Applied Biosystems QuantStudio 3 Instrucciones de funcionamiento

QuantStudio™3 and 5 Real-Time PCR

Systems

Publication Number MAN0010405 Revision C.0

■Site preparation overview .............................................. 2

■Site preparation checklist ............................................... 3

■Customer responsibilities ............................................... 4

■Site requirements ...................................................... 5

■Instrument-to-computer connections .................................... 10

■Safety requirements .................................................. 12

■Receive and inspect the shipment ....................................... 13

■Move the instrument packages to the installation site ..................... 13

■Materials for installation and operation ................................. 14

■Download and install the desktop software .............................. 15

■Register to access the Thermo Fisher Cloud .............................. 16

■Documentation and support ........................................... 17

This guide contains the information needed to prepare your site for installation of the

QuantStudio™ 3 or 5 Real-Time PCR System.

SITE PREPARATION GUIDE

For Research Use Only. Not for use in diagnostic procedures.

Site preparation overview

Customers have the option to install the instrument themselves or purchase

installation service from the eld services team.

Note: Optional installation by a Thermo Fisher Scientic service representative

includes some basic operator training and a review of data during installation. If you

opt for a service representative to install the instrument, contact Thermo Fisher

Scientic to schedule the installation.

IMPORTANT! Thermo Fisher Scientic does not install, service, or repair instruments

in areas designated BioSafety Level 3 (BSL-3) or BioSafety Level 4 (BSL-4).

Before installing the instrument:

1. Review this guide.

2. Receive and inspect the shipment (page 13).

3. Ensure that the purchase order is complete.

4. Move the packaged instrument to the installation site (page 13).

5. Complete the site preparation checklist (page 3).

This task is required for a service representative to complete the installation.

After the instrument is unpacked, installation and verication take approximately

1 hour. For additional information, see the user documents that are provided with the

instrument.

Installation time

Site preparation overview

QuantStudio

™

3 and 5 Real-Time PCR Systems Site Preparation Guide | MAN0010405 C.0

Site preparation checklist

Complete all items in the following checklist before you install the instrument.

IMPORTANT! For installation by a Thermo Fisher Scientic service representative,

complete, date, and initial the following checklist before the scheduled installation

date. If the site preparation checklist is not complete when the service representative

arrives, the scheduled installation may be postponed.

3Date Initials Site preparation requirement See page

Customer responsibilities have been reviewed.

Personnel have been assigned tasks and responsibilities.

The installation site is identiﬁed and meets the following requirements:

Space and clearance 5

Environmental 7

Electrical 8

Instrument-to-computer connections 10

Safety 12

The instrument was received and inspected as follows: 13

All shipping list items are the items ordered at the time of purchase.

Any damage to shipping containers was reported to the shipping

company that delivered the instrument.

Any damage or mishandling was recorded on the shipping documents.

All reagents and plates are unpacked and stored as speciﬁed.

The installation site is cleared and ready for the instrument installation.

The packaged instrument and other shipping containers are moved to the

installation site.

All materials for installation and operation are available. 14

10 GB of Thermo Fisher Cloud storage. 16

The

QuantStudio

™

3 and 5 Real-Time PCR Systems IT Checklist

(Pub. No. MAN0013738) has been completed and returned. —

Site preparation checklist

QuantStudio

™

3 and 5 Real-Time PCR Systems Site Preparation Guide | MAN0010405 C.0

Customer responsibilities

Personnel Responsibilities

Site preparation/

installation

coordinator

• Receives and inspects the instrument.

• Unpacks and stores the reagents and plates as speciﬁed.

• Reviews the site preparation guide for safety information and the instrument requirements.

• Coordinates personnel and tasks.

• Chooses the installation site.

• Reviews checklists with applicable personnel, then the service representative[1], to verify

that the site is properly prepared.

• Schedules the installation and informs personnel of the installation day.

• Ensures that the site is clear of unnecessary material on the installation day.

• Is available during installation to assist the service representative.[1]

Laboratory safety

representative

• Reviews the site preparation guide for safety information.

• Ensures that the required safety practices and equipment are in place.

• Is available at all times while the service representative is at the customer's facility.[1]

Laboratory

personnel/

primary users

• Reviews safety information.

• Ensures that all customer-provided materials for installation are present at the site.

• Ensures that primary users (responsible for training other users) are available during

installation, so that they can be trained on the instrument.[1]

Facilities

personnel

• Ensures that the installation requirements are met for:

– Space at the installation site

– Building clearances

– Humidity and temperature

– Waste collection

– Electrical supply

– Computer

(optional)

– Safety and installation materials

• If possible, moves the instrument packages to the site before the installation date.

