................................................................30

CO2 and Air Bubbler............................................................................................................................................30

Adjusting Automatic CO2 Recovery System ......................................................................................................31

Replacement Parts ....................................................................................................................................................33

Ordering Procedures ..................................................................................................................................................34

Decontamination Statement ......................................................................................................................................35

Two Year Limited Warranty ........................................................................................................................................36

Table of Contents

The Barnstead|Lab-Line Large-Capacity, 34 cu. ft. (963

liter), Flo-Thru CO2 Incubator has been specially

designed for laboratories that handle a high volume

of work. The unit has been factory tested and needs only

to be placed in a suitable location and connected to the

required electrical supply and air and CO2 (or nitrogen)

sources at a pressure of 15 psi. A blower fan assists in

maintaining uniformity of the CO2 concentration in the

chamber, as well as, minimizing any temperature gradi-

ents.

All controls are contained in the control panel. The micro-

processor- based controller maintains temperature from

slightly above ambient to 60ºC. The hydraulic hi-limit

thermostat controls temperature a few degrees above the

user established set point in the event the main controller

fails. This provides an added measure of protection to

the chamber.

The KWIK-INJECT™ system provides quicker recovery

and a more stable chamber environment. CO2 is inject-

ed into the chamber to compensate for losses incurred

during door openings. A timer on the control governs

injection time from 3 to 60 seconds.

6 perforated stainless steel shelves provide a total area of

36 sq. ft. (3 sq. m) with a load capacity of 100 lbs. (45 kg)

per shelf when the load is evenly distributed, 65 lbs. (29

kg) per shelf when the load is concentrated.

Humidification may be obtained simply by placing a pan

of deionized or distilled water on one of the shelves. A

humidifier and controller are available as factory-installed

options and require a demineralized water source. The

humidifier is controlled from the front panel and allows

regulation of the relative humidity from approximately

20% above ambient to 98% RH. A panel meter gives a

direct reading of relative humidity.

The exterior of the incubator is made from cold rolled

steel with a durable powder coated finish. The interior

and door are made of stainless steel. Inside the door and

between the double walls is 3-inch thick glass wool insu-

lation that helps hold heat loss to a minimum and pro-

motes temperature uniformity in the chamber.

Model 399GWH has an optional humidifier with controller.

Safety Information

Warning

Warnings alert you to a possibility of

personal injury.

Caution

Cautions alert you to a possibility of

damage to the equipment.

Note

Notes alert you to pertinent facts and

conditions.

Hot Surface

Hot surfaces alert you to a possibility of

personal injury if you come in contact

with a surface during use or for a period

of time after use.

Alert Signals

Electrical Requirements

399, 399GWH: 120 Volts, 50/60 Hz, 1400 Watts, 11.7 Amps

399-1: 230/240 Volts, 50/60 Hz, 1400 Watts, 5.8 Amps

Temperature Range

Ambient +5°C to 60°C

Temperature Control

±0.5°C

Uniformity in Chamber

±0.5°C

Gas Flow Rates

Air: 1.0 to 14.0 liters/minute

CO2: 0.1 to 1.2 liters/minute

Volume

34 cu. ft. (963 liters)

Humidity

Range: ambient to 98% RH

Accuracy: ±3% RH

Dimensions

Exterior: 42”W x 35”D x 91”H (107cm x 89cm x 231cm)

Chamber: 36”W x 25”D x 65”H (91cm x 64cm x 165cm)

Specifications

Shelves

6 perforated, stainless steel

Net Weight

980 lbs. (445 kg)

Options

H: Humidity Control

L: Left Hand Door

P: Built-in compressed air supply

GW: Glass Window 12” x 48” (30 x 122 cm)

Unit’s Environmental Operating Conditions

Pollution Degree: 2

Installation Category: II

Altitude: 2000 meters MSL (Mean Sea Level)

Humidity: 80% maximum, non-condensing

Electrical Supply: 120VAC or 240VAC

Voltage Tolerance: ±10% of normal rated line

Temperature: 15°C to 40°C

Product Usage: This product is intended for use indoors only

SPECIFICATIONS

Shipping Carton

The shipping carton should be inspected upon delivery.

When received, carefully examine for any shipping dam-

age before unpacking. If damage is discovered, the deliv-

ering carrier should both specify and sign for the damage

on your copy of the delivery receipt.

Open the carton carefully making certain that all parts are

accounted for before packaging materials are discarded.

After unpacking, if damage is found promptly report it to

the carrier and request a damage inspection promptly.

IMPORTANT: Failure to request an inspection of damage

within a few days after receipt of shipment absolves the

carrier from any liability for damage. You must call for a

damage inspection promptly.

Location

Select a location for the incubator that is free of drafts

and extraneous vibrations. The unit should be level.

Electrical Power

Turn all controls to the off position. Connect the power

cord of the incubator to an outlet providing the power

characteristics specified on the unit's nameplate or in this

manual.

Shelf Positioning

The 6 perforated stainless steel shelves are adjustable on

1/2" (12 mm) centers. Place the shelf support clips

where desired and then set shelves in place. Shelves

and support clips can be ordered to provide additional

shelf space.

