Avantik QS11 Manual de usuario
Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
1
Avantik
Cryostat
INSTRUCTION MANUAL
Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
2
CERTIFICATION
Avantik certifies that this instrument has been tested and checked carefully.
Its technical data was verified before shipment to be in accordance with the
published specifications.
The instrument complies with applicable international safety regulations.
WARRANTY
This Avantik product is warranted against defects in material and workmanship
for a period of 1 year. Parts which prove to be defective during the warranty
period will be repaired or replaced free of charge by the manufacturer. No other
other warranty is expressed or implied. Unauthorized modification or repair by third
party persons will void the warranty.
The warranty will expire in case of improper or wrong use of the instrument and in
case the warning and precautionary messages are not observed. Avantik is not
liable for any occurring damage.
Errors and omissions excepted. Subject to amendment and improvement without
further notice.
This instruction manual will be supplied together with each instrument. Further
copies can be ordered at the nearest Avantik sales office by giving the serial
number of the instrument, the number of the instruction manual and the date of
issue.
This instruction manual is available in the following languages:
Cat. No.
English: 387779
Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
3
INTENDED USE
Dear valued Customer,
Thank you for buying this Avantik instrument.
Before putting the instrument into operation, please read these operating
instructions carefully to familiarize you with its proper operation and
functions.
The Cryostat QS11 / QS11UV is a highly efficient cryostat for sectioning
techniques in routine and research.
Only skilled or specially trained personnel must operate the microtome
cryostat, i.e. placing the specimen onto a chuck, sectioning and transferring
sections onto a slide. The listed and marked safety measures as well as the
regulations and hygiene measures of your respective lab must strictly be
observed.
Serial No.: __________________
Please check the Serial No. on the type plate, which is placed on the rear
side of your instrument and enter this number here. This way, questions and
service can be handled faster.
Instruction Manual No. 387779
Issued on January 27th, 2011
Avantik
36 Commerce Street
07081 NJ Springfield
USA
Phone: +1.269.544.5600
Fax: +1.269.372.2485
Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
4
Intended Use
Table of Contents
Safety Precautions
PART 1 INTRODUCTION
1-1 Description of the QS11 / QS11UV
1-2 Technical data QS11 / QS11UV
PART 2 OPERATING INSTRUCTIONS
2-1 Setting up the cryostat
2-2 Initial turn-on
2-3 Basic operational rules
2-3-1 Actual and set value of the chamber temperature
2-3-2 Defrosting the evaporator
2-3-3 Interrupting a defrosting cycle
2-3-4 Emptying the defrosting liquid
2-3-5 Illumination of the cryo chamber
2-3-6 Freezing on a specimen
2-3-7 Heat extractors (optional accessories)
2-3-8 Specimen orientation with zero positioning
2-3-9 Handwheel lock
2-4 Cutting process
2-4-1 Cutting movement and retraction
2-4-2 Setting section thickness and trimming thickness
2-5 Feed
2-5-1 Specimen coarse feed
2-5-2 Memory function
2-5-3 Trimming and first cuts
2-5-4 Fine feed
2-6 Indication of cutting processes
2-6-1 Section counter
2-6-2 Sum of section thicknesses
2-6-3 Remaining travel to front end position
2-6-4 Setting the real time
2-7 Knife carriers
2-7-1 Standard knife carrier
2-7-2 Disposable blade carrier
2-7-3 Disposable blade carrier EC 70
2-8 Module U (Optional)
Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
5
2-9 Error code indication
2-9-1 Definition of the error codes
2-10 Accessories
2-10-1 Standard equipment
2-10-2 Additional equipment
PART 3 THEORY OF OPERATION
3-1 Microtome cooling, defrost cycle
3-2 Cutting movement
3-3 Specimen coarse feed and trimming stages
PART 4 WORKING WITH THE CRYOSTAT
4-1 Preparations on the microtome and inside the cooling chamber
4-2 Freezing on the specimen
4-3 Orientation and trimming of specimens
4-4 Temperature list for cryo sectioning
4-5 Sectioning and taking off sections
4-6 How to avoid malfunctions
4-6-1 Possible sources of errors – cause and removal
PART 5 MAINTENANCE AND CARE OF THE CRYOSTAT
5-1 Shutting-off for cleaning
5-2 Removing the microtome
5-3 Cleaning and care of the microtome
5-4 Cleaning the cooling lamella
PART 6 CONDITIONS FOR THE TRANSPORTATION OF THE INSTRUMENT
6-1 Taking back the instrument for repair or routine maintenance
6-2 Disposal of the instrument after final shutdown
Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
6
SAFETY PRECAUTIONS
WARNING SIGNALS AND SYMBOLS
The installation and routine use of the QS11 / QS11UV is easy and safe if the instructions in this manual are
being observed.
Note:
Special instructions regarding operation of the instrument.
Warning:
Special precautionary measures to prevent damage to equipment. For a long lifetime of the
equipment, please observe these instructions carefully.
Caution – general danger spot:
The instruction manual must strictly be observed whenever this symbol is visible on the
instrument.
Biohazard:
Warning of biological danger.
Radioactivity:
Warning of radioactive danger.
Separate taking back of electrical and electronic instruments in the countries of the
European Union:
This is to be applied in the countries of the European Union and other European countries with a
separate collecting system within the waste management.