• Is available during installation to assist service representative and laboratory personnel.[1]

• Ensures that at least two people are available to help the service representative move and

position the instrument.[1]

Network or

IT specialist

(if the instrument

will be connected

to a network)

• Ensures that active, tested local area network (LAN) connections are in place.

• Ensures that network hardware is compatible with an RJ45-type connector.

• If necessary, supplies additional cables.

• Is available during installation to connect the instrument to the network.[1]

• If applicable, provides and installs a network or dedicated printer.

• Ensures that the

QuantStudio

™

3 and 5 Real-Time PCR Systems IT Checklist

(Pub. No. MAN0013738) has been completed and returned.

[1] Required for service representative installation of the instrument.

Customer responsibilities

QuantStudio

™

3 and 5 Real-Time PCR Systems Site Preparation Guide | MAN0010405 C.0

Site requirements

Note: All measurements are rounded to the nearest whole- or half-unit.

Components (packaged)

WARNING! PHYSICAL INJURY HAZARD. The packages are heavy. Do not

aempt to lift or move the packages without professional assistance. Any

incorrect lifting or moving of the packages can cause serious injury.

Ensure the building clearances allow for the passage of the instrument packaging.

Package Height Length (depth) Width Weight

Instrument (on pallet) 63 cm (25 in.) 69 cm (27 in.) 42 cm (16.5 in.) 35 kg (77 lb)

Computer (laptop)[1] 22 cm (9 in.) 42 cm (16.5 in.) 59 cm (23 in.) 7 kg (15 lbs)

Computer (minitower)[1] 36 cm (14 in.) 47 cm (18.5 in.) 54 cm (21 in.) 14.5 kg (32 lb)

Monitor (with stand)[1] 18 cm (7 in.) 46 cm (18 in.) 41 cm (16 in.) 7 kg (15 lbs)

[1] Acomputer (laptop or minitower), monitor, and keyboard are optional equipment.

Components (unpacked)

Ensure that the installation site bench space can accommodate the dimensions and

support the weights of the purchased conguration.

Component Height Length (depth) Width Weight

Instrument 40 cm (16 in.) 50 cm (20 in.) 27 cm (10.5 in.) 26 kg (57 lbs)

Computer (laptop)[1] 4 cm (1.5 in.) 25 cm (10 in.) 38 cm (15 in.) 2.5 kg (5 lbs)

Computer (minitower)[1] 36 cm (14 in.) 42 cm (16.5 in.) 17.5 cm (7 in.) 9.5 kg (21 lbs)

Monitor (with stand)[1] 37 cm (15 in.) 18 cm (7 in.) 41 cm (16 in.) 5 kg (11 lbs)

Keyboard[1] 3 cm (1.5 in.) 14 cm (5.5 in.) 45 cm (18 in.) 1 kg (2.5 lbs)

[1] Acomputer (laptop or minitower), monitor, and keyboard are optional equipment.

During instrument installation and maintenance, it is necessary to access the back of

the instrument. If the back of the instrument faces a wall, ensure that there is sucient

clearance on the bench to rotate the instrument for access.

IMPORTANT! For safety, the power outlet for the instrument must be accessible.

Component Top Front Sides Back

Instrument 30 cm (12 in.) 30 cm (12 in.) 15 cm (6 in.) 15 cm (6 in.)

Computer[1] — 15 cm (6 in.) — 15 cm (6 in.)

[1] Co-locating the computer with the instrument is optional.

Dimensions and

weights

Instrument

clearances

Site requirements

QuantStudio

™

3 and 5 Real-Time PCR Systems Site Preparation Guide | MAN0010405 C.0

Figure 1 Instrument back panel

1USB ports

2WiFi USB port—Connect USB wireless

adapter for wireless network access

(ordered separately)

3Ethernet Port—RJ45 port for

100/1,000 Mbps Ethernet communication

with the instrument

4RS232 Port—For service use only

5Fuse Cover

6Power Switch

7Power Port—100 to 240 VAC

1 1

Figure 2 Instrument‑to‑computer connections (minitower conﬁguration)

1Detachable power supply cord compatible with local power supply receptacle.

2Connection between the computer and the instrument.

3Connection between the computer and the monitor, keyboard, and mouse.

4Connection between the computer and the

(optional)

handheld barcode scanner.

Instrument and

computer

connections

Site requirements

QuantStudio

™

3 and 5 Real-Time PCR Systems Site Preparation Guide | MAN0010405 C.0

Table 1 Environmental requirements

Condition Acceptable range

Installation site Indoor use only

Electromagnetic

interference

Do not use this device in close proximity to sources of strong

electromagnetic radiation (for example, unshielded intentional

RF sources). Strong electromagnetic radiation may interfere

with the proper operation of the device.

