Accu-Scope EXI-300 Series Manual de usuario

MANUAL

EXI-300

INVERTED

MICROSCOPE SERIES

EXI-300 INVERTED MICROSCOPE SERIES

ACCU-SCOPE®2

CONTENTS

SAFETY NOTES ..................................................................................................................3

CARE AND MAINTENANCE................................................................................................3

INTRODUCTION..................................................................................................................4

UNPACKING AND COMPONENTS.....................................................................................4

COMPONENT DIAGRAMS..................................................................................................5

ASSEMBLY DIAGRAM.........................................................................................................6

DETAILED ASSEMBLY.....................................................................................................7-8

OPERATION

ILLUMINATION.......................................................................................................................... 9

INTERPUPILLARY DISTANCE.................................................................................................. 9

FOCUSING................................................................................................................................ 9

ADJUSTABLE TENSION CONTROL........................................................................................10

STAGE PLATES (OPTIONAL) .................................................................................................10

SELECTING THE LIGHT PATH................................................................................................11

APERTURE DIAPHRAGM ADJUSTMENT ...............................................................................12

PHASE CONTRAST (OPTIONAL) ..................................................................................... 12-14

MOUNTING A MICROSCOPY CAMERA (OPTIONAL) ............................................................14

TROUBLESHOOTING...................................................................................................15-17

MAINTENANCE..................................................................................................................18

SERVICE............................................................................................................................18

WARRANTY.......................................................................................................................18

EXI-300 INVERTED MICROSCOPE SERIES

ACCU-SCOPE®3

SAFETY NOTES

1. Open the shipping carton carefully to prevent any accessory, i.e. objectives or eyepieces, from

dropping and being damaged.

2. Do not discard the molded shipping carton; the container should be retained should the microscope

ever require reshipment.

3. Keep the instrument out of direct sunlight, high temperature or humidity, and dusty environments.

Ensure the microscope is located on a smooth, level and firm surface.

4. If any specimen solutions or other liquids splash onto the stage, objective or any other component,

disconnect the power cord immediately and wipe up the spillage. Otherwise, the instrument may be

damaged.

5. All electrical connectors (power cord) should be inserted into an electrical surge suppressor to

prevent damage due to voltage fluctuations.

6. For safety when replacing the LED lamp or fuse, be sure the main switch is off (“O”), remove the

power cord, and replace the LED bulb after the bulb and the lamp house has completely cooled.

7. Confirm that the input voltage indicated on your microscope corresponds to your line voltage. The

use of a different input voltage other than indicated will cause severe damage to the microscope.

CARE AND MAINTENANCE

1. Do not attempt to disassemble any component including eyepieces, objectives or focusing assembly.

2. Keep the instrument clean; remove dirt and debris regularly. Accumulated dirt on metal surfaces

should be cleaned with a damp cloth. More persistent dirt should be removed using a mild soap

solution. Do not use organic solvents for cleansing.

3. The outer surface of the optics should be inspected and cleaned periodically using an air stream from

an air bulb. If dirt remains on the optical surface, use a soft cloth or cotton swab dampened with a

lens cleaning solution (available at camera stores). All optical lenses should be swabbed using a

circular motion. A small amount of absorbent cotton wound on the end of a tapered stick such as

cotton swabs or Q-tips, makes a useful tool for cleaning recessed optical surfaces. Avoid using an

excessive amount of solvents as this may cause problems with optical coatings or cemented optics

or the flowing solvent may pick up grease making cleaning more difficult. Oil immersion objectives

should be cleaned immediately after use by removing the oil with lens tissue or a clean, soft cloth.

4. Store the instrument in a cool, dry environment. Cover the microscope with the dust cover when not

in use.

5. ACCU-SCOPE®microscopes are precision instruments which require periodic preventative

maintenance to maintain proper performance and to compensate for normal wear. An annual

schedule of preventative maintenance by qualified personnel is highly recommended. Your

authorized ACCU-SCOPE® distributor can arrange for this service.