This product, being an electro and/or electronic instrument, must be treated separately within the
waste management process (WEEE).
Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
7
SAFETY PRECAUTIONS
C A U T I O N !
The operator's safety is affected, when the instrument is not operated in accordance
with this instruction manual.
Please observe the following general precautions during operation of this
instrument. Failure to comply with these precautions violates safety standards and
the intended use of the instrument. Avantik is not liable for misuse of the
instruments and failure to comply with basic safety requirements.
INSTRUMENT GROUNDING
To avoid injury from electrical current, the instrument must be connected with the protective earth. The
instrument is equipped with a three wire ground plug. The power outlet must be connected to the protective
earth and must meet the International Electrotechnical Commission (IEC) regulations.
CAUTION: MAINS VOLTAGE
Never remove instrument covers during operation. Component replacements as well as adjustments must
only be made by trained service personnel. Unplug the unit before removing or opening the covers.
DANGER IN EXPLOSIVE ENVIRONMENT
The instrument must not be operated in the presence of flammable gases.
HAZARD OF FROSTBITE
Avoid permanent touching of metal parts inside the cryostat microtome chamber as frostbite may occur at
unprotected hands and arms.
HAZARD OF RADIOACTIVE RADIATION
When working with radioactive specimens observe all applicable radiation safety procedures. When working
with radioactive contaminated material, appropriate safety and disinfection measures must be carried out.
According to the rules and regulations concerning the handling of radioactive contaminated material of the
respective laboratory, safety clothing (e.g. particle mask, gloves, protective shoe covers) must be worn.
Radioactive contaminated waste must be disposed of according to the respective regulations.
Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
8
HAZARD OF INFECTION
Use the appropriate safety and disinfection measures when working with infectious specimens.
According to the rules and regulations concerning the handling of infectious/radioactive contaminated
material of the respective laboratory, safety clothing (e.g. particle mask, gloves, protective shoe covers) must
be worn.
HAZARD OF BIOLOGICAL DANGER
Specimens used during the intended operation of the instrument might potentially be infectious. For this
reason, it is recommended to observe the general laboratory regulations concerning protection against
danger of infection.
Information on decontamination media, their use, dilution and effective range of application can be read in
the Laboratory Biosafety Manual : 1984 of the World Health Organization.
HAZARD OF MALFUNCTION
To avoid the hazard of malfunction of an instrument, it must only be operated in a controlled electromagnetic
environment. This means, that transmitters such as mobile phones must not be operated in their close
vicinity.
In case of malfunctions and/or service work, please turn off the instrument and contact your local dealer.
CARE IN USING MICROTOME KNIFE
To diminish the danger of being injured by the knife or blade, use the knife guard when adjusting specimen
and knife. If possible, the specimen should be clamped in before the knife is inserted into the knife holder.
Before changing the knife holder, always remove blade or knife! Unused knives should always be kept in a
knife case. Never place the knife with the cutting edge upwards. Never try to catch a dropping knife!! Never
check the sharpness of the cutting edge with your fingers. The cutting edge is extremely sharp!
WASTE DISPOSAL
All debris, waste, defrosting liquid as well as infectious and radioactive contaminated material from operation
must be disposed of in accordance with the respective regulations of the lab. Disinfection and cleaning
liquids as well as section waste must be disposed of according to the respective regulations for special
waste!
Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
9
Fig. 1
Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
10
1 INTRODUCTION
1-1 DESCRIPTION OF THE QS11 / QS11UV
Open top cryostat. Stainless steel cooling
chamber. Chamber temperature regulation
variable from +5°C down to –35°C.
Graphic LED-display of set and actual
temperatures, electronic control with user-oriented
touchpad keyboard, battery-buffered memory and
self-diagnostic system.
Automatic defrosting and in addition a manual
defrost cycle which can be activated when
needed. Defrost interrupt on keystroke request.
Integrated fast freezing device down to max.
–60°C via Peltier element.
Sliding window with heater. Integrated fluorescent
lamp for cryo-chamber. Brush shelf and storage
space inside the cryo chamber. Large, flat storage
space on top of the housing with integrated
storage space for marked slides.
Rotary Microtome with backlash and
maintenance-free cross roller bearings in stainless
steel. Electro-mechanical feed system.
Section thickness setting from 1 to 20 microns;
up to 10 µm in 1 µm-increments,
up to 20 µm in 2 µm-increments,
Trimming thickness setting from 10 to 500
microns;
up to 80 µm in 10 µm-increments,
up to 100 µm in 20 µm-increments,
up to 300 µm in 50 µm increments and
up to 500 µm in 100 µm-increments.
Specimen retraction during the return stroke with
optical indication. Horizontal feed range 28 mm.
Vertical cutting stroke 60 mm.
Max. specimen size 70 x 55 mm.
Motorized coarse feed in two directions.
Limit indication and automated switch off at front
and rear limits of horizontal travel.
Section counter and indication of sum of section
thicknesses with reset-button. Indication of
remaining travel.
Mechanical handwheel brake in the lower reversal
point of the specimen orientation.
Fine orientation of specimen 8° universally in x/y-
axis with zero device. Rotatable on Z-axis, 360°.
Basic outfit with 3 x 30 mm chucks, 1 x 40 mm
chuck, 118 ml freezing medium, 100 ml cryostat
oil, brush shelf and section waste tray, consisting
of two parts.
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