Altitude Between sea level and 2000 m (6500 ft.) above sea level

Operating conditions • Humidity: 15–80% relative humidity (noncondensing)

• Temperature: 15 to 30°C (59 to 86°F)

Note: For optimal performance, avoid rapid or extreme

ﬂuctuations in room temperature.

Storage and transport

conditions

• Humidity: 20–80% relative humidity (noncondensing)

• Temperature: –30 to 60°C (–22 to 140°F)

Thermal output During operation, the net thermal output, based on the actual

current draw of the instrument, is expected to be

approximately 960 W (3275 Btu/h).

Vibration Ensure that the instrument is not adjacent to strong vibration

sources, such as a centrifuge, pump, or compressor. Excessive

vibration will affect instrument performance.

Pollution degree The instrument has a Pollution Degree rating of II. The

instrument may only be installed in an environment that has

nonconductive pollutants such as dust particles or wood chips.

Typical environments with a Pollution Degree II rating are

laboratories and sales and commercial areas.

The noise output of the instrument is £60 dB when running.

Other conditions Ensure the instrument is located away from any vents that

could expel particulate material onto the instrument

components.

Avoid placing the instrument and computer adjacent to

heaters, cooling ducts, or in direct sunlight.

Environmental

requirements

Site requirements

QuantStudio

™

3 and 5 Real-Time PCR Systems Site Preparation Guide | MAN0010405 C.0

WARNING! For safety, the power outlet used for powering the instrument

must be accessible at all times. See “Instrument clearances“ on page 5 for

information about the space needed between the wall and the instrument. In

case of emergency, you must be able to immediately disconnect the main power

supply to all the equipment. Allow adequate space between the wall and the

equipment so that the power cords can be disconnected in case of emergency.

• Electric receptacle with grounding capability

•Maximum power dissipation: ~960 W (not including computer and monitor)

• Mains AC line voltage tolerances must be up to ±10 percent of nominal voltage

Device Rated voltage Circuit

required

Rated

frequency

Rated

power

Instrument 100–240 ±10% VAC[1] 10 A 50/60 Hz 960 W

Computer (laptop) 100–240 ±10% VAC 10 A 50/60 Hz 90 VA

Computer (desktop)

100–240 ±10% VAC 10 A 50/60 Hz

125 VA

Monitor 65 VA

[1] If the supplied power fluctuates beyond the rated voltage, a power line regulator may be required. High or low

voltages can adversely affect the electronic components of the instrument.

Electrical

requirements

Site requirements

QuantStudio

™

3 and 5 Real-Time PCR Systems Site Preparation Guide | MAN0010405 C.0

We recommend the use of electrical protective devices to protect the system in

environments with large voltage and power uctuations.

Recommended devices

Power line regulator

• 1.5-kVA power line regulator

• Use in areas where the supplied power ﬂuctuates in excess of ±10% of the normal voltage.

• Power ﬂuctuations can adversely affect the function of the instrument and computer.

Note: A power line regulator monitors the input current and adjusts the power supplied to the instrument or

computer. It does not protect against a power surge or failure.

Surge protector

• 10-kVA surge protector (line conditioner)

• Use in areas with frequent electrical storms or near devices that are electrically noisy, such as refrigerators, air

conditioners, or centrifuges.

• Short-duration, high-voltage power ﬂuctuations can abruptly terminate the function of, and thereby damage the

components of, the computer and the instrument.

Note: A dedicated line and ground between the instrument, computer, and the building’s main electrical service can

also prevent problems caused by power ﬂuctuations.

Uninterruptible power supply (UPS)

• 1.5-kVA uninterruptible power supply (UPS)

• Use in areas prone to power failure.

• Power failures and other events that abruptly terminate the function of the instrument and computer can

corrupt data and possibly damage the system.

WARNING! PHYSICAL INJURY HAZARD. Do not attempt to lift the UPS unit without assistance of at least

two people. Improper lifting can cause painful and permanent back injury. Refer to the UPS manufacturer

user guide for more information.

IMPORTANT! A UPS provides power for a limited time. It is meant to delay the effects of a power outage, not to

serve as a replacement power source. In the event of a power loss, power off the instrument and computer unless

you expect to regain power within the battery life of the UPS.

Electrical

protective devices

Site requirements

QuantStudio

™

3 and 5 Real-Time PCR Systems Site Preparation Guide | MAN0010405 C.0

Instrument-to-computer connections

We support the following direct, networked (LAN– local area network), or Cloud

congurations. Congurations other than those listed are not recommended. Select a

conguration that meets the needs of your laboratory's instrument, software, and

workow requirements.