EXI-300 INVERTED MICROSCOPE SERIES

ACCU-SCOPE®4

INTRODUCTION

Congratulations on the purchase of your new ACCU-SCOPE® microscope. ACCU-SCOPE®

microscopes are engineered and manufactured to the highest quality standards. Your microscope will

last a lifetime if used and maintained properly. ACCU-SCOPE® microscopes are carefully assembled,

inspected and tested by our staff of trained technicians in our New York facility. Careful quality control

procedures ensure each microscope is of the highest quality prior to shipment.

UNPACKING AND COMPONENTS

Your microscope arrived packed in a molded shipping carton. Do not discard the carton: the carton

should be retained for reshipment of your microscope if needed. Avoid placing the microscope in dusty

surroundings or in high temperature or humid areas as mold and mildew will form. Carefully remove the

microscope from the EPE foam container by its arm and base and place the microscope on a flat,

vibration-free surface. Check the components against the following standard configuration list:

1. Stand, which includes the supporting arm, focusing mechanism, nosepiece, mechanical stage

(optional), condenser with iris diaphragm, illumination system, and phase contrast accessories

(optional).

2. Binocular viewing head

3. Eyepieces as ordered

4. Objectives as ordered

5. Stage plate inserts, green and yellow filters (optional)

6. Dust cover

7. 3-prong electric power cord

8. Camera adapters (optional)

Optional accessories such as optional objectives and/or eyepieces, slides sets, etc., are not shipped as

part of the standard equipment. These items, if ordered, are shipped separately.

EXI-300 INVERTED MICROSCOPE SERIES

ACCU-SCOPE®5

COMPONENTS DIAGRAM

5w LED

Lamp House

Stand

Binocular

Viewing Head

Light split control

knobs

Camera/Photo

Port

Tension Adjustment Collar

Frame/Stand

Objective

Nosepiece

Coarse/Fine Focus

Adjustment Knob

Base

Eyepieces

Filter Holder

Phase Contrast Slider

(Optional)

Condenser

Stage

Camera/Photo

Port

On/Off Switch

Light Intensity Control Knob

X-Y Attachable

Mechanical Stage

(Optional)

Auxiliary Stage

(Optional)

EXI-300 INVERTED MICROSCOPE SERIES

ACCU-SCOPE®6

ASSEMBLY DIAGRAM

The diagram below shows how to assemble the various components. The numbers indicate the order of

assembly. Use the 1.5mm and 2mm hex wrenches that are supplied with your microscope when

required. Be sure to keep these wrenches for changing out components or making adjustments.

When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid scratching

any parts or touching glass surfaces.

①

② & ③

⑤

④

⑥

EXI-300 INVERTED MICROSCOPE SERIES

ACCU-SCOPE®7

ASSEMBLY

Objectives (Fig. 1 & 2)

To install the objectives:

1. Turn the coarse adjustment knob ①(Fig. 1) until the revolving nosepiece is at its lowest position.

2. Remove the nosepiece cap ② closest to you and thread the lowest magnification objective onto the

nosepiece opening, then rotate the nosepiece clockwise and thread the other objectives from low to

high magnification. (Fig. 2)

NOTE: ●Always rotate the nosepiece by using the knurled nosepiece ring.

●Keep the covers on any unused nosepiece openings to prevent dust and dirt from

getting inside.

Filters (Optional) (Fig. 3)

Take out the filter holder ①and insert a

filter ②. Be sure to push the filter down

to the bottom as shown ③. If it’s not

installed properly (i.e., it’s tilted in the

holder ④), it may fall through the filter

holder.

More than one filter can be stacked in the

filter holder as long as the total thickness

of all filters does not exceed 11mm.

Fig. 1

Fig. 2

Fig. 3

EXI-300 INVERTED MICROSCOPE SERIES

ACCU-SCOPE®8

Stage Plate (Fig. 4)

Insert the clear glass stage plate ①into the opening on the

stage. The clear glass allows you to view the objective in

position.