Unpacking and Installation

Warning

Do not use in the presence of flam-

mable or combustible materials or

explosive gases. Do not use in the

presence of pressurized or sealed

containers. Fire or explosion may

result, causing death or severe

injury.

Warning

Do not heat any substance above a

temperature that will cause it to emit

toxic fumes. Death or severe injury

may result.

Warning

Use only an inert gas such as car-

bon dioxide in the incubator. Do not

under any circumstance inject oxy-

gen or other explosive gas or mix-

ture into unit. Failure to observe

these precautions can result in

explosion and/or fire and serious

injury or death to personnel and

property damage.

Adjustable Screw Guards for

Protecting Back of Unit

Exercise care in placing the back of the unit against a wall

to avoid damage to CO2 tubing and related attachments.

To protect against this, 2 adjustable screw guards on the

back are used to establish a buffer zone for the CO2 tubing.

If unit is shipped upright, guards are in place; if shipped flat,

guards are furnished loose and must be inserted.

Hooking Up Water

Connect supply water line to the 1/4” NPT fitting located on

the inlet side of the D. I. cartridge. The recommended

water pressure setting on the regulator gauge should be

adjusted to read between 10 and 15 psi.

Connecting Condensate Drain to

the Incubator

1/2” drain lines are located in back of the incubator.

UNPACKING AND INSTALLATION

Turn all flow meters on the incubator fully clockwise (closed).

Connect air and CO2 supply lines to their respective fittings on

the back of the unit. Use 1/4" (6 mm) ID tubing, flexible metal

hose, etc., (meeting local codes), with filters to ensure that the

air and CO2 are free of contaminants, for gas lines.

Set the supply regulators at 15 psi. Check all of the connec-

tions for leaks.

UNPACKING AND INSTALLATION

Air/CO2 Hook-Ups

Turn the power switch to OFF and insert the plug into the outlet.

To Air Fitting

To CO2 Fitting

100% Air 100% CO2

1/4" Hose Barb

Connections

Set pressure at 15 psi.

Pressure regulators and gauges must be

installed on each tank. Check local gas suppli-

er for proper regulators.

If air pump is used instead of tank, air must be

filtered to remove water, oil and dirt.

Warning

High concentrations of carbon diox-

ide produce metabolic abnormalities,

disturbances of the central nervous

system and cardiac instability.

Unconsciousness may occur at con-

centrations above 10%.

Note

It is our recommendation that this

unit run 24 hours empty before intro-

ducing media to be incubated. This

will facilitate ease of servicing if

required as well as nullify the need

to decontaminate the chamber in the

event a problem occurs.

This unit cannot be accepted for

servicing or credit by Barnstead

International unless accompanied by

a completed Certification of

Decontamination form, included at

the end of this manual.

Standard Type (Correct) Siphon Type (Incorrect)

1. Compressed Gas Association #320

connector.

2. Gas head space.

3. Liquid CO2 filled to 68% equal weight

of water that cylinder would hold at

60ºF.

1. Warning tag indicating that cylinder is siphoning type.

2* Aluminum ring.

3* Gold band.

4* Stamp or marking on cylinder: "Siphon" or "Eductor

Tube".

5* Valve should be of special type for service.

6* Withdrawal tube draws up the liquid CO2.

7* Gas head-space.

8* Liquid CO2 filled to 68% equal weight of water that

cylinder will hold at 60ºF.

*Note: Some gas suppliers will have no markings to indicate

an “Educator Tube” or “Siphon” type cylinder. Be sure to order

DRY, LAB-GRADE CO2.

Be sure to obtain a dual-stage regulator from the gas supplier

for the CO2 tank that is to be installed according to local

codes. Connect 1/4" ID (6.35 mm) flexible tubing to the regu-

lator. Connect the other end of the tubing to the unit's CO2

inlet (located on the back top panel). Insert tubing over hose

barb and fasten with an appropriate clamp to assure a proper

connection. Adjust the regulator for the pressure recommend-

ed on the previous pages when CO2 is to be injected into

chamber. For optimum results, do not exceed or reduce this

pressure.

CO2 Connection: Standard and Siphon Type CO2 Gas Cylinders

UNPACKING AND INSTALLATION

Operation

Amicroprocessor-based automatic tuning controller with a

platinum RTD temperature sensor provides a display of

either chamber or set point temperatures. The micro-

processor used in the unit automatically tunes PID

parameters to fit the characteristics of individual thermal

systems.

Auto-tune is a user-initiated function. It provides a tune-at-

temperature function that means the controller runs the

auto-tune program when the incubator is at set point.

Program parameters are retained in the nonvolatile mem-

ory in the event that there is power failure or interruption.

1 2 3 4 5 6 7 8 9

1. Power Switch

2. Hi-Limit Thermostat

3. Hi-Limit Thermostat Status Lamp

4. Temperature Controller

5. Kwik-Inject Control Dial

6. Kwik-Inject Status Lamp

7. Sample Access Port

8. Air Flow Meter

9. CO2 Flow Meter

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