10x Genomics Chromium Single Cell Controller Readiness... Manual de usuario

Library Construction

Chromium™

Single Cell Controller

Readiness Test

FOR USE WITH

Chromium™ Single Cell Controller & Accessory Kit

PN-120263

NOTICES

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Chromium™ Single Cell Controller Readiness Test 1

Notices

Manual Part Number

CG00051 Rev A

Legal Notices

document without the express written consent of 10x Genomics, Inc., is strictly forbidden. Nothing contained

herein shall constitute any warranty, express or implied, as to the performance of any products described

herein. Any and all warranties applicable to any products are set forth in the applicable terms and

conditions of sale accompanying the purchase of such product. 10x Genomics provides no warranty and

hereby disclaims any and all warranties as to the use of any third party products or protocols described

herein. The use of products described herein is subject to certain restrictions as set forth in the applicable

terms and conditions of sale accompanying the purchase of such product. “10x”, “10x Genomics”, “Changing

the Definition of Sequencing”, “Chromium”, “GemCode”, “Loupe”, “Long Ranger”, “Cell Ranger” and

“Supernova” are trademarks of 10x Genomics, Inc. All other trademarks are the property of their respective

owners. All products and services described herein are intended FOR RESEARCH USE ONLY and NOT FOR

USE IN DIAGNOSTIC PROCEDURES.

Customer Information and Feedback

For technical information or advice, please contact our Customer Technical Support Division online at any

time.

Email: support@10xgenomics.com

10x Genomics

7068 Koll Center Parkway

Suite 401

Pleasanton, CA 94566 USA

READINESS TEST

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Chromium™ Single Cell Controller Readiness Test 2

Chromium™ Controller Accessory Kit

Product Description # Part Number

Chromium™ Controller Accessory Kit 110204

Power Cord 1 34000X

10x™ Vortex Adapter 3 330002

10x™ Chip Holder 2 330019

10x™ Gasket (3 pack) 1 -

10x™ Vortex Clip 2 230002

10x™ Magnetic Separator 2 230003

Chromium Test Chip V1 1 230024

READINESS TEST

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Chromium™ Single Cell Controller Readiness Test 3

1. Turn on the Chromium™ Single Cell Controller

a) Ensure the Chromium Single Cell Controller is positioned in accordance with

recommendations in the Chromium Single Cell Controller Specifications.

b) Turn on the Chromium Single Cell Controller using the power switch on the back of the

unit.

c) The Chromium Single Cell Controller touchscreen will indicate initialization is in

progress.

d) When initialization is complete, press the button on the touchscreen of the Chromium

Single Cell Controller to eject the tray and remove the foam strip used for protection

during shipping. Press the button again to retract the tray (or the tray will automatically

close after 40 sec).

READINESS TEST

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Chromium™ Single Cell Controller Readiness Test 4

2. Assemble a Test Chip and a 10x™ Chip Holder

a) Align the notch on the upper left corner of the Test Chip with the notch on the 10x Chip

Holder and insert the left-hand side of the Test Chip under the guide.

b) Depress the right-hand side of the Test Chip until the spring-loaded clip engages the

Test Chip.

c) Close the hinged lid of the 10x Chip Holder. After loading the Test Chip, the 10x Chip

Holder should lay flat on the bench top with the lid closed.

d) Orient the assembly so that the Partitioning Oil wells (row labeled 3) are toward you.

READINESS TEST

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Chromium™ Single Cell Controller Readiness Test 5

3. Attach a Gasket & Run the Chromium™ Single Cell

Controller

3.1. Attach a 10x™ Gasket

a) The notched cut of the 10x Gasket should be at the top left corner. Hold the 10x Gasket

by the tongue and hook it on the left-hand tabs of the 10x Chip Holder. Gently pull the

10x Gasket toward the right and hook it on the two right-hand tabs. Ensure the 10x

Gasket holes are aligned with the wells. Avoid touching the smooth side of the 10x

Gasket and do not press down on the top of the 10x Gasket after attachment.

3.2. Run the Chromium™ Single Cell Controller

a) Press the button on the touchscreen of the Chromium Single Cell Controller to eject the

tray.

Touch the touchscreen to wake up the

Chromium Single Cell Controller

Press the eject button to eject the tray

READINESS TEST

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Chromium™ Single Cell Controller Readiness Test 6

b) Place the Test Chip assembled in 10x™ Chip Holder with a 10x™ Gasket installed on the

tray (align the diagonal etchings on the top left corner of the 10x Chip Holder and the

back left corner of the tray).

c) Press the button to retract the tray. Confirm the Chromium™ Test program shows on

screen and press the play button to begin the run.

Place the assembled Chip, 10x Chip

Holder and 10x Gasket in the tray and

press the button to retract the tray

Confirm the Chromium Test program

shows on the screen and press the

play button to start the run

d) At the completion of the run (3 min), the Chromium Single Cell Controller will chime.

Press the button to eject the tray. Remove and disassemble the Chip Holder assembly.

Press the button again to retract the empty tray (or the tray will automatically close

after 40 sec).

After the run is completed, press the

eject button to eject the tray

Remove the Chip Holder assembly and

press the button again to retract the

tray (or the tray will automatically

close after 40 sec)

e) If there are any problems during this process, contact support@10xgenomics.com for

further assistance.

f) Save the 10x Chip Holder for use with your Chromium Single Cell Controller. The 10x

Gasket and Test Chip should also be stored.

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