Eyepieces (Fig. 5)

Remove the eyetube plugs ①and insert the eyepieces②into

the eyepiece tubes ③. Lock in the eyepieces using the supplied

1.5mm hex wrench to tighten the screw on each eyetube.

Power Cord

VOLTAGE CHECK

Confirm that the input voltage indicated on the rear label of the

microscope corresponds to your line voltage. The use of a

different input voltage than indicated will cause severe

damage to your microscope.

Connecting the Power Cord

Make sure the On/Off Switch is “O” (the off position) before

connecting the power cord.

Insert the power plug into the power jack of the microscope;

make sure the connection is snug.

Plug the power cord into a power supply receptacle.

NOTE: Always use the power cord that came with your microscope. If your power cord becomes

damaged or lost, please call your authorized ACCU-SCOPE dealer for a replacement.

Fig. 4

Fig. 5

EXI-300 INVERTED MICROSCOPE SERIES

ACCU-SCOPE®9

OPERATION

Plug the 3-prong line cord into the microscope and then into a grounded 120V or 220V A.C. electrical outlet.

Usage of a surge suppressor outlet is highly recommended. Turn the illuminator switch ①to “―” (Fig. 6) For

longer bulb life always turn the illuminator variable intensity knob to the lowest illumination intensity setting

possible before turning the power on or off.

Adjusting the Illumination (Fig. 6)

The light level may need adjustment depending upon the

specimen density and objective magnification. Adjust the

light intensity for comfortable viewing by turning the light

intensity control knob clockwise to increase brightness.

Turn counterclockwise to decrease brightness.

Adjusting Interpupillary Distance (Fig. 7 & 8)

To adjust the interpupillary distance, hold the left and right

eyetubes while observing a specimen. Rotate the eyetubes

around the central axis until the fields of view of both eyetubes

coincide completely. A complete circle should be seen in the

viewing field when viewing the specimen slide. An improper

adjustment will cause operator fatigue and will disrupt the

objective parfocality.

Where the “●” ①on the eyepiece tube lines up, that is the

number for your interpupillary distance. The range is 48-75mm.

Be sure to write down you interpupillary number for future

operation.

Adjusting the Focus (Fig. 8)

To ensure that you obtain sharp images with both eyes, (since

eyes vary, especially for those wearing glasses) any eyesight

variation can be corrected in the following manner. Set both

diopter collars ②to “0”. Using your left eye only and the 10X

objective, focus your specimen by adjusting the coarse

adjustment knob. When the image is in view, refine the image

to its sharpest focus by turning the fine adjustment knob.

Rotate the diopter collar to obtain the sharpest focus. To obtain

the same sharp image using your right eye, do not touch the

coarse or fine adjustments. Instead, rotate the right diopter

collar until the sharpest image appears. Repeat several times

to check.

IMPORTANT: do not counter rotate the focusing knobs as this will

cause severe problems and damage to the focusing system.

Fig. 7

②

①

Fig. 8

②

①

Fig. 6

EXI-300 INVERTED MICROSCOPE SERIES

ACCU-SCOPE®10

Operation (continued)

Adjusting the Focusing Tension (Fig. 9)

If the feel is very heavy when focusing with the focusing knobs

②③, or the specimen leaves the focus plane after focusing,

or the stage lowers by itself, adjust the tension with the tension

adjustment ring ①.

Turn the tension adjustment ring clockwise to loosen or

counterclockwise to tighten according to user preference. (Fig.

9).

Using the Stage Plates (Optional) (Fig. 10 & 11)

NOTE: for optimal viewing, ensure the thickness marked on

each objective (0.17mm or 1.2mm) is the same as the

container, dish or slide.

With the mechanical stage ②, a user can use any of the

optional stage plates for flasks, well plates, culture dishes or

slides. The appropriate stage is inserted into the slide holder

and can be moved by turning the X③and Y ④stage

movement controls.

Fig. 10

Fig. 9

②

①

④

③

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