IMPORTANT! Do not connect both an Ethernet cable and the Wi-Fi module

(Cat. No. A26774) to the instrument. Conguring the instrument for both wired and

wireless connection can interfere with instrument operation.

Note: For detailed information about networking your instrument, see the

QuantStudio™ 3 and 5 Real-Time PCR Systems IT Checklist (Pub. No. MAN0013738).

Direct conﬁguration option

• A computer provided by Thermo Fisher Scientiﬁc with the QuantStudio™Design and Analysis desktop Software

• Computer‑to‑instrument connection:

– Direct, wired connection between the computer and the instrument

using an Ethernet cable

IMPORTANT! A direct instrument‑to‑computer connection

cannot

be combined

with the LAN or the Cloud conﬁgurations.

Networked conﬁguration option

• A computer provided by Thermo Fisher Scientiﬁc with the QuantStudio™Design and Analysis desktop Software

• The computer and instrument must be on the same subnet mask.

• Computer‑to‑LAN connection

(select an option)

– Wired connection to the network using an Ethernet cable –

–

– Wireless connection to the network

LAN

• Instrument‑to‑LAN connection

(select an option)

– Wired connection to the network using an Ethernet cable –

–

– Wireless connection to the network using the instrument Wi‑Fi module

Thermo Fisher Cloud conﬁguration option

• Internet access and a computer with the Chrome™web browser to access the Cloud

• Computer‑to‑Cloud connection

(select an option)

– Wired connection to the internet using an Ethernet cable –

–

– Wireless connection to the internet

LAN

• Instrument‑to‑Cloud connection

(select an option)

– Wired connection to the network using an Ethernet cable –

–

– Wireless connection to the network using the instrument Wi‑Fi module

Supported options

for instrument

and computer

connections

Instrument-to-computer connections

QuantStudio

™

3 and 5 Real-Time PCR Systems Site Preparation Guide | MAN0010405 C.0

Este manual sirve para los siguientes modelos

Tabla de contenidos

Otros manuales de Equipo de laboratorio de Applied Biosystems

Applied Biosystems

Applied Biosystems 7500 Manual de usuario

Applied Biosystems

Applied Biosystems QuantStudio 3D Manual de usuario

Applied Biosystems

Applied Biosystems GeneAmp PCR System 9600 Manual de usuario

Applied Biosystems

Applied Biosystems 7500 Manual de usuario

Applied Biosystems

Applied Biosystems 7300 Manual de usuario

Applied Biosystems

Applied Biosystems ViiA 7 Real Manual de usuario

Applied Biosystems

Applied Biosystems GeneAmp PCR System 9700 Manual de usuario

Applied Biosystems

Applied Biosystems QuantStudio 6 Flex Manual de usuario

Applied Biosystems

Applied Biosystems CytoFluor 4000 Series Manual de usuario

Applied Biosystems

Applied Biosystems 7900HT Manual de usuario

Applied Biosystems

Applied Biosystems 7300 Manual del producto

Applied Biosystems

Applied Biosystems GeneAmp PCR System 9700 Manual de usuario

Applied Biosystems

Applied Biosystems StepOne Instrucciones de uso

Applied Biosystems

Applied Biosystems 2720 Manual de usuario

Applied Biosystems

Applied Biosystems A30207 Manual de usuario

Applied Biosystems

Applied Biosystems 2720 Manual de usuario

Manuales populares de Equipo de laboratorio de otras marcas

Qiagen

Qiagen DML 3000 Manual de usuario

Queensgate

Queensgate NANOSCAN OP400 Manual de programación

Conviron

Conviron GEN1000-GE Manual de usuario

Parr Instrument

Parr Instrument 4560 Manual de instrucciones

IKA

IKA IC control Manual de usuario

Integra

Integra DOSE IT Manual de usuario

Agilent Technologies

Agilent Technologies 5800 ICP-OES Manual de usuario

Endress+Hauser

Endress+Hauser Cleanfit CPA875 Manual de usuario

NI PXI-5422 Manual

Collomix

Collomix Aqix Manual de usuario

SPEX SamplePrep

SPEX SamplePrep 6875 Freezer/Mill Series Manual de usuario

Ocean Insight

Ocean Insight FLAME-NIR+ Manual de usuario

Parker

Parker ALIGN-MG-NA Manual de usuario

BD 644787 Manual de usuario

DENTAURUM

DENTAURUM Compact Megaplus Manual de usuario

Biuged Laboratory Instruments

Biuged Laboratory Instruments BGD 626 Manual de usuario

VWR

VWR SAS Super IAQ Manual de usuario

illumina

illumina MiSeqDx Manual de usuario