Arrayit NanoPrint LM60 Manual de usuario
!
!
!
!
NanoPrint™ Enterprise Level LM60 and LM210
Microarray Printers
Users Manual
v.130710.1
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
2!
!
!
!
!
!
!
!
!
!
!
!
!
!
!
!
!
!
!
!
!
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
3!
Table of Contents
Introduction – page 4
System Overview – page 5
Software Features – page 7
System Options and Advantages – page 8
System Specifications and Features – page 9
System Installation and Performance – page 14
Ordering Information – page 22
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
4!
Introduction
NanoPrint™ microarrayer printers are enterprise level linear drive systems that provide
universal platforms for research and diagnostic microarray manufacturing. Based on
advanced nanotechnology, Arrayit NanoPrint™ robots move with 500 nanometer resolution
and deliver sub-nanoliter sample volumes using high throughput, patented contact printing
devices. Arrayit printing devices are covered by U.S. Patent 6,101,946 and these devices
require a license for diagnostic applications. Please contact us for licensing information
regarding diagnostic microarray manufacturing.
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
5!
System Overview
Figure 1. Shown is the NanoPrint™ LM60 system with a capacity of three 384-well sample
microplates printing onto 60 standard glass slide substrates. Worktables for printing into
microplates, plate size glass and other micro-fluidic biochip formats are available.
Figure 2. Shown is the NanoPrint™ LM210 system with a capacity of three 384-well sample
microplates printing onto 210 standard glass slide substrates.
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
6!
NanoPrint™ Microarrayers are industrial-level platforms for all
microarray manufacturing applications including DNA microarrays,
protein microarrays, and other types of biomolecules. NanoPrint™
systems enable the manufacture of microarrays utilizing ArrayIt®
Professional, 946 and Stealth Style Micro Spotting Pins. The
NanoPrint™ uses superior linear drive motion control technology
and proprietary Warp1 controllers, and is compatible with all
standard microarray surfaces made by ArrayIt® and other glass
substrate slide open platform vendors. Systems are easily configured to print onto different
types of surfaces including 96 and 384-well plates, plate-sized glass, and proprietary
cartridges and cassettes by taking advantage of flexible and modular deck configurations
and an easy to use software interface. The Microarray Manager Software combines
unparalleled power and simplicity in a graphical user interface. Click here.
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
7!
Software Features
•A Method Creation Wizard
•User & version control management
•Custom calibration of the slide and microplate positions
•Complete sample tracking
•Support of input and output data files
•Custom microarray designs
•Customizable speed profiles and wash protocols
•Automatic Method Validation
•Runtime sample and spotting views as well as a Simulation Mode
•Easy to use graphical Re-Print Wizard
The high speed, high precision linear servo control system of the NanoPrint™ produces
superior instrument performance that is essentially free of friction, noise and thermal
emission. Combined with the compact bench top design, user configurable worktable,
humidity and particulate control, a host of available options, and the flexible and
sophisticated software, NanoPrint™ systems offer the complete solution to high performance
microarray printing.
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
8!
System Options and Advantages
•192 Pin Printhead (48 Pin Professional Printhead provided as standard)
•Anti-vibration table
•Peltier-driven microplate cooling
•Microplate printing worktables
•Custom worktables for proprietary printing cassettes, biochips and cartridges
•Worktable conveyor
•Microplate stackers, refrigerated with lid removal and barcode reader
•Dehumidification
•Extended service warranty
The value of the linear servo motor technology used on NanoPrint™ Microarrayers:
•Nanometer scale positional accuracy
•Quiet operation
•No vibration
•No heat generation
•No motor dust
•Superior Accuracy
•Near maintenance-free operation
•High speed
•High load capacity
•Zero "sagging" with loss of power
•Safer and much more durable than stepper motors
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
9!
System Specifications and Features
Software
Interface
Intuitive and Graphic Windows 2000 or Windows XP User
Interface
User Management
Password protected access levels for User, Manager and
Administrator
Data Files
Both import and export of data files compatible with all
current microarray detection instruments
Version Control
Complete tracking of all methods, version and backups
Automatic Method Validation
Automatically validates for compatible method
parameters
Sample and printing Views
Each available in real-time during operation
Method Creation Wizard
Only minimal information required to create microarray
printing protocols
Simulation Mode
Pre-test print methods in software prior to running
programs
Flexible
Microarray design, pin configuration, worktable layout,
source and destination type, offsets, speed profiles,
wash/dry parameters and pre-print locations
Hardware
Size (L x W x H, cm)
LM60 (110 x 85 x 56 cm), LM210 (164 x 85 x 56 cm)
Weight (kilograms)
LM60 (150 kg), LM210 (200 kg)
Positional resolution
500 nanometers using linear drive Warp1 control
Printing speed
48 spots per second or 192 Spots second using “192”
Series Pins and Printheads.
Destination
Microarray substrates, glass slides, microplates, custom
Printing technology
Arrayit Pro, 946 or Stealth pins and printheads
Number of pins
Configurable 1 to 48 at 4.5 mm centers or 1 to 192 at
2.25mm
Spot diameter
65 microns or larger to meet all applications
Minimum spot spacing
50 microns
Pre-printing
User definable
Wash/Dry station
Ultrasonic with 2 wash positions and a dry station
Number of microplates
Three standard 384-well, customizable
Number of substrates
60 substrate slides (LM60) or 210 substrate slides
(LM210)
Number of microplates
15 96-well microplates (LM60) or 45 96-well microplates
(LM210)
Microplate type
96-well or 384-well microplates
Microplate cooling
Cool 1-3 microplates, for protein microarray applications,
or use the refrigerated stacker for higher throughput and
hands free automation.
Environmental control
Fully enclosed, HEPA filtration and user-defined humidity
control
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
10!
NanoPrint™ Flexible Deck
Configurations: Front left
view of the NanoPrint™
showing three microplates,
the wash/dry module and the
printhead and pins printing
onto glass substrates. A 48-
pin NanoPrint printhead
loaded with 48 946 Micro
Spotting Pins is printing
location 8 on the deck. The
modular deck design and
platen cassettes allow users to
easily switch between glass
substrates, microplates,
wafers and other types of
substrates.
Accessory Control Module
(ACM): The ACM unit provides
computer control for the
wash/dry, humidity, and
ultrasonication stations on the
deck of the NanoPrint™. The
ACM unit fits neatly beneath
the laboratory bench or under
the optional anti-vibration
table available with the
system. The ACM is extremely
quiet and operates only as
needed by the system.
Humidity is maintained in a
user-specified manner of
±1%. Wash buffers are stored
in carboys in front of the ACM.
NanoPrint™ Pin Cleaning
Module: NanoPrint™
microarrayers are each
equipped with a station that
provides pin washing, drying
and sonication of 1-48 pins.
The sonicator is automatically
filled and emptied during the
print run in a completely
automated manner. Systems
sensors prevent splashing and
overflowing for pin and deck
safety. Drying is accomplished
by vacuum using a quiet but
powerful ACM-controlled
function. The Pin Cleaning
Module is rugged, durable and
Este manual sirve para los siguientes modelos
1
Tabla de contenidos
